Characterization of Human Papillomavirus Type 154 and Tissue Tropism of Gammapapillomaviruses

Ure, Agustín Enrique; Forslund, Ola

doi:10.1371/journal.pone.0089342

Cited by 17 publications

(30 citation statements)

References 68 publications

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“…Our observations are not surprising since previous studies have demonstrated that typically cutaneous HPV types (Beta-and Gamma-PVs) were relatively common in the mucosal epithelia, suggesting a possible dual tissue tropism of the majority of HPV species [1,13,32,28,45,46]. In addition, two Gamma-PV types were identified in a surface swab of a genital wart and a cervical cancer biopsy sample, possible as part of the normal mucosal microbiota, as reported previously [23,26].…”

Section: Discussionsupporting

confidence: 88%

Assessing Gammapapillomavirus infections of mucosal epithelia with two broad-spectrum PCR protocols

et al. 2020

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Background: Human papillomaviruses (HPVs) have been divided into mucosal and cutaneous types according to their primary epithelial tissue tropism. However, recent studies showed the presence of several cutaneous types in mucosal lesions and healthy mucosa from different anatomical sites. Methods: Here, the HPV prevalence and type-specific distribution were assessed in a variety of mucosal samples from 435 individuals using a combination of two established broad-spectrum primer systems: Gamma-PV PCR and CUT PCR.Results: Overall HPV prevalence in anal canal swabs, cervical cancer biopsies, genital warts and oral swabs was 85, 47, 62 and 4%, respectively. In anal canal swabs, Alpha-PVs were most frequently found (59%), followed by Gamma-(37%) and Beta-PVs (4%). The prevalence and persistence of HPV infection in the anal canal of 226 individuals were further explored. Overall HPV, Gamma-PVs and multiple HPV infections were significantly higher in men vs. women (p = 0.034, p = 0.027 and p = 0.003, respectively); multiple HPV infections were more common in individuals ≤40 years (p = 0.05), and significantly higher prevalence of Gamma-PVs and multiple HPV infections was observed in HIV-1-positive vs. HIV-1-negative individuals (p = 0.003 and p = 0.04, respectively). Out of 21 patients with follow-up anal swabs, only one persistent infection with the same type (HPV58) was detected. Conclusions: Our findings suggest that Gamma-PVs (except species Gamma-6) are ubiquitous viruses with dual muco-cutaneous tissue tropism. Anal canal Gamma-PV infections may be associated with sexual behavior and the host immune status. This study expands the knowledge on Gamma-PVs' tissue tropism, providing valuable data on the characteristics of HPV infection in the anal canal.

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Section: Discussionsupporting

confidence: 88%

Assessing Gammapapillomavirus infections of mucosal epithelia with two broad-spectrum PCR protocols

et al. 2020

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“…In contrast, because more than 90% of warts identified in the anogenital region of adult patients are etiologically associated with sexually transmitted HPV6 and HPV11 (11,12,(16)(17)(18)43), when testing this patient population the method of choice in our laboratory is the HPV6/11 RT-PCR (40), followed in the case of a negative result by HPV2/27/57 multiplex RT-PCR and Low-risk Alpha-PV-PCR (31). Alpha-PV-negative wart tissue samples are additionally tested in our laboratory for research purposes only for the presence of several Gamma-and Mu-PVs that cause sporadic cutaneous warts (7,9,11,12,26,44,45). All HPV-negative warts identified in the anogenital region of patients of all ages and both sexes are additionally tested in our laboratory for the presence of molluscum contagiosum virus (MCV) using the MCV FRET RT-PCR (45) because, due to the similar clinical appearance of lesions, up to 10% of molluscum contagiosum lesions can be misdiagnosed as condylomata acuminata or verrucae vulgares and vice versa (45)(46)(47)(48).…”

Section: Discussionmentioning

confidence: 99%

Development of a novel multiplex type-specific quantitative real-time PCR for detection and differentiation of infections with human papillomavirus types HPV2, HPV27, and HPV57

