2006
DOI: 10.1016/j.cdp.2006.10.007
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Characterization of human breast epithelial cells by confocal Raman microspectroscopy

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Cited by 96 publications
(70 citation statements)
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“…A multi-element 785 nm diode laser (about 20 mW of power) is used for excitation and the Raman spectra of single cell are acquired with the integration time of 20 s from 300 to 3200 cm −1 . Such power and excitation wavelength would not result in any notable cell degeneration [22]. Good signal-to-noise is achieved under these conditions and all the spectra of the same cell lines are averaged to obtain the mean spectra and further used in PCA/LDA analysis.…”
Section: Rs Of Single Cellsmentioning
confidence: 99%
See 1 more Smart Citation
“…A multi-element 785 nm diode laser (about 20 mW of power) is used for excitation and the Raman spectra of single cell are acquired with the integration time of 20 s from 300 to 3200 cm −1 . Such power and excitation wavelength would not result in any notable cell degeneration [22]. Good signal-to-noise is achieved under these conditions and all the spectra of the same cell lines are averaged to obtain the mean spectra and further used in PCA/LDA analysis.…”
Section: Rs Of Single Cellsmentioning
confidence: 99%
“…Differences between normal and abnormal human cell lines (human breast epithelial cells; tumorogenic bone cells, lymphocytes, etc. [1,2,15,22]) have been characterized from Raman spectra profiles by arrays of fingerprint assignments of physical vibration mode or combining with various multivariate statistical analysis methods like principal component analysis (PCA), linear discriminant analysis (LDA), cluster analysis (CA), etc. All of these studies have proved RS is a powerful tool for cancer detection.…”
Section: Introductionmentioning
confidence: 99%
“…In breast cancer research, RS has been applied to distinguish between malignant, normal, and benign breast tissues with significant sensitivity and specificity. [15][16][17][18][19] Several groups reported HER2 amplification assessment using surfaceenhanced RS (SERS). 20,21 However, SERS requires involvement of nanoparticles and antibodies and as such, does not take full advantage of the intrinsic nature of RS.…”
Section: Introductionmentioning
confidence: 99%
“…Raman spectroscopy is a fast, accurate, label-free, and non-destructive analytical tool for the detection of the human cells at the single cell level [10,11]. It can be used to obtain the difference of the intranuclear genetic material between the cancer cells and the normal cells, and the differences of the proteins in the cell membrane and cytoplasm [6,12].…”
Section: Introductionmentioning
confidence: 99%
“…It is known that cellular biochemical components vary depending upon the cancer cells coming from different organs, and different malignancy degrees. The difference is critical for the development of Raman spectroscopy as a new clinical diagnostic approach [10,11,[13][14][15]. The main objective of the present experimental study was to investigate the biochemical difference in these different cancer cells (SH-SY5Y, HeLa, HO-8910, MDA-MB-231, U87, A549), the cells of distinct malignancy degree (MDA-MB-231 and MCF-7) and the normal cell line and cancer cells (HEB and U87) utilizing Raman spectroscopy.…”
Section: Introductionmentioning
confidence: 99%