Characterization of intact microorganisms by MALDI mass spectrometry

Fenselau, Catherine; Demirev, Plamen A.

doi:10.1002/mas.10004

Cited by 745 publications

(257 citation statements)

References 100 publications

Supporting

Mentioning

243

Contrasting

Unclassified

Order By: Relevance

“…Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) is a rapid, accurate, sensitive, and cost-effective method that offers an adequate alternative to genome-based approaches and that has been widely used for identification and typing of microorganisms in a clinical routine setup (12)(13)(14)(15)(16)(17)(18)(19), as well as for HPB (20)(21)(22)(23)(24)(25)(26)(27). This method does not depend on exclusive consumables and has revealed high levels of reproducibility in both intralaboratory and interlaboratory studies (28,29).…”

mentioning

confidence: 99%

Identification of Highly Pathogenic Microorganisms by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Results of an Interlaboratory Ring Trial

et al. 2015

View full text Add to dashboard Cite

mIn the case of a release of highly pathogenic bacteria (HPB), there is an urgent need for rapid, accurate, and reliable diagnostics. MALDI-TOF mass spectrometry is a rapid, accurate, and relatively inexpensive technique that is becoming increasingly important in microbiological diagnostics to complement classical microbiology, PCR, and genotyping of HPB. In the present study, the results of a joint exercise with 11 partner institutions from nine European countries are presented. In this exercise, 10 distinct microbial samples, among them five HPB, Bacillus anthracis, Brucella canis, Burkholderia mallei, Burkholderia pseudomallei, and Yersinia pestis, were characterized under blinded conditions. Microbial strains were inactivated by high-dose gamma irradiation before shipment. Preparatory investigations ensured that this type of inactivation induced only subtle spectral changes with negligible influence on the quality of the diagnosis. Furthermore, pilot tests on nonpathogenic strains were systematically conducted to ensure the suitability of sample preparation and to optimize and standardize the workflow for microbial identification. The analysis of the microbial mass spectra was carried out by the individual laboratories on the basis of spectral libraries available on site. All mass spectra were also tested against an in-house HPB library at the Robert Koch Institute (RKI). The averaged identification accuracy was 77% in the first case and improved to >93% when the spectral diagnoses were obtained on the basis of the RKI library. The compilation of complete and comprehensive databases with spectra from a broad strain collection is therefore considered of paramount importance for accurate microbial identification. Highly pathogenic bacteria (HPB) are risk group 3 bacteria, which are defined as biological agents that can cause severe human disease and present a serious hazard to health care workers. This may present a risk of spreading to the community, but there is usually effective prophylaxis or treatment available (1). To this group belong bacteria such as Bacillus anthracis, Francisella tularensis subsp. tularensis (type A), Yersinia pestis, species of the Brucella melitensis group, Burkholderia mallei, and Burkholderia pseudomallei. HPB have the potential to be used in bioterrorist attacks (2, 3). The Centers for Disease Control and Prevention (CDC, Atlanta, GA) have classified B. anthracis, F. tularensis, and Y. pestis as category A and Brucella species, B. mallei, B. pseudomallei, and Coxiella burnetii as category B, comprising the main pathogens of concern for use in bioterrorist attacks (4). These pathogens may cause anthrax, tularemia, plague, brucellosis, glanders, melioidosis, and Q fever, respectively. In most parts of the world, the natural prevalence of these agents is low, even though some of these agents cause outbreaks in human and animal populations from time to time (5-8). The intentional release of these agents, however, can result in severe public health consequences, as was shown in the U...

show abstract

mentioning

confidence: 99%

Identification of Highly Pathogenic Microorganisms by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Results of an Interlaboratory Ring Trial

et al. 2015

View full text Add to dashboard Cite

show abstract

“…During IC/IS MALDI-TOF MS, unique peptide/proteins are desorbed and ionized from the analyzed species and profile mass spectra are acquired [9]. After a great success, which had accompanied MALDI-based bacterial identification since its introduction [10], numerous protocols were optimized for microscopic fungi [11,12]. The procedure of sample preparation including the choice of matrix, solvents and sample deposition technique (namely with respect to the homogeneity of sample-matrix co-crystals) is the most critical part [12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…MALDI-TOF mass spectra are acquired in the m/z range of 2000 to 20,000 and searched against a database of characteristic protein profiles [18]. Because of a significant salt and detergent tolerance, fast and accurate molecular mass determination, and the possibility of automation, MALDI-TOF MS has been established as a powerful tool for microbial identification [10].…”

