Characterization of <i>Klebsiella pneumoniae</i> Coproducing KPC and NDM-1 Carbapenemases from Turkey

Tekelı, Alper; Dolapçı, İştar; Evren, Ebru; Oguzman, Elif; Karahan, Zeynep Ceren

doi:10.1089/mdr.2019.0086

Cited by 25 publications

(17 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, first KPC-2-positive K. pneumoniae have been identified in Turkey, in 2014 [31]. Since then, sporadic KPC-producing K. pneumoniae was reported [32,33]. In the present study, we didn't detect bla KPC gene among tested carbapenem and colistin resistant K. pneumoniae isolates.…”

Section: Discussioncontrasting

confidence: 65%

“…A single K. pneumoniae clone ST258 was identified extensively worldwide, indicating that it may have contributed to the spread of the bla KPC genes [26]. On the other hand, KPC-producing K. pneumoniae remains rare for our country [31,32]. Among all tested isolates, we didn't detect KPC-producing K. pneumoniae and also its emerging high-risk clone ST258.…”

Section: Discussionmentioning

confidence: 70%

“…OXA-48-producing K. pneumoniae was first reported from Turkey and it is endemic for our country [30,32,36,37]. The emergence of the OXA-48 enzyme is mediated by the rapid spread of a broad host-range conjugative plasmid harboring the blaOXA-48 gene.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Emergence of carbapenemase-producing and colistin resistant Klebsiella pneumoniae ST101 high-risk clone in Turkey

Hazırolan

Karagöz

2020

AMicr

View full text Add to dashboard Cite

Carbapenemase-producing and colistin resistant Klebsiella pneumoniae has become a worldwide healthcare problem. This study describes molecular characterization of carbapenemase-producing and colistin resistant clinical K. pneumoniae isolates.A total of 93 non-replicate carbapenem and colistin resistant K. pneumoniae were recovered from clinical specimens in a university hospital during 2017–2019. Detection of blaOXA-48, blaKPC, blaNDM-1, blaIMP, blaVIM-1 and mcr-1, -2, -3, -4, -5, -6, -7, and -8 genes was performed by PCR. The bacterial isolates were assigned to clonal lineages by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST).All isolates harbored blaOXA-48 and only two isolates harbored blaOXA-48, and blaNDM-1 genes together. In colistin resistant K. pneumoniae, mcr-1 was detected in two (2.1%) isolates. Ninety three isolates of K. pneumoniae were categorized into three clusters and five pulsotypes. MLST revealed two different sequence types, ST101 (89/93) and ST147 (4/93).In our study ST101 was found to be a significantly dominant clone carrying blaOXA-48 and among our strains a low frequency of mcr-1 gene was determined. The emergence of colistin resistance was observed in K. pneumoniae ST101 isolates. ST101 may become a global threat in the dissemination of carbapenem and colistin resistance.

show abstract

Section: Discussioncontrasting

confidence: 65%

Section: Discussionmentioning

confidence: 70%

See 1 more Smart Citation

Emergence of carbapenemase-producing and colistin resistant Klebsiella pneumoniae ST101 high-risk clone in Turkey

Hazırolan

Karagöz

2020

AMicr

View full text Add to dashboard Cite

show abstract

“…In Iran, blaVIM and blaIMP genes frequencies were 15.6% and 6.4% respectively (Khodadadian et al, 2018). Tekeli et al (2020) reported that all isolates were positive for NDM‐1 , in Turkey. Whereas 10 (21.73%) isolates were found to be positive for NDM‐1 in Egypt (Khalil et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Biotyping, virulotyping and biofilm formation ability of ESBL-Klebsiella pneumoniae isolates from nosocomial infections

