Characterization of Hemoglobin Immobilized in MgAl-Layered Double Hydroxides by the Coprecipitation Method

Abstract

Hemoglobin was immobilized in Mg(2)Al-Layered Double Hydroxides (LDH) by coprecipation method at pH 9.0. Interactions between Hb and LDH particles were investigated by X-ray diffraction patterns, FTIR, UV-vis, circular dichroism, and fluorescence spectroscopies. Morphology and porosity of Mg(2)Al-Hb(cop) biohybrid are analyzed from SEM and TEM images and permeability measurement. The direct electron transfer of immobilized Hb was studied by cyclic voltammetry, and the electrocatalytic activity was evaluated at glassy carbon modified with this Mg(2)Al-Hb(cop) biohybrid. Even though the percentage of electroactive Hb was less than 2%, this bioelectrode showed a low detection limit (1.5 x 10(-8) M) and a very high sensitivity (37 A/M cm(2)) for the amperometric detection of H(2)O(2).

Direct electrochemistry and electrocatalysis of hemoglobin immobilized in layered double hydroxides modified with amino functionalized ionic liquid through coprecipitation technique

“…This value manifested that only those proteins close to electrode surface exchanged electrons with the underlying electrode. However, compared with those of Hb immobilized in protein-gluten films (4.9%) [34] or MgAl-LDH film (1.8%) [16], the electroactive portion of protein had been improved. The results could be ascribed to the 3D porous architecture from the edge-to-edge strong interactions among LDH sheets through AFIL.…”

“…The direct electrochemistry of Hb on SDS-LDH modified electrode was also realized [15]. The direct electrochemical behavior of Hb was investigated at Mg 2 Al-LDH modified electrode [16]. However, the tendency to aggregate and the poor intrinsic conductivity of LDH don't effectively maximize the electron transport rate, which strongly limits its further applications in electrochemistry.…”

Bacteria encapsulated in layered double hydroxides: Towards an efficient bionanohybrid for pollutant degradation

“…These PXRD pattern modifications can be attributed to both a decrease of LDH particle size and an increase of disorder into the layer stacking. For the lowest LDH /ADP ratio, a complete disappearance of the 00l line is observed as for exfoliated LDH phases[41,42] and LDH phases prepared in the presence of enzymes[24,43]which can be attributed to an important dispersion of the layer into the biohybrid. Due to the large ADP cell dimensions (1 x 0.5 x 0.5 m), the intercalation reaction of the whole cells into the layered structure is not expected.Biohybrid formation involving both LDH matrix and bacteria was further confirmed by FTIRanalyses.Fig.…”

“…In the last years, among the wide variety of available supports, Layered Double Hydroxides (LDH) have proved to be highly effective as host structures for biomolecules [16,17] such as amino acids [18][19][20], DNA [21,22], proteins [23][24][25][26] the adsorption or anionic-exchange process [16,17]. The biomolecules entrapped in LDH matrices by such strategy are then protected.…”

“…The biomolecules entrapped in LDH matrices by such strategy are then protected. However such approach has been mainly limited to small biomolecules (amino acid [19], DNA strand [22]) and proteins [23][24][25][26]. To our knowledge, only adsorption process on LDH has been achieved to immobilize whole-cells.…”