Section: Introductionmentioning

confidence: 99%

Intact spore MALDI-TOF mass spectrometry and proteomic analysis of Puccinia pathogenic fungi

Beinhauer

Raus

Hanzalová

et al. 2016

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

View full text Add to dashboard Cite

“…Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been widely demonstrated to be an excellent tool for the rapid, accurate, and inexpensive identification of microorganisms (7,8). Groups are now investigating this technology for potentially equally rapid, simple, and inexpensive antimicrobial Citation Gitman MR, McTaggart L, Spinato J, Poopalarajah R, Lister E, Husain S, Kus JV.…”

mentioning

confidence: 99%

Antifungal Susceptibility Testing of Aspergillus spp. by Using a Composite Correlation Index (CCI)-Based Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Method Appears To Not Offer Benefit over Traditional Broth Microdilution Testing

et al. 2017

View full text Add to dashboard Cite

Aspergillus spp. cause serious invasive lung infections, and Aspergillus fumigatus is the most commonly encountered clinically significant species. Voriconazole is considered to be the drug of choice for treating A. fumigatus infections; however, rising resistance rates have been reported. We evaluated a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based method for the differentiation between wild-type and non-wild-type isolates of 20 Aspergillus spp. (including 2 isolates of Aspergillus ustus and 1 of Aspergillus calidoustus that were used as controls due their intrinsic low azole susceptibility with respect to the in vitro response to voriconazole). At 30 and 48 h of incubation, there was complete agreement between Cyp51A sequence analysis, broth microdilution, and MALDI-TOF MS classification of isolates as wild type or non-wild type. In this proof-of-concept study, we demonstrated that MALDI-TOF MS can be used to accurately detect A. fumigatus strains with reduced voriconazole susceptibility. However, rather than proving to be a rapid and simple method for antifungal susceptibility testing, this particular MS-based method showed no benefit over conventional testing methods.KEYWORDS Aspergillus, voriconazole resistance, MALDI-TOF MS, antifungal susceptibility testing, composite correlation index, voriconazole A spergillus spp. cause serious invasive lung infections, and Aspergillus fumigatus is the most commonly encountered clinically significant species (1). Voriconazole is considered to be the drug of choice for treating A. fumigatus infections (2); however, rising resistance rates have been reported (3, 4), and antifungal susceptibility testing (AFST) remains limited to a small number of specialized laboratories. Early initiation of appropriate therapy has been shown to lead to better clinical outcomes (5). Additionally, it has been demonstrated that patients infected with resistant strains have poorer outcomes than those infected with susceptible strains (6).Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been widely demonstrated to be an excellent tool for the rapid, accurate, and inexpensive identification of microorganisms (7,8). Groups are now investigating this technology for potentially equally rapid, simple, and inexpensive antimicrobial Citation Gitman MR, McTaggart L, Spinato J, Poopalarajah R, Lister E, Husain S, Kus JV. 2017. Antifungal susceptibility testing of Aspergillus spp. by using a composite correlation index (CCI)-based matrix-assisted laser desorption ionization-time of flight mass spectrometry method appears to not offer benefit over traditional broth microdilution testing. J Clin

show abstract

Characterization of intact microorganisms by MALDI mass spectrometry

Cited by 745 publications

References 100 publications

Identification of Highly Pathogenic Microorganisms by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Results of an Interlaboratory Ring Trial

Identification of Highly Pathogenic Microorganisms by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Results of an Interlaboratory Ring Trial

Intact spore MALDI-TOF mass spectrometry and proteomic analysis of Puccinia pathogenic fungi

Antifungal Susceptibility Testing of Aspergillus spp. by Using a Composite Correlation Index (CCI)-Based Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Method Appears To Not Offer Benefit over Traditional Broth Microdilution Testing

Contact Info

Product

Resources

About