Growth and sporulation stoichiometry and kinetics ofConiothyrium minitans on agar media

Ooijkaas, L.P.; Buitelaar, R.M.; Tramper, J.; Rinzema, A.

doi:10.1002/1097-0290(20000805)69:3<292::aid-bit7>3.0.co;2-z

Cited by 22 publications

(9 citation statements)

References 27 publications

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“…Fungal activity leads to losses of C and nutrients from litter by releasing small particles (FPOM) and dissolved compounds from the leaf litter. Fungal production of nutrient-rich spores (Ooijkaas et al, 2000) is also expected to accelerate nutrient losses from decomposing litter. Leaf litter conditioned by fungi is preferentially consumed by detritivores, with consumptions rates being influenced by leaf litter quality (Irons et al, 1988;Schlief and Mutz, 2006;B€ arlocher and Sridhar, 2014) and probably by stoichiometric requirements of the detritivores.…”

Section: Fungal and Bacterial Susceptibility To N Limitationmentioning

confidence: 99%

Ecological stoichiometry of aquatic fungi: current knowledge and perspectives

2016

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Section: Fungal and Bacterial Susceptibility To N Limitationmentioning

confidence: 99%

Ecological stoichiometry of aquatic fungi: current knowledge and perspectives

2016

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“…Therefore, we bracket our estimates with a range of microbial N values. If microbial N content were 5% (Ooijkaas et al 2000), the ingested material would be predicted to be 75 to 90% microbial. If microbes colonizing this leaf litter were 10% N, close to the …”

Section: Variablementioning

confidence: 99%

Selective feeding determines patterns of nutrient release by stream invertebrates

et al. 2014

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One common stoichiometric approach to predicting patterns of nutrient release (excretion + egestion) by animals in aquatic ecosystems is to base predictions on elemental mass-balance constrained by homeostatic maintenance. An easily measured resource composite (i.e., seston, epilithon, or leaf litter) often is used to represent ingested stoichiometry, but whether such a composite is a good indicator of food actually ingested is a relatively unexplored assumption. We examined the application of a stoichiometric model to the diets of 4 generalist stream invertebrates. We fed 3 trichopteran and 1 amphipod taxa rations consisting of cultured algae, stream epilithon, and several species of conditioned leaf litter. The rations ranged widely in C ∶ N from 10 to 69 (molar) and in C ∶ P from 165 to 3500. After a 2-d feeding period, we measured NH 4 + and PO 4 3− excretion, and C, N, and P egestion rates. The relationships observed between the stoichiometries of release and ration were unexpected. Total N ∶ P release rates conformed to stoichiometric predictions for only 1 taxon. Excretion and egestion rates and ratios were generally similar across diets and rarely varied with ration stoichiometry. These patterns were the result of smaller-than-expected responses to leaf-litter rations, which were the most imbalanced relative to body stoichiometry. Analysis of the C ∶ N stoichiometry of foregut material for 2 taxa showed selective ingestion of an N-rich fraction of leaf litter, in 1 case reducing an apparent 8.4 ∶ 1 C ∶ N imbalance between diet and body composition to 1.5 ∶ 1. Our results show that selective feeding can reduce potential stoichiometric imbalances, altering patterns of nutrient release relative to expectations based on bulk-diet stoichiometry. Assuming that stream invertebrates consume materials stoichiometrically similar to a resource composite can obscure understanding of stoichiometric imbalances and the role of invertebrates in nutrient cycles.

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“…Information on the environmental requirements of C. minitans is available (Campbell, 1947;Turner & Tribe, 1976;Su & Leu, 1980;Trutmann et al, 1980;McQuilken et al, 1997c), but very little is known about the nutritiona l requirements of the mycoparasite, with only unde® ned liquid culture media having been investigate d to any extent (Whipps & Gerlagh, 1992;McQuilken et al, 1997 a,c). More recently, considerable eVort has been made into optimising solid-state fermentation of conidial inoculum of C. minitans and the ability to utilize a range of carbon and nitrogen sources, vitamins and trace elements for growth has been identi® ed (Ooijkaas et al, 1998b(Ooijkaas et al, , 1999(Ooijkaas et al, , 2000Weber et al, 1999;Oostra et al, 2000). However, biomass production in these complex systems is diYcult to measure (Ooijkaas et al, 1998a) and no attention has been paid to the production of antifunga l metabolites.…”

Section: Introductionmentioning

confidence: 99%

Some Nutritional Factors Affecting Production of Biomass and Antifungal Metabolites of Coniothyrium minitans

McQuilken¹,

Gemmell²,

Whipps³

2002

Biocontrol Science and Technology

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The ability of the mycoparasit e Coniothyriu m minitans to utilize a range of C and N sources and vitamins for growth, pycnidia l formation and antifunga l metabolite production was examined using a deWned liquid medium. Coniothyriu m minitans was able to use all the C sources tested, with the exception of d-xylose, and all the N sources tested, although growth was generally better on organic N sources rather than NO 3 ± N. Increasing C:N ratios from 9:1± 202:1 with N constant (2.0 g L 2 l l-alanine) resulted in steadily increasing yields, whereas increasing C:N ratios with C constant (40.0 g L 2 l d-glucose) gradually decreased yield. Addition of thiamine to the glucose± alanine basal medium resulted in the greatest increase in growth but biomass was still less than that achieved using an undeWned molasses± yeast medium. Pycnidial production was generally low or failed to occur in the basal medium + C + N sources in the absence of vitamins, but addition of thiamine consistently led to abundan t pycnidial formation. Molasses± yeast static culture provided greater biomass and conidial yields than molasses± yeast shaken culture. Incorporation of C. minitans culture medium into potato dextrose broth (10% v/v) resulted in consistent reduction in growth of Sclerotinia sclerotiorum irrespective of C, N or vitamin content of the basal medium or whether molasses± yeast medium was used. This is the Wrst report of consistent production of antifunga l metabolites by C. minitans.

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Growth and sporulation stoichiometry and kinetics ofConiothyrium minitans on agar media

Cited by 22 publications

References 27 publications

Ecological stoichiometry of aquatic fungi: current knowledge and perspectives

Ecological stoichiometry of aquatic fungi: current knowledge and perspectives

Selective feeding determines patterns of nutrient release by stream invertebrates

Some Nutritional Factors Affecting Production of Biomass and Antifungal Metabolites of Coniothyrium minitans

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