Characterization of lymphocyte subpopulations in chronic lymphocytic leukemia

Rowlands, David T.; Daniele, Ronald P.; Nowell̀, Peter C.; Wurzel, Harold A.

doi:10.1002/1097-0142(197412)34:6<1962::aid-cncr2820340615>3.0.co;2-o

Cited by 38 publications

(1 citation statement)

References 29 publications

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“…However, other approaches (e.g. immunoglobulin gene rearrangement) have clearly indicated the clonal nature of CLL, and some of our early studies helped to demonstrate that this neoplasm was typically a B cell tumor and that the circulating T cells in CLL patients, although increased in numbers, were not part of the neoplasm (36). These clonal studies also contributed to the recognition that certain entities (e.g.…”

Section: Studies Of Neoplastic Lymphocytesmentioning

confidence: 99%

Studies of normal and neoplastic lymphocytes

Nowell̀

2002

Immunological Reviews

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This brief review encapsulates a nearly 50-year career in biomedical research, primarily studying human leukemias and lymphomas, but also involving normal lymphocytes. Early observations included the feasibility of bone marrow transplantation (and related problems with graft-vs.-host reactions); the mitogenic effect of phytohemagglutinin (and resultant human lymphocyte culture techniques); and early cytogenetic findings in human leukemias, both lymphocytic and myeloid (including the Philadelphia chromosome). Subsequent studies of normal human lymphocytes have contributed to our enormously expanding knowledge of their basic biology, especially regulatory pathways, both extracellular and intracellular. Further work with human lymphoid neoplasms has helped extend the early chromosomal findings to the specific genes involved, including several regulating apoptosis; and also contributed to the concept of clonal evolution as a basic underlying mechanism of tumorigenesis in general. This career has covered a period of remarkable growth of knowledge concerning both normal and neoplastic lymphocytes, with potential for many important future clinical applications; it has been a privilege to participate.

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Section: Studies Of Neoplastic Lymphocytesmentioning

confidence: 99%

Studies of normal and neoplastic lymphocytes

Nowell̀

2002

Immunological Reviews

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In vitrofunctional studies of mononuclear cells in patients with CLL. Evidence for functionally normal T lymphocytes and monocytes and abnormal B lymphocytes

Han

Dadey

1979

Cancer

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h a vitro functional studies of mononuclear cells from 34 patients with B-cell type CLL were investigated and the results of these studies were as follows: I) The T lymphocytes from patients with CLL were capable of responding normally to PHA or PWM, of inducing allogeneic normal B lymphocytes to respond to these mitogens and of stimulating normally to allogeneic lymphocytes in "one-way" mixed lymphocyte reaction; 2) The monocytes from these patients were capable of enhancing the T lymphocyte response to mitogens and of stimulating normally to allogeneic lymphocytes; and 3) The leukemic B lymphocytes were incapable of responding to mitogens even in the presence of normal T lymphocytes and their enhancer cell activity on T lymphocyte response or their stimulating capacity on allogeneic lymphocytes was depressed. These observations suggest that the T lymphocytes and monocytes from patients with CLL are functionally normal while the leukemic B lymphocytes from these patients are functionally abnormal.Cancer 43:109-117, 1979. HYTOMITOCENS SUCH AS phytohemag-P glutinin (PHA), concanavalin-A (Con A)and pokeweed mitogen (PWM) have been routinely used to stimulate human Iymphocytes to divide. The proliferate response of lymphocytes is usually assayed by the uptake of 3H-thymidine incorporation into DNA.There is no disagreement in the literature that fractionated human T lymphocytes from various sources are stimulated by these mitogens; on the other hand, there are many conflicting reports in regard to mitogen-induced proliferative response of fractionated human B servations suggest that the contradictory results of B lymphocyte proliferative response to phytomitogens are most likely explained by differences in the techniques used for isolation of B lymphocytes and, in some cases, to differences in the purity of the B lympho~y m p~o C y~e s~6

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T-cell chronic lymphocytic leukemia.Report of a case and review of the literature

Nair

Han

Minowada

1979

Cancer

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We recently observed a unique case of T-cell type chronic lymphocytic leukemia, documented by cell surface marker analyses. Immunologic tests at the time of diagnosis were as follows: 1) skin tests were negative; 2) in uitro lymphocyte responses to antigens or pokeweed mitogen were absent; 3) in uitro lymphocyte responses to phytohemagglutinin, concanavalin-A or allogeneic cells were low but significant; and 4) stimulating capacity of leukemic T cells were absent. Chromosomal analysis of bone marrow showed a pattern of 45 chromosomes with a marker chromosome. Terminal deoxynucleotidyl transferase activity was very low. Patient responded extremely well to COP (cyclophosphamide-oncovin-prednisone) therapy. Patient died of recurrent transitional cell carcinoma while his leukemia was in complete remission, approximately 20 months after the diagnosis of T-cell CLL.

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Characterization of lymphocyte subpopulations in chronic lymphocytic leukemia

Cited by 38 publications

References 29 publications

Studies of normal and neoplastic lymphocytes

Studies of normal and neoplastic lymphocytes

In vitrofunctional studies of mononuclear cells in patients with CLL. Evidence for functionally normal T lymphocytes and monocytes and abnormal B lymphocytes

T-cell chronic lymphocytic leukemia.Report of a case and review of the literature

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