Characterization of HLA class I specific antibodies by ELISA using solubilized antigen targets: II. clinical relevance

Zachary, Andrea A.; Ratner, Lloyd E.; Graziani, Julie A; Lucas, Donna P.; Delaney, Nancy; Leffell, Mary S.

doi:10.1016/s0198-8859(00)00253-6

Cited by 58 publications

(33 citation statements)

References 17 publications

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“…В твердофазном иммунолюминесцентном анализе на платформе LUMINEX смесь очищенных антигенов гистосов-местимости иммобилизирована на полистироловых микросферах [11][12][13][14].…”

Section: Introductionunclassified

Comparison of Different Methods of Anti-Hla Antibodies Detection in Diagnosis of Antibody-Mediated Renal Allograft Rejection

Иванова

Столяревич

Гичкун

et al. 2016

RJTAO

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1 ФГБУ «Федеральный научный центр трансплантологии и искусственных органов имени академика В.И. Шумакова» Минздрава России, Москва, Российская Федерация 2 ГБУЗ «Городская клиническая больница № 52 Департамента здравоохранения г. Москвы», Московский городской нефрологический центр, Москва, Российская Федерация 3 Кафедра нефрологии ФПДО ФГБОУ ВО «Московский государственный медико-стоматологический университет им. А.И. Евдокимова», Москва, Российская Федерация Цель исследования: оценка возможности скринингового выявления антител (АТ) к HLA I и II классов в сочетании с морфологическими и иммуногистохимическими методами для диагностики гуморального отторжения трансплантата и сравнение полученных данных в зависимости от применяемого метода оп-ределения АТ к HLA (ELISA и LUMINEX). Материалы и методы. Проанализированы данные 192 паци-ентов с дисфункцией почечного трансплантата, которым была выполнена биопсия трансплантата (c оп-ределением С4d) и исследованы АТ к HLA (у 109 пациентов методом ELISA, у 58 -методом LUMINEX, у 25 пациентов -и ELISA, и LUMINEX). На основании морфологического диагноза пациенты были разделены на 3 группы: 85 пациентов с хроническим отторжением трансплантата (ХОТ), 39 пациентов с острым гуморальным отторжением трансплантата (ОГО), 68 пациентов с иной патологией трансплантата (группа контроля). Результаты. АТ к HLA определялись у 84,7% пациентов с ХОТ, 84,6% пациентов с ОГО и у 33,8% (р < 0,001) в контрольной группе, причем резко доминировали АТ к HLA II класса. Меж-ду уровнем антител, выявляемых различными методами, определялась тесная корреляция (r² для АТ к HLA I класса составил 0,773, для АТ к HLA II класса -0,379, р < 0,01). Однако при применении метода LUMINEX АТ к HLA выявлялись чаще во всех группах, что свидетельствует о большей его чувствитель-ности. Особенно это значимо для диагностики C4d-негативного острого отторжения, частота которого составила 22,2% при LUMINEX vs. 4,8% при использовании метода ELISA. С другой стороны, АТ к HLA методом LUMINEX выявлялись у 55% пациентов контрольной группы, что требовало определения их донорспецифичности. Заключение. При наличии характерной морфологической картины и экспрессии С4d-фрагмента комплемента скрининговое определение АТ к HLA (методами ELISA, LUMINEX) явля-ется достаточным для диагностики гуморального отторжения. Однако в некоторых случаях необходимо применение более специфичных методов диагностики.Ключевые слова: АТ к HLA, С4d-компонент комплемента, антитело-опосредованное отторжение, трансплантационная гломерулопатия.

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Section: Introductionunclassified

Comparison of Different Methods of Anti-Hla Antibodies Detection in Diagnosis of Antibody-Mediated Renal Allograft Rejection

Иванова

Столяревич

Гичкун

et al. 2016

RJTAO

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“…5 Panel Reactive Antibody (PRA or DSA) levels are routinely performed before kidney transplantation. It is a way of measuring the anti-HLA antibodies 6,7,8 in the serum. A person's PRA can be anywhere from 0% to 99%.…”

Section: Introductionmentioning

confidence: 99%

“…A person's PRA can be anywhere from 0% to 99%. PRA represents the percent of the anti-HLA antibodies in recipient serum against a set panel of HLA antigens 6,7,8 and it can identify the risk of hyperacute or vascular rejection.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Hla Mismatch Scoring and the Pre-Transplant Serum Donor Specific Antibody (Dsa) Levels With Histomorphology to Predict the Antibody-Mediated Rejection as Well as the Severity of Rejection

Raghupathy¹,

Arunkumar²,

P³

2016

jemds

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BACKGROUNDImmunologic studies such as Human Leukocyte Antigen (HLA) typing and measurement of the Donor Specific Antibody (DSA) titre are more helpful to assess the long-term success of renal transplantation and in a superior graft survival. Amount of HLA mismatching and the immune response against the graft determine the success of renal transplantation and graft versus host disease. Rejection can be a humoral or T-cell mediated, but the humoral rejection is more important, as it has a very few diagnostic morphological features in the biopsy and these cases are resistant to anti-rejection therapy with a worse prognosis.