Ahmed

Ibrahim

Abdelhaliem

et al. 2022

Journal of Applied Microbiology

View full text Add to dashboard Cite

The aim of this study was to investigate the frequency, molecular characterization, virulence genes, resistance genes and antimicrobial profile of nosocomial extended spectrum beta lactamase producing Klebsiella species. A total of 22 (12.2%) K. pneumoniae strains were isolated from 180 clinical samples collected from hospitalized patients in Egypt. K. pneumoniae biotypes were B1 (72.8%), B3 (13.6%) and B4 (13.6%). The isolates were classified for the capsular serotypes, 86.4% (20/22) were of K1 serotype, while only two isolates (13.64%) were of K2 serotype. Hypermucoviscous K. pneumoniae isolates accounted for 68.2%. Biofilm formation ability of K. pneumoniae was determined by microtitre plate method. The majority of the isolates (40.9%) were moderate biofilm producers, while 27.3% were strong biofilm producers. All K. pneumoniae strains were positive for fimH and traT genes, while magA was identified in only 63.6% of the isolates. The antibiotic susceptibility profile of the isolates (n = 22) was determined by the disc diffusion technique using 23 different antibiotics. Streptomycin and imipenem are the most effective antibiotics against 22 tested K. pneumoniae isolates with sensitivity rates of 63.64% and 54.54% respectively. All tested K. pneumoniae isolates showed high resistance to amoxicillin∕clavulanate (100%), cefuroxime (100%) and ceftazidime (95.45%). Extended spectrum beta lactamases (ESBL) production and the presence of ESBL‐related genes were tested in the isolates. All the isolates tested positive for blaVIM, NDM1 and blaTEM, while only 81.8 %tested positive for the blaSHV gene. Increasing antimicrobial resistance in K. pneumoniae causing nosocomial infections limits the use of antimicrobial agents for treatment. Furthermore, the spread of biofilm, multiple drug resistant and ESBL‐producing K. pneumoniae isolates is a public threat for hospitalized patients.

show abstract

“…Worryingly, the co-production and accumulation of genetic resistance determinants have become typically reported among highly resistant K. pneumoniae isolates. Certainly, most of the NDM-producing isolates co-harbor a broad variety of resistance mechanisms, such as CTX-M, SHV, KPC, VIM, and OXA-48, highlighting the increasing incidence of bla NDM and bla KPC co-producing strains, as reported in China, USA, Greece, India, Pakistan and Turkey [ 7 , 25 , 43 , 44 , 45 , 46 , 47 , 48 ]. In April 2021, Argentina’s National Antimicrobial Reference Laboratory was alerted to the emergence and spread of Enterobacterales producing different combinations of carbapenemases, especially during the first wave of the COVID-19 pandemic.…”

Section: Discussionmentioning

confidence: 99%

The Spread of NDM-1 and NDM-7-Producing Klebsiella pneumoniae Is Driven by Multiclonal Expansion of High-Risk Clones in Healthcare Institutions in the State of Pará, Brazilian Amazon Region

et al. 2021

View full text Add to dashboard Cite

Carbapenem resistance among Klebsiella pneumoniae isolates is often related to carbapenemase genes, located in genetic transmissible elements, particularly the blaKPC gene, which variants are spread in several countries. Recently, reports of K. pneumoniae isolates harboring the blaNDM gene have increased dramatically along with the dissemination of epidemic high-risk clones (HRCs). In the present study, we report the multiclonal spread of New Delhi metallo-beta-lactamase (NDM)-producing K. pneumoniae in different healthcare institutions in the state of Pará, Northern Brazil. A total of 23 NDM-producing isolates were tested regarding antimicrobial susceptibility testing features, screening of carbapenemase genes, and genotyping by multilocus sequencing typing (MLST). All K. pneumoniae isolates were determined as multidrug-resistant (MDR), being mainly resistant to carbapenems, cephalosporins, and fluoroquinolones. The blaNDM-7 (60.9%—14/23) and blaNDM-1 (34.8%—8/23) variants were detected. MLST genotyping revealed the predomination of HRCs, including ST11/CC258, ST340/CC258, ST15/CC15, ST392/CC147, among others. To conclude, the present study reveals the contribution of HRCs and non-HRCs in the spread of NDM-1 and NDM-7-producing K. pneumoniae isolates in Northern (Amazon region) Brazil, along with the first detection of NDM-7 variant in Latin America and Brazil, highlighting the need for surveillance and control of strains that may negatively impact healthcare and antimicrobial resistance.

show abstract

Characterization of Klebsiella pneumoniae Coproducing KPC and NDM-1 Carbapenemases from Turkey

Cited by 25 publications

References 48 publications

Emergence of carbapenemase-producing and colistin resistant Klebsiella pneumoniae ST101 high-risk clone in Turkey

Emergence of carbapenemase-producing and colistin resistant Klebsiella pneumoniae ST101 high-risk clone in Turkey

Biotyping, virulotyping and biofilm formation ability of ESBL-Klebsiella pneumoniae isolates from nosocomial infections

The Spread of NDM-1 and NDM-7-Producing Klebsiella pneumoniae Is Driven by Multiclonal Expansion of High-Risk Clones in Healthcare Institutions in the State of Pará, Brazilian Amazon Region

Contact Info

Product

Resources

About