Class 1 integron gene cassettes in multidrug-resistant Gram-negative bacteria in southern China

K, Wu; Wang, Fengping; Sun, Jiufeng; Wang, Qian; Chen, Qing; Song, Yu; Rui, Yongyu

doi:10.1016/j.ijantimicag.2012.05.017

Cited by 52 publications

(36 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…IntI1 genes were presented in 76.7 % (23/30) of Enterobacteriaceae isolates, 66.7 % (32/48) of P. Of the 85 intI1 gene positive isolates, 78 showed individual amplicons that differed in size between *1 and *4 kb. No amplicon was obtained from the remaining seven isolates, which was probably due to the presence of atypical class 1 integrons or the larger variable region that could not be amplified [11,15]. Restriction fragments length polymorphism and sequencing analysis of these amplicons identified nineteen different resistant gene cassettes and fourteen different arrays.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Novel Class 1 Integrons in Multi-drug Resistant Isolates from Eastern China

Sun

Shao

et al. 2013

Indian J Microbiol

View full text Add to dashboard Cite

Integrons are mobile genetic elements able to capture, express and excise resistance genes, playing an important role in the spread of bacterial resistance. The present study was to investigate the occurrence and diversity of integrons in 120 clinical multi-drug resistant Gram-negative isolates from eastern China. Screening of integrons was performed by PCR and gene cassettes were further characterized by PCR-RFLP and sequencing. Class 1 integrons were detected in 70.8 % of isolates and no class 2 and class 3 integrons were detected in any isolates. A total of 19 resistant gene cassettes were identified, four representative of novel gene cassettes: an aacA3 variant (aacA3c), an aacA4 variant (aacA4 0 -17), a bla OXA variant (bla OXA-251 ), and a catB8 gene cassette interrupted by an insertion sequence IS10 (catB8::IS10). In addition, 14 cassette arrays were detected, including three novel integrons:gcuD1-aacA4 0 -17-gcu38B-catB8::IS10 (In712), aacA3c-aadA13-bla OXA-251 (In713) and dfrA1-gcu37-aadA5 (In714). The presence of novel integron structures in clinical isolates suggests hospital environments may favor the formation of novel combination of gene cassettes. Moreover, the high prevalence of integrons in multi-drug resistant isolates highlights the urgent need to employ effective means to avoid dissemination of drug-resistant bacteria.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Over the past decades, much work has been done on the epidemiology of integrons and their important roles in bacterial resistance in both clinical and natural environments [2,4,[8][9][10][11]. Surveillance of integrons therefore is essential for the investigation of MDR bacteria evolution.…”

Section: Introductionmentioning

confidence: 99%

Novel Class 1 Integrons in Multi-drug Resistant Isolates from Eastern China

Sun

Shao

et al. 2013

Indian J Microbiol

View full text Add to dashboard Cite

show abstract

“…To our knowledge, aacA4-catB8-aadA1 was not common among E. coli isolates [22,23]. However, this array was recently detected in clinical isolate of E. coli in Guangzhou, China [22]. The presence of this gene cassette in E. coli from cooked meat products in our study indicates that class 1 integrons may play an important role in the horizontal dissemination of antimicrobial resistance in the different bacterial species and the same species from different isolation sources.…”

Section: Discussionmentioning

confidence: 55%

“…The cassette array aacA4-catB8-aadA1, encoding tobramycin, chloramphenicol, and streptomycin resistance, respectively, has been found frequently in Acinetobacter isolates in many previous studies [19,20,21]. To our knowledge, aacA4-catB8-aadA1 was not common among E. coli isolates [22,23]. However, this array was recently detected in clinical isolate of E. coli in Guangzhou, China [22].…”

Section: Discussionmentioning

confidence: 75%

Characterization and horizontal transfer of class 1 integrons in Escherichia coli isolates from cooked meat products

Zhang

Jiang

et al. 2016

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Escherichia coli is a commensal bacterium in humans, animals, and the environment that is one of the microorganisms commonly resistant to antimicrobials. Cooked meat products, which are popular in China, are easily contaminated by E. coli during processing and storage. Methodology: In this study, a total of 75 E. coli isolates from cooked meat products in Henan province, China, were assayed for the presence of and horizontal transfer of class 1 integrons. Results: Class 1 integrons were detected in 11 (14.7%) of these isolates, and contained four groups of resistance gene cassettes, including dfrA17-aadA5, dfrA1-aadA1, dfrA12-orfF-aadA2, and an uncommon array of aacA4-catB8-aadA1. The transfer frequency of selected integron-positve donors ranged from 10 -6 to 10 -4 transconjugants per recipient cell, and the integron-containing DNA from the donors could be transferred to E. coli J53Az r with the transformation frequency of 10 -7 to 10 -5 . Conclusions: Class 1 integrons could be transferred to recipient E. coli J53 by conjugation and natural transformation. These findings suggest the role of commensal E. coli isolates from cooked meats as an important reservoir for integrons and the possible transfer of antimicrobial resistance genes to humans via the food chain.

show abstract

“…In the last decade, infections caused by MDR K. pneumoniae have increased intensely with corresponding increase in morbidity and mortality (Wu et al, 2012). In the contemporary study, MDR K. pneumoniae isolates encompassed 49.2% of the total K. pneumoniae isolates.…”

Section: Characteristics Of the Mdr K Pneumoniae Isolatesmentioning

confidence: 99%