Characterization of igf1 and igf2 genes during maraena whitefish ( Coregonus maraena ) ontogeny and the effect of temperature on embryogenesis and igf expression

Nipkow, Mareen; Wirthgen, Elisa; Luft, Peter; Rebl, Alexander; Hoeflich, Andreas; Goldammer, Tom

doi:10.1016/j.ghir.2018.04.003

Cited by 19 publications

(8 citation statements)

References 54 publications

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“…The growth process in fish is regulated through the growth hormone synthesized in hypophysis and then it is released into the bloodstream. The GH protein will interact with its specific receptors on the cells and altered the intracellular-somatotrophic signaling pathways, with insulin-like growth factor 1 (IGF-1) as the main mediator for cell proliferation and growth (Fuentes et al, 2013;Nipkow et al, 2018)…”

Section: Resultsmentioning

confidence: 99%

Gene Transmission, Growth, and Exogeneous Growth Hormone Expression of G2 Transgenic Betta Fish (Betta imbellis)

Ayuningthias

Nasrullah

Soelistyowati

et al. 2021

JIPK

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Highlight ResearchThe F2 of GH-transgenic B. imbellis was successfully producedThe transgene inheritance by the F2 fish was more than 90%The growth and body size of transgenic fish was significantly higher than controlF2 fish reached a larger body size in a shorter period compared to the F1 AbstractIn our previous research, we had successfully produced G0 and G1 Pangasianodon hypophthalmus growth hormone (PhGH) transgenic B. imbellis, native ornamental betta from Indonesia, which its giant-sized variant has valuable price for the breeders. The G0 and G1 transgenic (TG) fish showed higher growth rate and body size compared to the non-transgenic (NT) fish. The study was aimed to produce and evaluate the consistencies of transgene transmission and expression in G2 generation. The growth rate and body size between TG and NT fish was also compared. The G2 generation was produced using crosses between TG and NT G1 fish: ♂TG × ♀TG, ♂TG × ♀NT, ♂NT × ♀TG, and ♂NT ×♀ NT. Fish were reared for 12 weeks, and transgene detection was performed using the polymerase chain reaction method (PCR) on isolated DNA from the caudal fin clips. The endogenous and exogenous GH expression analysis was conducted using the quantitative real-time PCR (qPCR) method. The results showed that the inheritance of the GH transgene by the G2 fish was more than 90% in all transgenic crosses. Endogenous GH was expressed at the same levels in the brain of TG and NT fish, but the exogenous GH expression was highly detected only in the TG fish. The G2 transgenic fish had a higher specific growth rate, up to 31%, compared to the control. The body length of TG crosses were 23−35% higher and had 111−135% higher body weight compared to NT fish. These results showed a promising approached in mass-producing stable lines of giant-sized betta using the GH-transgenic technology.

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Section: Resultsmentioning

confidence: 99%

Gene Transmission, Growth, and Exogeneous Growth Hormone Expression of G2 Transgenic Betta Fish (Betta imbellis)

Ayuningthias

Nasrullah

Soelistyowati

et al. 2021

JIPK

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“…Investigations of gilthead sea bream ( Sparus aurata ) demonstrated a positive correlation between the expression of GHR and a growth spurt during summer months (Calduch-Giner et al 2003 ). Nipkow et al ( 2018 ) detected increased IGF2 transcript levels in maraena whitefish ( Coregonus maraena ) at the onset of oral feeding and during development into fingerlings. We found similar patterns in pikeperch with an increase in IGF2 and GHR transcript levels from 0 dph to 4 and 7 dph and the highest levels at 121 dph, reflecting the strong phase of growth of fingerlings.…”

Section: Discussionmentioning

confidence: 99%

“…To establish a screening panel for key steps of the developmental process, we included 21 genes involved in stress ( NR3C1 , endothelial PAS domain protein 1 [ EPAS1 ], hypoxia inducible factor 1 subunit alpha [ HIF1A ], heat shock transcription factor 1 [ HSF1 ], heat shock transcription factor 2 [ HSF2 ], teleost-specific osmotic stress transcription factor 1 [ tOSTF1 ]; Le Goff et al 2004 ; Deane and Woo 2011 ; Tse 2014 ; Malandrakis et al 2016 ; Pelster and Egg 2018 ), and immune response (interleukin 1 beta [ IL1B ], lysozyme [ LYZ ]; Saurabh and Sahoo 2008 ; Zou and Secombes 2016 ) as well as cell homeostasis (transcription factor EB [ TFEB ]; Settembre and Ballabio 2011 ; Raben and Puertollano 2016 ), nutritional status ( APOE , PPARA , PPARD ; Poupard et al 2000 ; Leaver et al 2005 ; Napolitano and Ballabio 2016 ), growth (insulin-like growth factor 2 [ IGF2 ], GHR ; Bergan-Roller and Sheridan 2018 ; Nipkow et al 2018 ), energy metabolism ( CKM , glycine amidinotransferase [ GATM ]; Borchel et al 2019 ), the process of gonadal maturation ( SOX9 ; Leet et al 2011 ; Bhat et al 2016 ), and the organogenesis of the early life stages ( BMP4 , BMP7 , myosin heavy chain [ MYH6 ], RXRA ; He et al 2009 ; Ahi 2016 ; Bloomquist et al 2017 ; Tang et al 2018 ) (Table 1 ). For the stress screening of SLUlar1 and WF2 cells, the additional immune markers interleukin 8 ( CXCL8 ) and interleukin 10 ( IL10 ) were included.…”

