Characterization of Glutamate Decarboxylase from a High γ-Aminobutyric Acid (GABA)-Producer,<i>Lactobacillus paracasei</i>

Komatsuzaki, Noriko; Nakamura, Toshihide; Kojima, Toshinori; Shima, Jun

doi:10.1271/bbb.70163

Cited by 145 publications

(101 citation statements)

References 31 publications

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“…Further increase in the pH led to a decrease of GABA production. These results accorded with the previous reports about the optimal pH values for maintaining the activity of LAB GAD were in the range of 4.0-5.0 (Huang et al, 2007;Komatsuzaki et al, 2008). The higher or lower pH may lead to the partial loss of the GAD activity.…”

Section: Effect Of Initial Phsupporting

confidence: 92%

Enhancement of γ-Amminobutyric Acid Production by Co-Culturing of Two Lactobacilli Strains

Gomaa¹

2015

Asian J. of Biotechnology

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The γ-Amino Butyric Acid (GABA) is a major inhibitory neurotransmitter in central nervous system and its application in drugs and functional foods has attracted great attention. Most of the fermented food products are known and proven for its high content GABA producer, which makes the food product as potential functional foods. Lactic acid bacteria possess special physiological activities and are generally regarded as safe. This study was aimed at evaluating GABA production ability of some lactic acid bacteria strains isolated from Egyptian dairy products and further to increase the production by combining the best GABA producers. The impacts of pH, temperature, incubation time, initial monosodium glutamate (MSG) concentration and pyridoxal-5'-phosphate (PLP) addition on growth and γ-aminobutyric acid production were investigated. By optimizing these factors at pH5, cultivation temperature of 35°C, cultivation time of 72 h in medium supplemented with 750 M of MSG and 200 μM of PLP, Lactobacillus brevis NM101-1 and Lactobacillus plantarum DSM749 accumulated 168.58 and 140.69 mM of GABA, respectively. Co-culturing of both strains was not only able to enhance GABA production but also producing it in a short time (48 h), reached 224.69 mM. It could be concluded that the use of LAB strains in combination with a high GABA producer strain is a successful approach for reaching a physiologically active concentration of GABA.

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Section: Effect Of Initial Phsupporting

confidence: 92%

Enhancement of γ-Amminobutyric Acid Production by Co-Culturing of Two Lactobacilli Strains

Gomaa¹

2015

Asian J. of Biotechnology

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“…pH (6-8) and temperature (30-40°C ) were also selected as independent variables based on the previous literature (Katikala, Bobbarala, Tadimalla, & Guntuku, 2009;Vermeulen, Gänzle, & Vogel, 2007;Weingand-Ziadé, Gerber-Décombaz, & Affolter, 2003). Second-stage fermentation for GABA production also involved the selection of three factors including pylidoxal-5-phosphate (PLP), cofactor, concentration (0-0.05 mM) (Bai et al, 2009;Komatsuzaki, Nakamura, Kimura, & Shima, 2008), pH (4.5-6.5) (Cho, Chang, & Chang, 2007;Komatsuzaki et al, 2008) and temperature (30-40°C) (Cho et al, 2007;Lu et al, 2009;Ratanaburee et al, 2011).…”

Section: Description Of the Experimental Designmentioning

confidence: 99%

Evaluation of factors that influence the L-glutamic and γ-aminobutyric acid production duringHericium erinaceusfermentation by lactic acid bacteria

Woraharn

Lailerd

Sivamaruthi

et al. 2015

CyTA - Journal of Food

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“…Additionally, in rats subjected chronically to the forced swim test, B. infantis administration triggered a significant increase in plasma tryptophan concentration, known as a serotonergic precursor [108]. Another probiotic, L. paracasei, has been shown to produce neurotransmitter GABA [159]. In addition, probiotics can also exert anti-stress effects by decreasing the level of cortisol, a well-characterized stress hormone [160].…”

Section: Probioticsmentioning

confidence: 99%

Gut Microbiome-Brain Communications Regulate Host Physiology and Behavior

Lee¹,

Serre²

2015

JNHFS

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cell injury [6], metabolic regulation [7], GI tract development [8], innate and adaptive immune responses, and absorption of nutrients [9,10]. Alterations in microbiota composition and dysregulation of the intestinal mucosa homeostasis have been implicated in the development and progression of pathologies. This compositional change in the microbiota and/ or an abnormality in the interactions between the host and the commensal microbiota is referred to as dysbiosis. Gut microbiota dysbiosis has been linked to chronic low-grade intestinal inflammation and acute intestinal autoimmunity diseases such as Irritable Bowel Syndrome (IBS) and Inflammatory Bowel Disease (IBD) [11,12]. Abnormal microbiota composition is associated with a wide range of metabolic and behavioral disorders, such as anxiety/depression [13][14][15] [26]. GI microbiota dysbiosis might also be involved in the development and persistence of systemic disorders [27]. For example, obesity has been characterized by a decrease in overall diversity [28] and an increase in Proteobacteria abundance and in the Firmicutes to Bacteroidetes ratio [28,29] and microbiota composition is believed to influence energy balance and glucose homeostasis [30][31][32].Evidence that changes in microbiota composition are correlated with metabolic and behavioral disorders has drawn attention to a potential causal role for the microbiota in pathologies and has led to the emergence of the 'microbiota-gutbrain axis' concept [33][34][35]. The gut-brain axis is a bidirectional communication system between the GI tract and the brain [36] via hormonal, immunological, and neural signaling. Information from the GI tract and the intestinal microbiota can reach the peripheral and Central Nervous System (CNS), concurrently the brain is able to influence GI functions such as motility and secretion but also immune responses and cytokine production [36,37]. The Microbiota-Gut-Brain AxisThe gut microbiota can modulate gut-brain axis signaling via direct and indirect mechanisms. The microbiota acts via endocrine, metabolic (bacterial components and metabolites), AbstractThe human gut microbiota contains more than 100 trillion bacteria that, under normal physiological conditions, have beneficial symbiotic interactions with the host. However, a growing body of evidence has shown that alternations in the composition and diversity of the gut microbiota, or dysbiosis, can influence the development and progress of metabolic and neurological disorders. Communication between the microbiota and the brain is a bidirectional system involving endocrine, metabolic (bacterial components and metabolites), immune, and neural pathways. Gut microbiota composition influences the signals transmitted from the gut to the brain. Alternatively, the brain utilizes similar mechanisms, in particular endocrine and neural signaling, to modulate the composition of the gut bacteria. In this review, we describe the recent evidence of gut microbiota interaction with the central nervous system to influence physiological a...

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Characterization of Glutamate Decarboxylase from a High γ-Aminobutyric Acid (GABA)-Producer,Lactobacillus paracasei

Cited by 145 publications

References 31 publications

Enhancement of γ-Amminobutyric Acid Production by Co-Culturing of Two Lactobacilli Strains

Enhancement of γ-Amminobutyric Acid Production by Co-Culturing of Two Lactobacilli Strains

Evaluation of factors that influence the L-glutamic and γ-aminobutyric acid production duringHericium erinaceusfermentation by lactic acid bacteria

Gut Microbiome-Brain Communications Regulate Host Physiology and Behavior

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