Characterization of key genes involved in anthocyanins biosynthesis in Nelumbo nucifera through RNA-Seq

Deng, Jiao; Li, Juanjuan; Su, Mengyue; Chen, Lei; Yang, Pingfang

doi:10.1016/j.aquabot.2021.103428

Cited by 5 publications

(5 citation statements)

References 47 publications

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“…Reverse transcription was performed using a First Strand cDNA Synthesis Kit (TOYOBO, Beijing, China). The expression profiles of some structure genes in the flavonoid pathway were analyzed by qRT-PCR as described previously (Deng et al 2021 ), each sample identical with the sample for the proteome assay was performed in three biological replicates. The Tartary buckwheat actin gene ( FtPinG0005405200.01 ) (Li et al 2019b ) was used as the reference, and the relative expression level of selected genes was calculated via the 2 −ΔΔCt method.…”

Section: Methodsmentioning

confidence: 99%

“…(Qin et al 2010 ). Additionally, Tartary buckwheat contains high content of flavonoid metabolites in many tissues (Li et al 2019b ; Uddin et al 2013 ; Li et al 2022 ; Deng et al 2021 ), which possess diverse health-promoting effects, such as anti-oxidative, anti-cancer, anti-tumor, anti-diabetic, anti-inflammatory, cardioprotective, hepatoprotective, as well as cholesterol-lowering activities (Zhu 2016 ; Lv et al 2017 ; Ge and Wang 2020 ). Therefore, Tartary buckwheat tea and alcohol are deeply loved by consumers for health care functions.…”

Section: Introductionmentioning

confidence: 99%

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Comparative proteomic analyses of Tartary buckwheat (Fagopyrum tataricum) seeds at three stages of development

Deng

Zhao

Huang

et al. 2022

Funct Integr Genomics

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Tartary buckwheat is among the valuable crops, utilized as both food and Chinese herbal medicine. To uncover the accumulation dynamics of the main nutrients and their regulatory mechanism of Tartary buckwheat seeds, microscopic observations and nutrient analysis were conducted which suggested that starch, proteins as well as flavonoid gradually accumulated among seed development. Comparative proteomic analysis of rice Tartary buckwheat at three different developmental stages was performed. A total of 78 protein spots showed differential expression with 74 of them being successfully identified by MALDI-TOF/TOF MS. Among them, granule bound starch synthase (GBSS1) might be the critical enzyme that determines starch biosynthesis, while 11 S seed storage protein and vicilin seemed to be the main globulin and affect seed storage protein accumulation in Tartary buckwheat seeds. Two enzymes, flavanone 3-hydroxylase (F3H) and anthocyanidin reductase (ANR), involved in the flavonoid biosynthesis pathway were identified. Further analysis on the expression profiles of flavonoid biosynthetic genes revealed that F3H might be the key enzyme that promote flavonoid accumulation. This study provides insights into the mechanism of nutrition accumulation at the protein level in Tartary buckwheat seeds and may facilitate in the breeding and enhancement of Tartary buckwheat germplasm.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative proteomic analyses of Tartary buckwheat (Fagopyrum tataricum) seeds at three stages of development

Deng

Zhao

Huang

et al. 2022

Funct Integr Genomics

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“…The red color in Asian lotus is determined by the contents of anthocyanin [40,41], which is controlled by key enzyme encoding genes, and their regulating transcription factors (TFs) such as MYB, basic-Helix-Loop-Helix (bHLH), WD40 in its biosynthetic pathway. Among all the enzyme encoding genes in this pathway, NnANS and NnUFGT seem to be the decisive two genes [42,43]. Several TFs including 5 MYB, 2 bHLH, and one WD-repeat genes, may be involved in the regulation of anthocyanin biosynthesis in lotus based on a transcriptome analysis [43].…”

