Characterization of intracellular metabolites of axenic amastigotes of Leishmania donovani by 1H NMR spectroscopy

Gupta, Nidhi; Goyal, Neena; Singha, U. K.; Bhakuni, Vinod; Roy, Raja; Rastogi, Anil K.

doi:10.1016/s0001-706x(99)00020-0

Cited by 18 publications

(12 citation statements)

References 20 publications

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“…Another study reported detection of intracellular metabolites of L. donovani by 1 H NMR spectroscopy, and that Leishmania parasites contained hydroxybutyrate, acetate, lactate, alanine, and succinate, which are also present in urine. 35 It is likely that these metabolites are also present in human urine and are important for successful metabolism of parasites.…”

Section: Discussionmentioning

confidence: 99%

Effect of Human Urine on Cell Cycle and Infectivity of Leismania Species Promastigotes In Vitro

Allahverdiyev

Bağırova²,

Elcicek³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Abstract. In vitro cultivation of Leishmania parasites plays an important role in diagnosis and treatment of leishmaniasis and in vaccine and drug development studies. Conversely, long-term cultivation of Leishmania parasites usually results in decreased infectivity potential. Some studies reported a stimulatory effect of human urine in Leishmania promastigotes. However, there is no information about the effects of urine within culture on the infectivity of Leishmania parasites. Analysis of the effect of urine have showed that proliferation indexes were significantly increased in culture medium supplemented with human urine ( L. tropica = 38.17 ± 5.12, L. donovani = 34.74 ± 5.6, L. major = 34.22 ± 4.66, and L. infantum 35.88 ± 6.40) than in controls. Infection indexes were 13 ± 1.7 for L. tropica , 55 ± 2.2 for L. infantum , 41 ± 3.14 for L. donovani , and 49 ± 3.26 for L. major . Our results showed that human urine increased the infectivity and proliferation of Leishmania parasites.* Address correspondence to Adil M. Allahverdiyev,

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Section: Discussionmentioning

confidence: 99%

Effect of Human Urine on Cell Cycle and Infectivity of Leismania Species Promastigotes In Vitro

Allahverdiyev

Bağırova²,

Elcicek³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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“…In addition to identifying and quantifying amino acids, nucleotides, carboxylates and other molecules, they found that perchloric acid was the optimal extraction reagent [11]. 1 H-NMR metabolomics has been applied to other parasites to study, for example, the metabolites produced and released by Cryptococcus neoformans in culture media [37], the lipidome of Leishmania donovani [38], comparing intracellular metabolites produced by long-term in vitro cultured L. donovani axenic amastigotes with those produced by promastigotes and intracellular amastigotes [39], and the in vivo metabolism of Trypanosoma cruzi [40]. …”

Section: Approaches To Metabolomic Analysismentioning

confidence: 99%

Metabolomics and malaria biology

Lakshmanan

Rhee

Daily

2011

Molecular and Biochemical Parasitology

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Metabolomics has ushered in a novel and multi-disciplinary realm in biological research. It has provided researchers with a platform to combine powerful biochemical, statistical, computational, and bioinformatics techniques to delve into the mysteries of biology and disease. The application of metabolomics to study malaria parasites represents a major advance in our approach towards gaining a more comprehensive perspective on parasite biology and disease etiology. This review attempts to highlight some of the important aspects of the field of metabolomics, and its ongoing and potential future applications to malaria research.

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“…With increasing magnet strength and improved signal filtering the use of 1 H-NMR for metabolite mapping has steadily grown and yielded several studies mapping the metabolome of kinetoplastid parasites including L. donovani (Gupta et al, 1999) and T. cruzi (Penin et al, 1998), as well as the lipidome of L. donovani (Adosraku et al, 1993). A recent metabolic analysis of P. falciparum trophozoite extracts compared several extraction conditions and identified over 50 metabolites with measured concentrations in the high nM/low μM range for 40 separate metabolites (Teng et al, 2009).…”

Section: Laying the Groundwork: Profiling Parasite Metabolomesmentioning

confidence: 99%

Eating at the Table of Another: Metabolomics of Host-Parasite Interactions

Kafsack

Llinás

2010

Cell Host & Microbe

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The application of metabolomics, the global analysis of metabolite levels, to the study of protozoan parasites has become an important tool for understanding the host/parasite relationship and holds promise for the development of direly needed therapeutics and improved diagnostics. Research advances over the past decade have opened the door for a systems biology approach to protozoan parasites with metabolomics providing a crucial readout of metabolic activity. In this review we highlight recent metabolomic approaches to protozoan parasites, including metabolite profiling, integration with genomics, transcription, and proteomic analysis, as well as the use of metabolic fingerprints for the diagnosis of parasitic infections.

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Characterization of intracellular metabolites of axenic amastigotes of Leishmania donovani by 1H NMR spectroscopy

Cited by 18 publications

References 20 publications

Effect of Human Urine on Cell Cycle and Infectivity of Leismania Species Promastigotes In Vitro

Effect of Human Urine on Cell Cycle and Infectivity of Leismania Species Promastigotes In Vitro

Metabolomics and malaria biology

Eating at the Table of Another: Metabolomics of Host-Parasite Interactions

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