Characterization of hybrid seedlings from crosses of &lt;i&gt;Nicotiana stocktonii&lt;/i&gt; Brandegee×&lt;i&gt;N. tabacum&lt;/i&gt; L. and &lt;i&gt;N. stocktonii&lt;/i&gt;×progenitors of &lt;i&gt;N. tabacum&lt;/i&gt;

Muraida, Naho; Marubashi, Wataru

doi:10.5511/plantbiotechnology.15.0402a

Cited by 3 publications

(2 citation statements)

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“…N. tabacum (2n = 4x = 48, genome SSTT) has an S subgenome derived from N. sylvestris (2n = 2x = 24) and a T subgenome derived from N. tomentosiformis (2n = 2x = 24), the progenitors of tobacco. Hybrid seedlings from the N. stocktonii × N. tabacum cross have been successfully rescued through ovule culture [110], as they did not highlight lethality. The flowers of N. stocktonii, used as the female parent, were emasculated and fertilized with N. tabacum pollen.…”

Section: Dicot Speciesmentioning

confidence: 99%

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Embryo Rescue in Plant Breeding

Rogo,

Fambrini,

Pugliesi

2023

Plants

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Embryo rescue (ER) techniques are among the oldest and most successful in vitro tissue culture protocols used with plant species. ER refers to a series of methods that promote the development of an immature or lethal embryo into a viable plant. Intraspecific, interspecific, or intergeneric crosses allow the introgression of important alleles of agricultural interest from wild species, such as resistance or tolerance to abiotic and biotic stresses or morphological traits in crops. However, pre-zygotic and post-zygotic reproductive barriers often present challenges in achieving successful hybridization. Pre-zygotic barriers manifest as incompatibility reactions that hinder pollen germination, pollen tube growth, or penetration into the ovule occurring in various tissues, such as the stigma, style, or ovary. To overcome these barriers, several strategies are employed, including cut-style or graft-on-style techniques, the utilization of mixed pollen from distinct species, placenta pollination, and in vitro ovule pollination. On the other hand, post-zygotic barriers act at different tissues and stages ranging from early embryo development to the subsequent growth and reproduction of the offspring. Many crosses among different genera result in embryo abortion due to the failure of endosperm development. In such cases, ER techniques are needed to rescue these hybrids. ER holds great promise for not only facilitating successful crosses but also for obtaining haploids, doubled haploids, and manipulating the ploidy levels for chromosome engineering by monosomic and disomic addition as well substitution lines. Furthermore, ER can be used to shorten the reproductive cycle and for the propagation of rare plants. Additionally, it has been repeatedly used to study the stages of embryonic development, especially in embryo-lethal mutants. The most widely used ER procedure is the culture of immature embryos taken and placed directly on culture media. In certain cases, the in vitro culture of ovule, ovaries or placentas enables the successful development of young embryos from the zygote stage to maturity.

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Section: Dicot Speciesmentioning

confidence: 99%

“…These results suggested that both S and T genomes harbor factors causing hybrid lethality in N. stocktonii. Muraida and Marubashi [110] proposed that in N. tabacum, the factors responsible for lethality must have been lost due to the reorganization and modification of the genome during and after amphidiploid formation.…”

Section: Dicot Speciesmentioning

confidence: 99%