Characterization of lectin isolated from Momordica charantia seed as a B cell activator

Huang, Li; Adachi, Takumi; Shimizu, Yusei; Goto, Yoshitaka; Toyama, Jun; Tanaka, Hidenori; Akashi, Ryo; Sawaguchi, Akira; Iwata, Hiroyuki; Haga, Takeshi

doi:10.1016/j.imlet.2008.10.004

Cited by 14 publications

(9 citation statements)

References 25 publications

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“…Momordicin activates and promotes B cell proliferation by inducing surface membrane immunoglobulin activity, while increasing B cell subsets CD86 (cell activation target point) expression, which plays a major role in humoral immunity. In addition, it can induce spleen cells to secrete large amounts of non-specific immunoglobulin IgM after 96 h co-culture and play a role in immune regulation [ 176 ]. In vitro, saponins isolated from M. charantia may promote IL-2 secretion by varying the ratio of T cells, enhancing phagocytic activity and improving immune function in aging mice [ 177 ].…”

Section: Biological Activitiesmentioning

confidence: 99%

Recent Advances in Momordica charantia: Functional Components and Biological Activities

Jia

Shen

Zhang

et al. 2017

IJMS

298

173

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Momordica charantia L. (M. charantia), a member of the Cucurbitaceae family, is widely distributed in tropical and subtropical regions of the world. It has been used in folk medicine for the treatment of diabetes mellitus, and its fruit has been used as a vegetable for thousands of years. Phytochemicals including proteins, polysaccharides, flavonoids, triterpenes, saponins, ascorbic acid and steroids have been found in this plant. Various biological activities of M. charantia have been reported, such as antihyperglycemic, antibacterial, antiviral, antitumor, immunomodulation, antioxidant, antidiabetic, anthelmintic, antimutagenic, antiulcer, antilipolytic, antifertility, hepatoprotective, anticancer and anti-inflammatory activities. However, both in vitro and in vivo studies have also demonstrated that M. charantia may also exert toxic or adverse effects under different conditions. This review addresses the chemical constituents of M. charantia and discusses their pharmacological activities as well as their adverse effects, aimed at providing a comprehensive overview of the phytochemistry and biological activities of M. charantia.

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Section: Biological Activitiesmentioning

confidence: 99%

Recent Advances in Momordica charantia: Functional Components and Biological Activities

Jia

Shen

Zhang

et al. 2017

IJMS

298

173

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“…Lectins can be found in various types of plants [1]–[3]. Plant lectins exhibit a variety of biological activities like anti-bacterial [4], anti-viral [5], anti-fungal [2] and anti-insect [3] activities.…”

Section: Introductionmentioning

confidence: 99%

“…The anti-pathogenic activities of plant lectins enable themselves to act as defense proteins to protect the plants from invasion of harmful organisms. Other than for the plants’ own uses, the plant lectins also exhibit other biological activities, such as inducing mitogenic response in mammalian splenocytes [1], [2], [6] and anti-proliferative effects toward tumor cells [7], [8]. These activities provide insights for plant lectins to be applied to humans for therapeutic uses, such as development of immunomodulatory drugs or anti-tumor drugs.…”

Section: Introductionmentioning

confidence: 99%

Isolation of a Glucosamine Binding Leguminous Lectin with Mitogenic Activity towards Splenocytes and Anti-Proliferative Activity towards Tumor Cells