Section: Identification Of Genes With Potential Application In Lotus ...mentioning

confidence: 99%

“…Among all the enzyme encoding genes in this pathway, NnANS and NnUFGT seem to be the decisive two genes [42,43]. Several TFs including 5 MYB, 2 bHLH, and one WD-repeat genes, may be involved in the regulation of anthocyanin biosynthesis in lotus based on a transcriptome analysis [43]. Among them, a bHLH gene NnTT8 was verified to regulate anthocyanin biosynthesis [44], whereas the yellow color of American lotus is determined by carotenoid, and no anthocyanin was detected [25,45].…”

Section: Identification Of Genes With Potential Application In Lotus ...mentioning

confidence: 99%

Studies on Lotus Genomics and the Contribution to Its Breeding

Deng

et al. 2022

IJMS

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Lotus (Nelumbo nucifera), under the Nelumbonaceae family, is one of the relict plants possessing important scientific research and economic values. Because of this, much attention has been paid to this species on both its biology and breeding among the scientific community. In the last decade, the genome of lotus has been sequenced, and several high-quality genome assemblies are available, which have significantly facilitated functional genomics studies in lotus. Meanwhile, re-sequencing of the natural and genetic populations along with different levels of omics studies have not only helped to classify the germplasm resources but also to identify the domestication of selected regions and genes controlling different horticultural traits. This review summarizes the latest progress of all these studies on lotus and discusses their potential application in lotus breeding.

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“…Certain MYB genes encode the key factors that have activating or suppressive effects on the transcript expression of the genes that encode the biosynthetic enzyme in both pathways (Chiu et al., 2010; Quattrocchio et al., 1999; Sagawa et al., 2016). The structural genes and MYB s related to anthocyanin biosynthesis have been identified in red‐petaled lotus (Deng et al., 2021; Sun et al., 2016), but candidate genes responsible for the differentiation of flower color between the two species remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Comparative analyses of American and Asian lotus genomes reveal insights into petal color, carpel thermogenesis and domestication

et al. 2022

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SUMMARY Nelumbo lutea (American lotus), which differs from Nelumbo nucifera (Asian lotus) morphologically, is one of the two remaining species in the basal eudicot family Nelumbonaceae. Here, we assembled the 843‐Mb genome of American lotus into eight pseudochromosomes containing 31 382 protein‐coding genes. Comparative analyses revealed conserved synteny without large chromosomal rearrangements between the genomes of American and Asian lotus and identified 29 533 structural variants (SVs). Carotenoid and anthocyanin pigments determine the yellow and red petal colors of American and Asian lotus, respectively. The structural genes encoding enzymes of the carotenoid and anthocyanin biosynthesis pathways were conserved between two species but differed in expression. We detected SVs caused by repetitive sequence expansion or contraction among the anthocyanin biosynthesis regulatory MYB genes. Further transient overexpression of candidate NnMYB5 induced anthocyanin accumulation in lotus petals. Alternative oxidase (AOX), uncoupling proteins (UCPs), and sugar metabolism and transportation contributed to carpel thermogenesis. Carpels produce heat with sugars transported from leaves as the main substrates, because there was weak tonoplast sugar transporter (TST) activity, and with SWEETs were highly expressed during thermogenesis. Cell proliferation‐related activities were particularly enhanced in the warmer carpels compared with stamens during the cold night before blooming, which suggested that thermogenesis plays an important role in flower protogyny. Population genomic analyses revealed deep divergence between American and Asian lotus, and independent domestication affecting seed, rhizome, and flower traits. Our findings provide a high‐quality reference genome of American lotus for exploring the genetic divergence and variation between two species and revealed possible genomic bases for petal color, carpel thermogenesis and domestication in lotus.

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Characterization of key genes involved in anthocyanins biosynthesis in Nelumbo nucifera through RNA-Seq

Cited by 5 publications

References 47 publications

Comparative proteomic analyses of Tartary buckwheat (Fagopyrum tataricum) seeds at three stages of development

Comparative proteomic analyses of Tartary buckwheat (Fagopyrum tataricum) seeds at three stages of development

Studies on Lotus Genomics and the Contribution to Its Breeding

Comparative analyses of American and Asian lotus genomes reveal insights into petal color, carpel thermogenesis and domestication

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