Characterization of Lactobacillus from Algerian goat's milk based on phenotypic, 16S rDNA sequencing and their technological properties

Abstract

Nineteen strains of Lactobacillus isolated from goat’s milk from farms in north-west of Algeria were characterized. Isolates were identified by phenotypic, physiological and genotypic methods and some of their important technological properties were studied. Phenotypic characterization was carried out by studying physiological, morphological characteristics and carbohydrate fermentation patterns using API 50 CHL system. Isolates were also characterized by partial 16S rDNA sequencing. Results obtained with phenotypic methods were correlated with the genotypic characterization and 13 isolates were identified as L. plantarum, two isolates as L. rhamnosus and one isolate as L. fermentum. Three isolates identified as L. plantarum by phenotypic characterization were found to be L. pentosus by the genotypic method. A large diversity in technological properties (acid production in skim milk, exopolysaccharide production, aminopeptidase activity, antibacterial activity and antibiotic susceptibility) was observed. Based on these results, two strains of L. plantarum (LbMS16 and LbMS21) and one strain of L. rhamnosus (LbMF25) have been tentatively selected for use as starter cultures in the manufacture of artisanal fermented dairy products in Algeria.

Isolation, Identification and Screening of Potential Probiotic Bacteria in Milk from South African Saanen Goats

“…The 17 isolates were subjected to 16S rDNA sequencing for species level identification based on the 16S rDNA amplified by 27F and 1525R as reported by Marroki et al (2011). A single discrete PCR amplicon band of approximately 1.5 kilobase (kb) was observed and purified to remove contaminants.…”

In vitro Assessment of Antibiotic Resistance Pattern among Lactobacillus Strains Isolated from Goat Milk

“…Intrinsic resistance towards vancomycin might be due to the presence of D-Ala-D-lactate in the cell wall peptidoglycan, rather than the D-ala-Dala dipeptide moiety. Vancomycin resistance is chromosomally encoded and nontransmissible (Marroki et al, 2011).…”

Purification and Molecular Characterization of Two-Antimicrobial Peptides Produced by Lactobacillus plantarum DU10

“…J. Biol. Chem., 9 (2): 46-58, 2015 The obtained sequence was BLAST-analyzed using NCBI website and the results submitted to GenBank (Marroki et al, 2011;Kumar et al, 2010;Talpur et al, 2012;Amina et al, 2014).…”