Characterization of granulocytes and mast cells in cultures of mouse bone marrow stimulated with interleukin‐3

Bender, James; Epps, Dennis E. Van; Stewart, C. C.

doi:10.1002/jcp.1041350110

Cited by 7 publications

(3 citation statements)

References 19 publications

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“…BMMC were generated essentially as described by Bender et al [37]. In short, unfractionated bone marrow cells from B6 or BALB.B mice were cultured at 2.5 × 10 5 cells/ml in supplemented RPMI 1640 medium containing 25 U/ml IL-3 (Pharmingen).…”

Section: Isolation Of Bone Marrow-derived and Peritoneal Mast Cellsmentioning

confidence: 99%

2F1 antigen, the mouse homolog of the rat “mast cell function-associated antigen”, is a lectin-like type II transmembrane receptor expressed by natural killer cells

Hanke¹,

Corral²,

Vance³

et al. 1998

Eur. J. Immunol.

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Inhibitory lectin-like receptors expressed on the surface of hematopoietic cells are critically involved in regulation of their effector functions. Here we report that a novel mAb specific for mouse NK cells, 2F1, recognizes the mouse homolog of the mast cell function-associated antigen (MAFA), an inhibitory lectin-like transmembrane receptor expressed on rat mast cells. The 2F1 antigen (2F1-Ag) and rat MAFA are structurally highly conserved and contain a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif that is presumably utilized for inhibitory signaling. We also identified a human homolog that is closely related to the rodent MAFA/2F1-Ag proteins. Like rat MAFA, 2F1-Ag is probably encoded by a single gene, which exhibits relatively little polymorphism. Strikingly, while rat MAFA is considered a mast cell antigen, we have been unable to detect cell surface expression of 2F1-Ag by mouse mast cell lines, bone marrow-derived mast cells, or peritoneal mast cells. Furthermore, mouse bone marrow-derived mast cells were devoid of 2F1-Ag mRNA. Instead, we find that approximately 40 % of mouse NK cells express 2F1-Ag. Thus, MAFA/ 2F1-Ag may modulate immunological responses on at least two different cell types bridging the specific and innate immune system. Abbreviations: BMMC: Bone marrow-derived mast cells ITIM: Immunoreceptor tyrosine-based inhibitory motif MAFA: Mast cell function-associated antigen Eur. J. Immunol. 1998. 28: 4409-4417 2F1-Ag expression by mouse NK cells

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Section: Isolation Of Bone Marrow-derived and Peritoneal Mast Cellsmentioning

confidence: 99%

2F1 antigen, the mouse homolog of the rat “mast cell function-associated antigen”, is a lectin-like type II transmembrane receptor expressed by natural killer cells

Hanke¹,

Corral²,

Vance³

et al. 1998

Eur. J. Immunol.

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“…In part, our results suggest a close association between rat marrow cells resembling members of the mast cell lineage and early hemopoietic stemiprogenitor cells by expressing functional c-kit receptors and having high levels of Thy 1.1 on their surface. Other studies have shown that cells capable of developing into marrow-derived mast cells by IL-3 stimulation have been isolated from mouse marrow in a Thy 1.2' subset (Bender et al, 1988) and in a subset of CD-34+ human marrow cell progenitors (Kirshenbaum et al, 1991). Moreover, others show that human stem cell factor synergizes with IL-3 to stimulate mast lineage cells from this CD34 ' marrow subset (Kirshenbaum et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

Isolation of rat bone marrow mast lineage cells using Thy 1.1 and rat stem cell factor

Medlock

Mineo

Housman

et al. 1992

Journal Cellular Physiology

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Recent reports have shown that various marrow-derived cell populations respond vigorously to recombinant rat stem cell factor (rrSCF164), one form of the kit-ligand. In the present study, we isolated cell populations from rat bone marrow using the Thy 1.1 antigen (an antigen that in the rat is differentially expressed on primitive hemopoietic progenitor cells) and fluorescently conjugated rrSCF164 (rrSCF164-PE). We show that rrSCF164 only stimulates cells that are enriched in the brightest Thy 1.1 populations (Thy 1.1bright). Numerous cell lines were generated by serial passage in rrSCF164 containing medium, and the prototypic cell lines have been designated SRT002 and SRT003. Each cell line retains the Thy 1.1bright phenotype and does not respond to interleukins (IL) 1-8, IL-10, granulocyte (G) colony-stimulating factor (CSF), granulocyte macrophage (GM) CSF, M-CSF, or crude preparations of mitogen-stimulated T-cell supernatants. The Thy 1.1bright population of rat marrow was subdivided into a subset that binds rrSCF164-PE (Thy 1.1bright, rrSCF164+). The majority of these cells possess certain characteristics in common with marrow-derived mast cells and the Thy 1.1bright, rrSCF164 responsive cell lines, having similar granule morphology, being metachromatic, and reacting positively with alcian blue. Moreover, rats treated with rrSCF164 displayed significant increases in Thy 1.1bright, rrSCF164+ cells in the bone marrow. These studies show that the combination of Thy 1.1 and rrSCF164 makes possible the isolation of a unique subset of rat bone marrow cells that differentially express the Thy 1.1 antigen and the cell surface receptor c-kit, the majority of which are morphologically similar to marrow-derived mast cells.

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“…It is capable of producing nearly pure colonies of immature Ma cells similar to MMC after 2-4 weeks in culture with IL-3 as the principal stimulant. The population of Ma-CFC appears to be Thyl-positive and to express a high-affinity IgE receptor (Bender, van Epps & Stewart 1988, Rottem et al 1992. Ma-CFC is producing moremature cells of higher density (over 1.12 g/cm3), which are IL-3-independent, morphologically similar to differentiated Ma, and form small and medium colonies in vitro (Kanaruka et al 1988).…”

Section: Kinetics O F Eosinophils Basophils a N D Mast Cellsmentioning

confidence: 99%

Proliferation‐differentiation pathways of murine haemopoiesis: correlation of lineage fluxes

Novák

Nečas

1994

Cell Proliferation

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A quantitative analysis of literature data on muiine bone marrow haemopoiesis was performed in order to determine how the haemolpoietic system fulfils requirements of cell turnover in the normal steady state. In particular, the production rates (fluxes) of erythrocytes, megakaryocytes, monocytes, neutrophils, eosinophils, basophils and mast cells have been calculated and normalized. Subsequently, based on available data on relationships of various lineages, a working model of the developmental schema of haemopoiesis was derived. Since, according to the principle of conservation, the effluxes of all lineages from bone marrow in the steady state must be equal to effluxes from the compartments of respective precursors divided by the coefficient of multiplication, the correlation of lineage fluxes was used to determine the position of branching points. It was concluded that all the quantita.tive data on lineage proliferation in the normal steady state in vivo, available for analysis,, are fully consistent with a simple binary model of the sequential type, similar to that suggested by Brown and coworkers (Brown G.

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Characterization of granulocytes and mast cells in cultures of mouse bone marrow stimulated with interleukin‐3

Cited by 7 publications

References 19 publications

2F1 antigen, the mouse homolog of the rat “mast cell function-associated antigen”, is a lectin-like type II transmembrane receptor expressed by natural killer cells

2F1 antigen, the mouse homolog of the rat “mast cell function-associated antigen”, is a lectin-like type II transmembrane receptor expressed by natural killer cells

Isolation of rat bone marrow mast lineage cells using Thy 1.1 and rat stem cell factor

Proliferation‐differentiation pathways of murine haemopoiesis: correlation of lineage fluxes

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