Characterization of Isoflavones Accumulation in Developing Leaves of Soybean (Glycine max) Cultivars

Lee, Jin Hwan; Ha, Tae Joung; Ko, Jong-Min; Cho, Kye Man; Im, Moo-Hyeog; Choung, Myoung-Gun

doi:10.3839/jksabc.2009.026

Cited by 11 publications

(4 citation statements)

References 15 publications

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“…The results of Lee et al [48], showed that the total contents of daidzein, glycitein, and genistein in soybean leaves have positively correlated with the growth period. In our study, the highest content of isoflavones was observed at vegetative stages (Table 3).…”

Section: Discussionmentioning

confidence: 95%

Phenolic Composition and Antioxidant Activities of Soybean (Glycine max (L.) Merr.) Plant during Growth Cycle

et al. 2019

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It is important to identify the growth stage at which the plant has the maximum antioxidant properties for the production of bioactive compounds from crops or agricultural by-products or for forage as a possible source of antioxidants in livestock. Therefore, we investigated the phenolic composition and antioxidant capacity of the aerial part of soybean at seven stages classified as vegetative stages (V5 and V6) and reproductive stages (R1, R2, R3, R4, and R5). Aqueous-methanol extracts were evaluated for their total phenolic content (TPC), ferric-reducing antioxidant power (FRAP), Trolox equivalent antioxidant capacity (TEAC), antioxidant activity as determined by photochemiluminescence assay (PCL-ACL), Fe2+ chelating ability, and antiradical activity against DPPH•. The extracts with the highest TPC content were obtained at stages V6 and R5. The phenolic compounds profile, as determined by DAD-HPLC, was characterized by 19 compounds, that differed significantly by growth stage (p < 0.05). Antioxidant tests showed significant differences among stages (p < 0.05). The lowest TEAC value was found for the R2 stage and the highest values for the R3 and R1 stages. FRAP values ranged from 623 to 780 μmol Fe2+/g extract. PCL-ACL values ranged from 516 to 560 μmol Trolox eq./g extract; Fe2+ chelation ability ranged from 36.5 to 51.7%. The highest antiradical activity against DPPH• was found in the extract from the V5 stage, which had the lowest EC50 value. The extracts of soybean plant can be used in pharmacy for the production of nutraceuticals by virtue of their good antioxidant activity and content of flavonols and other bioactive constituents.

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Section: Discussionmentioning

confidence: 95%

Phenolic Composition and Antioxidant Activities of Soybean (Glycine max (L.) Merr.) Plant during Growth Cycle

et al. 2019

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“…Bona (Ganzera et al 2008 ), or high levels of isoflavones in Glycine max cv. Saeolkong (Lee et al 2009 ). Our results for the cultivar of Chinese magnolia vine correspond with those findings.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical and biotechnological studies on Schisandra chinensis cultivar Sadova No. 1—a high utility medicinal plant

Szopa

Klimek-Szczykutowicz

Kokotkiewicz

et al. 2018

Appl Microbiol Biotechnol

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In the presented work, raw materials (fruits and leaves) and in vitro biomass of a highly productive Schisandra chinensis Sadova No. 1 cultivar (SchS) were evaluated for the production of therapeutically useful schisandra lignans (SL). In vitro cultures of SchS were initiated, followed by extensive optimization studies focused on maximizing secondary metabolite production, with the aim of establishing a sustainable source of SL. Different cultivation systems (agar, agitated, bioreactor), experiment times (10, 20, 30, 40, 50 and 60 days) and plant growth regulators (6-benzyladenine—BA and 1-naphthaleneacetic acid—NAA, from 0 to 3 mg/l) in Murashige-Skoog (MS) medium were tested. Moreover, an elicitation procedure was applied to bioreactor-grown microshoots in order to increase SL production. Validated HPLC-DAD protocol enabled to detect fourteen SL in the extracts from in vitro and in vivo materials. The main compounds in the in vitro cultures were as follows: schisandrin (max. 176.3 mg/100 g DW), angeloylgomisin Q (max. 85.1 mg/100 g DW), gomisin A (max. 71.4 mg/100 g DW) and angeloylgomisin H (max. 67.0 mg/100 g DW). The highest total SL content (490.3 mg/100 g DW) was obtained in extracts from the biomass of agar cultures cultivated for 30 days on the MS medium variant containing 3 mg/l BA and 1 mg/l NAA. This amount was 1.32 times lower than in fruit extracts (646.0 mg/100 g DW) and 2.04 times higher than in leaf extracts (240.7 mg/100 g DW). The study demonstrated that SchS is a rich source of SL, thus proving its value for medical, cosmetic and food industry.Electronic supplementary materialThe online version of this article (10.1007/s00253-018-8981-x) contains supplementary material, which is available to authorized users.

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“…The content of: ampelopsin, apigenin, daidzein, glycitein, hesperetin, hesperidin, isorhamnetin, kaempferol, luteolin, naryngin, quercetin, rutin, taxifolin was determined. The selection of the avonoid spectrum for analysis was based on literature data [73][74][75][76][77] .…”

Section: High-performance Liquid Chromatography Of Avonoidsmentioning

confidence: 99%

Antixenosis in Glycine max (L.) Merr against Acyrthosiphon pisum (Harris)

Stec

Kordan

Sergiel

et al. 2021

Preprint

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To reveal the antixenosis potential against the pea aphid Acyrthosiphon pisum (Harris) (Hemiptera: Aphididae) we analyzed the pea aphid survival and probing behavior, and the quantitative and qualitative variation of flavonoids in the leaves of selected soybean Glycine max (L.) Merr (Fabaceae) cultivars ‘Aldana’, ‘Annushka’, ‘Augusta’, ‘Madlen’, ‘Mavka’, ‘Simona’, ‘Violetta’, and ‘Viorica’. Aphid survival was drastically impeded on all cultivars. The electronic monitoring of aphid probing using the Electrical Penetration Graph (EPG) technique revealed that on all soybean cultivars, A. pisum readily probed into leaf tissues but the probes were usually terminated before reaching vascular tissues, which demonstrates the activity of antixenosis mechanisms in peripheral tissues epidermis and/or mesophyll in soybean leaves. The potency of antixenosis factors differed among soybean cultivars, which was reflected in differences in aphid survival and frequency and duration of phloem sap ingestion. Seven flavonoids were found: apigenin, daidzein, genistein, glycitein, isorhamnetin, kaempferol, and rutin, which occurred in different amount and proportion in individual cultivars. The content of apigenin and genistein in all soybean cultivars studied probably made them relatively unacceptable to A. pisum. Kaempferol in ‘Aldana’ might be responsible for the observed strong antixenosis resistance of this cultivar to A. pisum.

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Characterization of Isoflavones Accumulation in Developing Leaves of Soybean (Glycine max) Cultivars

Cited by 11 publications

References 15 publications

Phenolic Composition and Antioxidant Activities of Soybean (Glycine max (L.) Merr.) Plant during Growth Cycle

Phenolic Composition and Antioxidant Activities of Soybean (Glycine max (L.) Merr.) Plant during Growth Cycle

Phytochemical and biotechnological studies on Schisandra chinensis cultivar Sadova No. 1—a high utility medicinal plant

Antixenosis in Glycine max (L.) Merr against Acyrthosiphon pisum (Harris)

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