Section: Methodsmentioning

confidence: 99%

Insights into early ontogenesis: characterization of stress and development key genes of pikeperch (Sander lucioperca) in vivo and in vitro

Schäfer

Kaya

Rebl

et al. 2021

Fish Physiol Biochem

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There are still numerous difficulties in the successful farming of pikeperch in the anthropogenic environment of various aquaculture systems, especially during early developmental steps in the hatchery. To investigate the physiological processes involved on the molecular level, we determined the basal expression patterns of 21 genes involved in stress and immune responses and early ontogenesis of pikeperch between 0 and 175 days post hatch (dph). Their transcription patterns most likely reflect the challenges of growth and feed conversion. The gene coding for apolipoprotein A (APOE) was strongly expressed at 0 dph, indicating its importance for yolk sac utilization. Genes encoding bone morphogenetic proteins 4 and 7 (BMP4, BMP7), creatine kinase M (CKM), and SRY-box transcription factor 9 (SOX9) were highly abundant during the peak phases of morphological changes and acclimatization processes at 4–18 dph. The high expression of genes coding for peroxisome proliferator-activated receptors alpha and delta (PPARA, PPARD) at 121 and 175 dph, respectively, suggests their importance during this strong growth phase of juvenile stages. As an alternative experimental model to replace further in vivo investigations of ontogenetically important processes, we initiated the first approach towards a long-lasting primary cell culture from whole pikeperch embryos. The present study provides a set of possible biomarkers to support the monitoring of pikeperch farming and provides a first basis for the establishment of a suitable cell model of this emerging aquaculture species.

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“…In CMAfin1 cells of P7, P19, P34, and P37, we investigated the expression of the 10 genes selected as epithelial cell markers (cytokeratin 8, KRT8 ; cadherin‐1, CDH1 ), fibroblast (vimentin, VIM ), mesenchymal (collagen type I alpha 1 chain, COL1A1 ), proliferation (proliferating cell nuclear antigen, PCNA ) cytoskeleton (actin beta, ACTB ) and stem cell marker (SRY‐box transcription factor 2, SOX2 ; SRY‐box transcription factor 9, SOX9 ; insulin‐like growth factors 1, IGF1 , and insulin‐like growth factor 2, IGF2 ). No cDNA sequences were publicly available for the selected marker genes at the beginning of the study, except for IGF1 , and − 2 , ACTB , and the chosen reference genes eukaryotic translation elongation factor 1 alpha 1, variant A ( EEF1A1A ), ribosomal protein L9 ( RPL9 ) and –L32 ( RPL32 ) (Nipkow et al, 2018). Therefore, we identified orthologous gene sequences from the closely related salmonid species Oncorhynchus mykiss from the NCBI (National Center for Biotechnology Information) GenBank (GB) database and used BLAST searches against the published transcriptome of C. maraena (RefSeq NCBI BioProjekt: PRJNA302355) to identify corresponding sequence fragments.…”

Section: Methodsmentioning

confidence: 99%

Establishment of an in vitro model from the vulnerable fish species Coregonus maraena (maraena whitefish): Optimization of growth conditions and characterization of the cell line

Kaya

Tönißen

Verleih

et al. 2022

Cell Biology International

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In this study, a cell line of the fish species Coregonus maraena was produced for the first time. C. maraena is an endangered species, and studies indicate that this fish species will be affected by further population declines due to climate change. This cell line, designated CMAfin1, has been maintained in Leibovitz L‐15 supplemented with 10% fetal bovine serum over 3 years. Both subculturing and storage (short‐term storage at −80°C and long‐term storage in liquid nitrogen) was successful. Cell morphology and growth rate were consistent from passage 10 onwards. Immunocytochemical examination of cellular proteins and matrix components confirmed the mechanical stability of the cells. Actin, fibronectin, vinculin, vimentin, and tubulin are present in the cells and form a network. In addition, the transport of molecules is ensured by the necessary proteins. Gene expression analysis showed a shift in the expressions of stem cell markers between younger and higher passages. While SOX2 and IGF1 were more highly expressed in the seventh passage, SOX9 and IGF2 expressions were significantly increased in higher passages. Therefore, the stable cell culture CMAfin1 can be used for applied analysis to further understand the cell physiology of C. maranea.

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Characterization of igf1 and igf2 genes during maraena whitefish ( Coregonus maraena ) ontogeny and the effect of temperature on embryogenesis and igf expression

Cited by 19 publications

References 54 publications

Gene Transmission, Growth, and Exogeneous Growth Hormone Expression of G2 Transgenic Betta Fish (Betta imbellis)

Gene Transmission, Growth, and Exogeneous Growth Hormone Expression of G2 Transgenic Betta Fish (Betta imbellis)

Insights into early ontogenesis: characterization of stress and development key genes of pikeperch (Sander lucioperca) in vivo and in vitro

Establishment of an in vitro model from the vulnerable fish species Coregonus maraena (maraena whitefish): Optimization of growth conditions and characterization of the cell line

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