Characterization of Gold Nanoparticles Modified with Single-Stranded DNA Using Analytical Ultracentrifugation and Dynamic Light Scattering

Falabella, James B.; Cho, Tae Joon; Ripple, Dean C.; Hackley, Vincent A.; Tarlov, Michael J.

doi:10.1021/la100761f

Cited by 50 publications

(60 citation statements)

References 31 publications

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“…To use this approach, the density is usually assumed to be equal to the materials bulk density. There are reports that this is not always true, with measured values being less than the bulk value [45]. A 2-fold error in density, perhaps larger than expected for bulk versus nanoparticle, has the same effect on diameter calculation as a 50 % loss of NPs in the standard solution when using method 2 (i.e., a 25 % overestimate of diameter).…”

Section: Size Calibration By Nanoparticle Standards and Microdropletsmentioning

confidence: 95%

“…Recent studies have shown that the density of some particle compositions may be size-dependent [59,60]. Falabella et al demonstrate through analytical ultracentrifugation that as the size of gold NPs decrease, the density also decreases [45]. The potential for size-dependent particle density is an emerging aspect of spICP-MS that needs further consideration as it continues to develop into a routine analysis technique.…”

Section: Conversion Of Spicp-ms Signal To Np Sizementioning

confidence: 99%

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Single Particle ICP-MS: Advances toward routine analysis of nanomaterials

et al. 2016

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From its early beginnings in characterizing aerosol particles to its recent applications for investigating natural waters and waste streams, single particle inductively coupled plasma-mass spectrometry (spICP-MS) has proven to be a powerful technique for the detection and characterization of aqueous dispersions of metal-containing nanomaterials. Combining the high-throughput of an ensemble technique with the specificity of a single particle counting technique and the elemental specificity of ICP-MS, spICP-MS is capable of rapidly providing researchers with information pertaining to size, size distribution, particle number concentration, and major elemental composition with minimal sample perturbation. Recently, advances in data acquisition, signal processing, and the implementation of alternative mass analyzers (e.g., time-of-flight) has resulted in a wider breadth of particle analyses and made significant progress toward overcoming many of the challenges in the quantitative analysis of nanoparticles. This review provides an overview of spICP-MS development from a niche technique to application for routine analysis, a discussion of the key issues for quantitative analysis, and examples of its further advancement for analysis of increasingly complex environmental and biological samples. Graphical Abstract Single particle ICP-MS workflow for the analysis of suspended nanoparticles.

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Section: Size Calibration By Nanoparticle Standards and Microdropletsmentioning

confidence: 95%

Section: Conversion Of Spicp-ms Signal To Np Sizementioning

confidence: 99%

Single Particle ICP-MS: Advances toward routine analysis of nanomaterials

et al. 2016

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“…Using this estimate in conjunction with the length of dsDNA as 3.4 Å/bp, we can also estimate an increase in radius following hybridization with complements A, B, or C to be in the 23.5À31.0 nm range, which is consistent with the measured range of 23.6À26.5 nm. If the ssDNA is assumed to be in a random coil configuration on the NP, we can apply an alternate treatment to estimate the size of the ssDNA by solving the mean square endto-end distance of a polymer AER 2 ae using a worm-like chain (WLC) model: 35,36 …”

Section: Articlementioning

confidence: 99%

Multimodal Characterization of a Linear DNA-Based Nanostructure

Buckhout‐White

Ancona²,

et al. 2012

ACS Nano

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Designer DNA structures have garnered much interest as a way of assembling novel nanoscale architectures with exquisite control over the positioning of discrete molecules or nanoparticles. Exploiting this potential for a variety of applications such as light-harvesting, molecular electronics, or biosensing is contingent on the degree to which various nanoarchitectures with desired molecular functionalizations can be realized, and this depends critically on characterization. Many techniques exist for analyzing DNA-organized nanostructures; however, these are almost never used in concert because of overlapping concerns about their differing character, measurement environments, and the disparity in DNA modification chemistries and probe structure or size. To assess these concerns and to see what might be gleaned from a multimodal characterization, we intensively study a single DNA nanostructure using a multiplicity of methods. Our test bed is a linear 100 base-pair double-stranded DNA that has been modified by a variety of chemical handles, dyes, semiconductor quantum dots, gold nanoparticles, and electroactive labels. To this we apply a combination of physical/optical characterization methods including electrophoresis, atomic force microscopy, transmission electron microscopy, dynamic light scattering, Förster resonance energy transfer, voltammetry, and structural modeling. In general, the results indicate that the differences among the techniques are not so large as to prevent their effective use in combination, that the data tend to be corroborative, and that differences observed among them can actually be quite informative.

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“…DNA nanostructures, combined with nanoparticles, have a potential to become a key technology in the production of nanoelectronics in the future. Conductive nanoparticles can also be functionalized with DNA structures [20] [21]. Application of such nanostructures are in the field of medicine [12], power harvesting [15] [22]and nanoelectronics [20].…”

Section: Dna Origamimentioning

confidence: 99%

Engineered nanoparticles for targeted drug delivery

Breland

Patel

Bajwa

2012

2012 IEEE Long Island Systems, Applications and Technology Conference (LISAT)

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DNA based nanostructures and advances in DNA origami techniques have shown great potential in fabrication and of nanostructures and devices. Though a large number of DNA origami structures have been reported [1-3], very few applications of DNA structures have been presented. Custom shapes formed by programmable DNA self assembly can be used to engineer nanoscale devices such as a biological antenna. The focus of our research is to design a nanoscale antenna using DNA biostructure as a scaffold. Conductivity of such a biological antenna can be achieved by using conductive nano-particles coating on DNA [4] or by attaching conducting polymers to DNA structures[5].In this paper, we will present design of engineered nano-antennas with well defined engineering characteristics. Engineered nanoantennas have a resonant frequency, that can be used for diagnostic and drug delivery. The ability of such antennas to resonate at a particular frequency will give researchers the ability to communicate and control the nanoparticles. Such nanoparticles are carriers that can be used as advanced detection systems to help identifying toxicity of nanoparticles in the body. Organic molecules, such as folic acid, can be easily bonded to the surface of antenna to detect cancerous cells. We will further discuss detailed DNA origami techniques that can be used to realize such nanostructures.

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Characterization of Gold Nanoparticles Modified with Single-Stranded DNA Using Analytical Ultracentrifugation and Dynamic Light Scattering

Cited by 50 publications

References 31 publications

Single Particle ICP-MS: Advances toward routine analysis of nanomaterials

Single Particle ICP-MS: Advances toward routine analysis of nanomaterials

Multimodal Characterization of a Linear DNA-Based Nanostructure

Engineered nanoparticles for targeted drug delivery

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