Characterization of Lactobacillus fermentum UCO-979C, a probiotic strain with a potent anti-Helicobacter pylori activity

García, Antonio Manuel Rodríguez; Navarro, Karen; Sanhueza, Enrique; Pineda, Susana; Pastene, Edgar; Quezada, M.; Henríquez, Karem; Karlyshev, Andrey V.; Villena, Julio; González, Carlos

doi:10.1016/j.ejbt.2016.11.008

Cited by 51 publications

(35 citation statements)

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“…L. fermentum UCO-979C [16] was obtained from the Bacterial Pathogenicity Laboratory culture collection at University of Concepción (Concepción, Chile). L. fermentum CRL973 was obtained from the CERELA-CONICET culture collection (Tucumán, Argentina).…”

Section: Microorganismsmentioning

confidence: 99%

“…L. fermentum CRL973 was obtained from the CERELA-CONICET culture collection (Tucumán, Argentina). Lactobacilli strains were grown in Mann-Rogosa-Sharpe Agar (MRS) (Difco, Lawrence, KS, USA) at 37 • C. After an overnight growth, bacteria were transferred to MRS broth and cultured at 37 • C until stationary phase [15][16][17]. Afterwards, the bacteria were pelleted (3000× g for 10 min), washed twice with phosphate-buffered saline (PBS) and suspended in specific media for in vivo or in vitro assays.…”

Section: Microorganismsmentioning

confidence: 99%

“…In this regard, our previous studies demonstrated the benefits of Lactobacillus fermentum UCO-979C against the pathogenic bacteria H. pylori, which were related to its capability of forming biofilms [15]. L. fermentum UCO-979C inhibited bacterial growth, reduced H. pylori urease activity, decreased pathogen adhesion to human gastric epithelial cells and beneficially regulated the inflammatory response in in vitro model by reducing the inflammatory chemokine IL-8 [16]. Besides, L. fermentum UCO-979C was able to differentially modulate the production of TNF-α, IFN-γ and IL-10 by gastric epithelial cells and macrophages, which may offer advantages in the protection against H. pylori infection by improving the clearance of the pathogen and the protection against inflammatory damage [17].…”

Section: Introductionmentioning

confidence: 96%

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The Exopolysaccharide of Lactobacillus fermentum UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against Helicobacter pylori Infection

et al. 2020

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Lactobacillus fermentum UCO-979C (Lf979C) beneficially modulates the cytokine response of gastric epithelial cells and macrophages after Helicobacter pylori infection in vitro. Nevertheless, no in vivo studies were performed with this strain to confirm its beneficial immunomodulatory effects. This work evaluated whether Lf979C improves protection against H. pylori infection in mice by modulating the innate immune response. In addition, we evaluated whether its exopolysaccharide (EPS) was involved in its beneficial effects. Lf979C significantly reduced TNF-α, IL-8, and MCP-1 and augmented IFN-γ and IL-10 in the gastric mucosa of H. pylori-infected mice. The differential cytokine profile induced by Lf979C in H. pylori-infected mice correlated with an improved reduction in the pathogen gastric colonization and protection against inflammatory damage. The purified EPS of Lf979C reduced IL-8 and enhanced IL-10 levels in the gastric mucosa of infected mice, while no effect was observed for IFN-γ. This work demonstrates for the first time the in vivo ability of Lf979C to increase resistance against H. pylori infection by modulating the gastric innate immune response. In addition, we advanced knowledge of the mechanisms involved in the beneficial effects of Lf979C by demonstrating that its EPS is partially responsible for its immunomodulatory effect.

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Section: Microorganismsmentioning

confidence: 99%

Section: Microorganismsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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The Exopolysaccharide of Lactobacillus fermentum UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against Helicobacter pylori Infection

et al. 2020

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“…Additionally, L. fermentum UCO-979C which has obtained from the human gut can inhibit the function of h. pylori by regulating the immune system. 7 them better for clinical purpose. [8][9][10][11] As we can see in the recent studies, microbiota like L. plantarum ZDY 2013 and L. fermentum UCO-979C have a great impact on the constraint of production of proinflammatory cytokines by h. pylori infection.…”

Section: Uco-979c In Order To Eradicate Bacterial Infection Such As Hmentioning

confidence: 99%

The Effect of Probiotics on Immune System

Ganjbakhsh¹,

Rezaee²

2017

JBMOA

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ConclusionRecent evidence reveals the impact of probiotics on the regulation of host homeostasis, involving immune health in humans. Also, the use of probiotics can protect the host against infection, reduce harmful bacteria, and stimulate immune response. However, more researches should be done to show the accurate probiotic mechanism to apply Abstract In recent years, the research into probiotics indicates that supplementing with probiotics is probably more important than taking a multi-vitamin or antibiotics. This paper presents the interaction between host-derived probiotics and immunomodulatory. Studies have revealed probiotics can produce a unique substance that affects the expression of particular genes which are related to immune regulation in humans. The effect of gastric microbiota such as Lactobacillus fermentum and Lactobacillus plantarum is an amazing example to demonstrate how they are able to regulate the immune system. In fact, the studies indicate that L. plantarum ZDY 2013 pretreatment can prevent gastric mucosal inflammation induced by h. pylori. In addition, L. fermentum UCO-979C-obtained from human gut shows an efficient growth in the rodent (Mongolian gerbil). It can stop stimulation to produce cytokines like IL8 by h. pylori in gastric adenocarcinoma human cells (AGS) cells.Citation: Ganjbakhsh SE, Rezaee P. The effect of probiotics on immune system.

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“…It shows the beneficial effect of probiotics on the immunomodulatory system. Additionally, L. fermentum UCO-979C which has obtained from the human gut can inhibit the function of H. pylori by regulating the immune system [7].…”

Section: The Role Of L Plantarum Zdy 2013 and L Fermentum Uco-979c mentioning

confidence: 99%

Untitled

2017

JBMOA

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In recent years, the research into probiotics indicates that supplementing with probiotics is probably more important than taking a multi-vitamin or antibiotics. This paper presents the interaction between host-derived probiotics and immunomodulatory. Studies have revealed probiotics can produce a unique substance that affects the expression of particular genes which are related to immune regulation in humans. The effect of gastric microbiota such as Lactobacillus fermentum and Lactobacillus plantarum is an amazing example to demonstrate how they are able to regulate the immune system. In fact, the studies indicate that L. plantarum ZDY 2013 pretreatment can prevent gastric mucosal inflammation induced by H. pylori. In addition, L. fermentum UCO-979C-obtained from human gut shows an efficient growth in the rodent (Mongolian gerbil). It can stop stimulation to produce cytokines like IL8 by H. pylori in gastric adenocarcinoma human cells (AGS)cells.

show abstract

Characterization of Lactobacillus fermentum UCO-979C, a probiotic strain with a potent anti-Helicobacter pylori activity

Cited by 51 publications

References 50 publications

The Exopolysaccharide of Lactobacillus fermentum UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against Helicobacter pylori Infection

The Exopolysaccharide of Lactobacillus fermentum UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against Helicobacter pylori Infection

The Effect of Probiotics on Immune System

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