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“…However, contemporary adhesive materials do have a major disadvantage, namely that their durability is limited, a limitation which often arises due to their inadequate marginal adaptation. The factors that are most commonly attributed to failure are the harsh conditions of the oral environment, such as temperature change, the fatigue of the bond owing to tooth flexure, the presence of bacterial enzymes, and the aqueous environment [2]. Good marginal adaptation decreases microleakage considerably and also reduces the postoperative sensitivity and the occurrence of secondary caries [3].…”

Section: Introductionmentioning

confidence: 99%

“…The properties of dental composites are highly dependent on the characteristics of the fillers like the shape, size, surface area and porosity. Volumetric change of dental resins has been extensively researched in the literature [1][2][3]. The 2mm layering technique has been the increment thickness of choice in order to overcome the negative effects of volumetric change and the resultant stresses that develop in the bonded surfaces of the tooth structure [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Towards Bioactive Containing Restorative Materials: From Design to Testing In vitro Approach

Perchyonok¹

2015

JDODT

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In any repair of a tooth with permanent restorative materials, the interface is always a sensitive region. The appearance of adhesive materials was a great step forward in dealing with the problems of this region and improving the overall performance of the restorations. However, contemporary adhesive materials do have a major disadvantage, namely that their durability is limited, a limitation which often arises due to their inadequate marginal adaptation. Restorative materials in the new era aim to be "bio-active" and longlasting.As part of our continuous interest in developing the novel bioactive containing restorative materials, we evaluated the effect of the additional bio-actives (such as chitosan, β-carotene, guar gum resin and the combination of the materials) to the commercially available flowable restorative materials such as Premise on the volumetric shrinkage, flexural strength, compressive strength, the surface hardness of the "bio-active" containing composite. Materials and Methods:The bioactive modified restorative materials were prepared by dispersion of the corresponding component with the addition of commercially available flowable restorative material. The surface morphology (SEM) of the freshly prepared restorative materials as well as reactive surface of the material after 24 hours and 3 months storage in artificial saliva has been performed. The physicochemical properties such volumetric shrinkage, Vickers Hardness and flexural strength of the bio-active restorative materials have been measured and are reported. The effect of the bioactive additionto the commercially available flowable restorative materials such as Premise on the shear bond strength of dentin has been evaluated. Results:The SEM images were obtained to characterize the microstructure of the freeze-dried modified restorative material as well as evaluated the durability of the bioactive restorative materials after 24 hours and 3 month exposure to artificial saliva.All bioactive containing flowable restorative materials gave significantly (P< 0.05; non-parametric ANOVA test) higher shear bond values (P< 0.05) than dentin treated or not treated with phosphoric acid.Volumetric changesdue to polymerization in modified composites have been explored using an electronic mercury dilatometer. The average The average for the control Premise samples had a total volumetric change of 2.74%. Guar/Premise had the largest volumetric change from all the modified Premise samples (2.41%). The Chitosan/Premise (1.07%) had the least volumetric change, followed by β−Carotene/ Chitosan/Premise (1.63%) andβ Carotene/Premise (1.75%). The volumetric change starts at different time intervals for all the materials. The Turkey-Kramer multiple comparison test on the rate of shrinkage slopes has confirmed the statements (p<0.05).TheVickers Hardnessof the bio-active composites such as β-carotene/chitosan/ Premise (37.4VH), Guar Gum/Premise (36.2VH), β-carotene/ Premise (36VH), Chitosan/Premise (5.2 VH) versus Premise control (37.6VH) were measured and...

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Characterization of HLA class I specific antibodies by ELISA using solubilized antigen targets: II. clinical relevance

Cited by 58 publications

References 17 publications

Comparison of Different Methods of Anti-Hla Antibodies Detection in Diagnosis of Antibody-Mediated Renal Allograft Rejection

Comparison of Different Methods of Anti-Hla Antibodies Detection in Diagnosis of Antibody-Mediated Renal Allograft Rejection

Comparison of Hla Mismatch Scoring and the Pre-Transplant Serum Donor Specific Antibody (Dsa) Levels With Histomorphology to Predict the Antibody-Mediated Rejection as Well as the Severity of Rejection

Towards Bioactive Containing Restorative Materials: From Design to Testing In vitro Approach

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