Characterization of growth and sporulation of Metarhizium anisopliae in solid-substrate fermentation

Dorta, Blas; Ertola, R. J.; Arcas, Jorge Alfredo

doi:10.1016/s0141-0229(96)00017-8

Cited by 42 publications

(25 citation statements)

References 25 publications

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“…D3 and D1 were equally efficient media for producing spores of C. minitans based on y sp . These values for D1 and D3 given in Table III were comparable with coefficients of 0.035 and 0.317 for y m and y sp , respectively, found for M. anisopliae grown on rice husk (Dorta et al, 1996).…”

Section: Stoichiometrysupporting

confidence: 86%

“…The ash content was assumed to be 5% for mycelium and 3% for spores based on results with P. roqueforti and M. anisopliae (Desfarges et al, 1987;Dorta et al, 1996).…”

Section: Elemental Compositionmentioning

confidence: 99%

“…This approach has been used successfully to identify nutrient limitations of Penicillium roqueforti growing on buckwheat (Larroche and Gros, 1992). A similar approach was found for the characterization of growth and sporulation of Metarhizium anisopliae (Dorta et al, 1996). In addition, elemental balances can be used to determine whether other byproducts besides mycelium and spores are formed.…”

Section: Introductionmentioning

confidence: 97%

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Growth and sporulation stoichiometry and kinetics ofConiothyrium minitans on agar media

Ooijkaas¹,

Buitelaar²,

Tramper³

et al. 2000

Biotechnol. Bioeng.

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Section: Stoichiometrysupporting

confidence: 86%

“…The ash content was assumed to be 5% for mycelium and 3% for spores based on results with P. roqueforti and M. anisopliae (Desfarges et al, 1987;Dorta et al, 1996).…”

Section: Elemental Compositionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

Growth and sporulation stoichiometry and kinetics ofConiothyrium minitans on agar media

Ooijkaas¹,

Buitelaar²,

Tramper³

et al. 2000

Biotechnol. Bioeng.

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“…[11][12][13] By contrast, solid-state fermentation is a promising alternative since conidia and metabolites are generally produced in higher quantities when these microorganisms are produced on a solid substrate. [11][12][13][14][15][16] For example, Aspergillus oryzae has been reported to produce a 500-fold higher yield of recombinant chymosin in solid-state fermentation than in submerged fermentation. 17) In this context, efficient utilization of agro-industrial residues as carbon sources has been shown for the mass production of entomopathogenic fungi.…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity of <i>Aspergillus clavatus</i> produced in a fungal biofilm bioreactor toward <i>Culex quinquefasciatus</i> (Diptera: Culicidae)

et al. 2014

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Many entomopathogenic fungi have been demonstrated to be potential agents for efficiently controlling mosquito populations. In the present study, we investigated a bioreactor system to produce metabolites and conidia by combining technological advantages of submerged and solid-state fermentations. The efficiency of fungal products was tested toward mosquitoes. Aspergillus clavatus (Eurotiales: Trichocomaceae) was grown by semi-solid-state fermentation in a bioreactor for up to 7 days. Depending on conidial doses (2.5×10 7 , 5×10 7 , 7.5×10 7 , 10×10 7 and 12.5×10 7 conidia/mL), mortality ranged from 37.2±15.0 to 86.3±5.0% toward larvae and from 35.8±2.0 to 85.2±1.5% toward adults. The metabolites (10, 20, 40, 60, 80 and 100% v/v) yielded mortality from 23.7±15.0 to 100.0±0.1% toward larvae, and two sprayed volumes (5 and 10 mL) reached 45.5±1.4 and 75.6±2.6% mortality, respectively, toward adults.

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“…• C for different time periods (10,20, 30 and 40 min); then it was cooled to room temperature. The initial moisture content of the medium was adjusted to 64.2 ± 0.26% by adding sterilized distilled water.…”

Section: Substrate Treatmentmentioning

confidence: 99%

Penicillium commune spore production in solid‐state fermentation of coffee pulp at laboratory scale and in a helical ribbons rotating reactor

Nava

Gaime-Perraud

Huerta‐Ochoa

et al. 2006

J of Chemical Tech & Biotech

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Penicillium commune was grown on coffee pulp (CP) by solid-state fermentation (SSF). The effects of the duration of CP thermal treatment and the effects of incubation temperature on spore production yield were studied at laboratory scale. The effect of mixing during fermentation was assayed at pilot plant scale in a 70 L stainless steel non-aseptic reactor equipped with helical ribbons for mixing solids. For thermal treatments of CP at 121• C for 10, 20, 30 and 40 min, no significant difference in spore production yield was observed. Maximum sporulation yield was found at 25• C; when the incubation temperature was higher than 30 • C, the sporulation yield decreased significantly. A spore production yield of 3.7 × 10 9 spores g −1 dry CP was obtained when continuous mixing (0.25 rpm) was used at pilot plant scale; however, a decrease in spore yield (1.4 × 10 9 spores g −1 dry CP) was observed under static conditions. Spore production was not affected when a scale factor between 79 and 105 was assayed from laboratory to pilot plant; at this level, the productivity obtained was 3.1 × 10 7 spores g −1 dry CP h −1 . This value is similar to that found in other reports using natural substrates but working at a smaller scale.

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Characterization of growth and sporulation of Metarhizium anisopliae in solid-substrate fermentation

Cited by 42 publications

References 25 publications

Growth and sporulation stoichiometry and kinetics ofConiothyrium minitans on agar media

Growth and sporulation stoichiometry and kinetics ofConiothyrium minitans on agar media

Pathogenicity of <i>Aspergillus clavatus</i> produced in a fungal biofilm bioreactor toward <i>Culex quinquefasciatus</i> (Diptera: Culicidae)

Penicillium commune spore production in solid‐state fermentation of coffee pulp at laboratory scale and in a helical ribbons rotating reactor

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