Hošnjak

Komloš

Kocjan

et al. 2016

Acta Dermatovenerologica Alpina Pannonica Et Adriatica

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Introduction:The present study describes the development and evaluation of the first multiplex type-specific quantitative realtime PCR (RT-PCR), enabling simple, rapid, sensitive, and specific concurrent detection and differentiation of human papillomavirus (HPV) types HPV2, 27, and 57 in a single PCR reaction. Results: The HPV2/27/57 multiplex RT-PCR with a dynamic range of seven orders of magnitude (discriminating 10 to 10 8 viral genome equivalents/reaction) has an analytical sensitivity of at least 10 viral copies of each targeted HPV type/reaction, and no cross-reactivities were observed among the included targets. All three primer/probe combinations were efficient in amplifying 500 copies of targeted DNA in a background of 10 8 , 10 7 , 500, 100, and 10 copies of non-targeted viral DNA/reaction, and the performance of the HPV2/27/57 multiplex RT-PCR was additionally not affected by the presence of background human genomic DNA. When testing DNA isolates obtained from fresh-frozen tissue specimens of various children's warts, the results of the HPV2/27/57 multiplex RT-PCR were completely in line with the results of the conventional Low-risk Alpha-PV PCR. Conclusion: The newly developed HPV2/27/57 multiplex RT-PCR is an appropriate test for use in routine clinical laboratory settings and for studies focusing on the molecular epidemiology, pathogenesis, and natural history of HPV2/27/57-related lesions.

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“…HPV154 species γ-11, was first identified by FAP-PCR from a swab of a wart at the intergluteal cleft of a three-year-old boy [67]. The complete genome was obtained by combination of RCA, genome walking through an adapted method for detection of integrated PV sequences by ligation-mediated PCR (DIPS-PCR), long-range PCR and, finally, by cloning of four overlapping amplicons of RCA-amplified material.…”

Section: Hpv154mentioning

confidence: 99%

“…The complete genome was obtained by combination of RCA, genome walking through an adapted method for detection of integrated PV sequences by ligation-mediated PCR (DIPS-PCR), long-range PCR and, finally, by cloning of four overlapping amplicons of RCA-amplified material. HPV154 has been detected in 3% (2/62) of forehead skin swabs from healthy children [67].…”

Section: Hpv154mentioning

confidence: 99%

Molecular methods for identification and characterization of novel papillomaviruses

Kocjan

Bzhalava

Forslund

et al. 2015

Clinical Microbiology and Infection

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Papillomaviruses (PV) are a remarkably heterogeneous family of small DNA viruses that infect a wide variety of vertebrate species and are aetiologically linked with the development of various neoplastic changes of the skin and mucosal epithelia. Based on nucleotide similarity, PVs are hierarchically classified into genera, species and types. Novel human PV (HPV) types are given a unique number only after the whole genome has been cloned and deposited with the International HPV Reference Center. As of 9 March 2015, 200 different HPV types, belonging to 49 species, had been recognized by the International HPV Reference Center. In addition, 131 animal PV types identified from 66 different animal species exist. Recent advances in molecular techniques have resulted in an explosive increase in the identification of novel HPV types and novel subgenomic HPV sequences in the last few years. Among PV genera, the γ-PV genus has been growing most rapidly in recent years with 80 completely sequenced HPV types, followed by α-PV and β-PV genera that have 65 and 51 recognized HPV types, respectively. We reviewed in detail the contemporary molecular methods most often used for identification and characterization of novel PV types, including PCR, rolling circle amplification and next-generation sequencing. Furthermore, we present a short overview of 12 and 10 novel HPV types recently identified in Sweden and Slovenia, respectively. Finally, an update on the International Human Papillomavirus Reference Center is provided.

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Characterization of Human Papillomavirus Type 154 and Tissue Tropism of Gammapapillomaviruses

Cited by 17 publications

References 68 publications

Assessing Gammapapillomavirus infections of mucosal epithelia with two broad-spectrum PCR protocols

Assessing Gammapapillomavirus infections of mucosal epithelia with two broad-spectrum PCR protocols

Development of a novel multiplex type-specific quantitative real-time PCR for detection and differentiation of infections with human papillomavirus types HPV2, HPV27, and HPV57

Molecular methods for identification and characterization of novel papillomaviruses

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