Characterization of integrons and antimicrobial resistance genes in clinical isolates of Gram‐negative bacteria from Palestinian hospitals

Abstract

Sixty Gram-negative bacterial isolates were collected from Palestinian hospitals in 2006. Thirty-two (53.3%) isolates showed multidrug resistance phenotypes. PCR and DNA sequencing were used to characterize integrons and antimicrobial resistance genes. PCR screening showed that 19 (31.7%) and five (8.3%) isolates were positive for class 1 and class 2 integrons, respectively. DNA-sequencing results for the captured antimicrobial resistance gene cassettes within class 1 integrons identified the following genes: dihydrofolate reductases, dfrA1, dfrA5, dfrA7 , dfrA12, dfrA17 and dfrA25; aminoglycoside adenyltransferases, aadA1, aadA2, aadA5, aadA12 and aadB; aminoglycoside acetyltransferase, aac(6 )-Ib; and chloramphenicol resistance gene, cmlA1. ESBL were identified in 25 (41.7%) isolates. The identified ESBL were bla CTX-M-15 , bla CTX-M-56 , bla OXA-1 , bla SHV-1 , bla SHV-12 , bla SHV-32 and bla TEM-1 genes. Moreover, we characterized the plasmid-mediated quinolone resistance genes, aac(6 )-Ib-cr and qnrB2, which were detected in seven (11.7%) and two (3.3%) isolates, respectively. In this study various types of antibiotic resistance genes have been identified in Gram-negative bacteria from Palestinian hospitals, many of which are reported in the Middle East area for the first time.

Class 1 integron gene cassettes in multidrug-resistant Gram-negative bacteria in southern China

“…In the present study, we screened and characterised integrons in MDR Gram-negative bacterial isolates from Nanfang Hospital. A high frequency of integrons was observed among MDR Gram-negative bacteria (57.0%) [6,8], which corroborates well with a previous report [9]. A total of 45 different gene cassettes were observed in 825 isolates.…”

“…Their contribution to the prevalence of MDR Gram-negative bacteria has been demonstrated [6,7]. In the present study, we screened and characterised integrons in MDR Gram-negative bacterial isolates from Nanfang Hospital.…”

Clonal dissemination of a single Shigella sonnei strain among Iranian children during Fall 2012 in Tehran, I.R. Iran

“…Similar array of gene cassettes of class I integrons was primarily detected among enteric bacteria including Proteus, E. coli and Shigella spp. (Eftekhari et al, 2013;Hussein et al, 2009;Najibi et al, 2012). Moreover, comparable array of gene cassettes have been detected in Klebsiella pneumonia and UPEC (Lee et al, 2001).This may reveal the fact that the Enterobacteriaceae act as a reservoir of resistance genes that can be transferred to other species within or outside the family and alter the genetic contents and resistance potential in bacteria (Young and Amyes, 1986).…”

First Report of Extended-Spectrum β-Lactamases Among Clinical Isolates ofKlebsiella pneumoniaein Gaza Strip, Palestine

“…There is only one previous report about the detection of three clinical K. pneumoniae isolates in the West bank harboring non-ESBL SHVs, including SHV-1 and SHV-32, which are unable to confer resistance to broad-spectrum cephalosporins. 20 Nonetheless, numerous reports are available from Middle East and North African countries. 9,10 In this report, 14 of the16 K. pneumoniae clinical isolates harbored ESBLs of the CTX-M-1 group, including isolates encoding bla CTX-M-15 (n = 13) and bla CTX-M-3 (n = 1) genes.…”

“…There have been reports of bla CTX-M-15 , bla CTX-M-56 , bla OXA-1 , bla SHV-1 , bla SHV-12 , bla SHV-32 , and bla TEM-1 genes in Gram-negative bacteria isolated from Palestinian patients in West Bank. 20,21 Keeping in view the above facts, we have investigated the molecular characteristics of ESBL-producing K. pneumoniae isolates obtained from Al-Shifa Hospital, Balsam Hospital, and AL-Remal Martyrs Health Center in Gaza Strip, Palestine.…”