et al. 2012

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A dimeric 64-kDa glucosamine-specific lectin was purified from seeds of Phaseolus vulgaris cv. “brown kidney bean.” The simple 2-step purification protocol involved affinity chromatography on Affi-gel blue gel and gel filtration by FPLC on Superdex 75. The lectin was absorbed on Affi-gel blue gel and desorbed using 1M NaCl in the starting buffer. Gel filtration on Superdex 75 yielded a major absorbance peak that gave a single 32-kDa band in SDS-PAGE. Hemagglutinating activity was completely preserved when the ambient temperature was in the range of 20°C–60°C. However, drastic reduction of the activity occurred at temperatures above 65°C. Full hemagglutinating activity of the lectin was observed at an ambient pH of 3 to 12. About 50% activity remained at pH 0–2, and only residual activity was observed at pH 13–14. Hemagglutinating activity of the lectin was inhibited by glucosamine. The brown kidney bean lectin elicited maximum mitogenic activity toward murine splenocytes at 2.5 µM. The mitogenic activity was nearly completely eliminated in the presence of 250 mM glucosamine. The lectin also increased mRNA expression of the cytokines IL-2, TNF-α and IFN-γ. The lectin exhibited antiproliferative activity toward human breast cancer (MCF7) cells, hepatoma (HepG2) cells and nasopharyngeal carcinoma (CNE1 and CNE2) cells with IC50 of 5.12 µM, 32.85 µM, 3.12 µM and 40.12 µM respectively after treatment for 24 hours. Flow cytometry with Annexin V and propidum iodide staining indicated apoptosis of MCF7 cells. Hoechst 33342 staining also indicated formation of apoptotic bodies in MCF7 cells after exposure to brown kidney bean lectin. Western blotting revealed that the lectin-induced apoptosis involved ER stress and unfolded protein response.

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“…Moreover, IPAF was capable of activating the development and maturation of purified B lymphocytes in view of the increased expression of CD69 and MHC class II, and the significant enhancement of IgM production. This characteristic selective activation of B lymphocytes by IPAF resembled that of the lectin isolated from Momordica charantia , which also specifically activated murine B lymphocytes, possibly through selective binding of cell specific carbohydrate [29]. Another similar mitogenic substance, which primarily activated B cells, inducing the cell proliferation and IgM secretion was found in the seeds of Ulex europeus [30].…”

Section: Discussionmentioning

confidence: 87%

“…For examples, lectins from mistletoe ( Viscum album ) were reported to be capable of elevating the population and activity of murine natural killer cell both in vitro and in vivo [24]; lectins from garlic ( Allium sativum ) were reported to proliferate murine thymocytes and splenocytes [26]; banana lectin from Musa pradisiac exhibited proliferation potential in CD3 + , CD4 + , and CD8 + cell populations of human PBMCs [27]; lectin from Narcissus tazetta displayed capability of activating murine macrophage and splenocytes [28]; lectin from the seeds of Momordica charantia activated murine B lymphocytes and induced their polyclonal IgM secretion in a T cell independent manner [29]. Since there hasn't been any report of Orchidaceae lectin regarding the immunomodulatory efficacy, this is the first article to elucidate the immunological function of an Orchidaceae lectin-like protein, IPAF, as an activator of murine B lymphocyte.…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning of a New Immunomodulatory Protein from Anoectochilus formosanus which Induces B Cell IgM Secretion through a T-Independent Mechanism

Kuan

Kuo

et al. 2011

PLoS ONE

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An immunomodulatory protein (IPAF) was purified and cloned from Anoectochilus formosanus, an Orchidaceae herbal plant in Asia. The major targeting immune cells of IPAF and its modulating effects toward B lymphocytes were investigated. Rapid amplification of cDNA ends (RACE) was conducted to clone the IPAF gene, and the obtained sequence was BLAST compared on the NCBI database. MACS-purified mouse T and B lymphocytes were stimulated with IPAF and the cell proliferation, activation, and Igs production were examined. IPAF comprised a 25 amino acids signal peptide and a 138 amino acids protein which was homologous to the lectins from Orchidaceae plant. IPAF selectively induced the cell proliferation in mouse splenic B lymphocytes but not T lymphocytes. The IPAF-induced B cells exhibited increased CD69 and MHC class II expression, and a dose- and time-dependent enhancement in IgM production. These results suggested potential benefits of IPAF to strengthen the humoral immunity.

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Characterization of lectin isolated from Momordica charantia seed as a B cell activator

Cited by 14 publications

References 25 publications

Recent Advances in Momordica charantia: Functional Components and Biological Activities

Recent Advances in Momordica charantia: Functional Components and Biological Activities

Isolation of a Glucosamine Binding Leguminous Lectin with Mitogenic Activity towards Splenocytes and Anti-Proliferative Activity towards Tumor Cells

Molecular Cloning of a New Immunomodulatory Protein from Anoectochilus formosanus which Induces B Cell IgM Secretion through a T-Independent Mechanism

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