Characterization of Lymphocytes in Canine Gastrointestinal Lymphoma

Coyle, Kellie A.; Steinberg, Howard

doi:10.1354/vp.41-2-141

Cited by 75 publications

(89 citation statements)

References 14 publications

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“…This is correlated with other recent studies suggesting that most digestive tract lymphomas in dogs come from T lymphocytes and often have epitheliotropism (Coyle and Steinberg, 2004;Ozaki et al, 2006).…”

Section: Resultssupporting

confidence: 91%

Immunohistochemical Characterization of Canine Lymphomas

Cora¹,

Gal²,

Taulescu³

et al. 2017

BUASVMCN-VM

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Lymphomas occur by clonal expansion of lymphoid cells and have distinctive morphological and immunophenotypic features. Determination of canine lymphoma immunophenotype is useful for accurate prognosis and further therapy. In the suggested study, we performed an immunohistochemical evaluation of some cases with canine lymphoma diagnosed in the Department of Pathology (Faculty of Veterinary Medicine, ClujNapoca, Romania), in order to characterize them. The investigation included 39 dogs diagnosed with different anatomical forms of lymphoma, following necropsy analysis or assessment of biopsies. The diagnosis of lymphoma was confirmed by necropsy and histopathology (Hematoxylin-eosin stain) examinations. The collected specimens were analyzed by immunohistochemistry technique (automatic method) using the following antibodies: CD3, CD20, CD21 and CD79a. The analyzed neoplasms were characterized as follows: about 64.10% of cases were diagnosed as B-cell lymphomas, 33.34% of cases as T-cell lymphomas, whereas 2.56% of cases were null cell type lymphomas (neither B nor T). Most of multicentric (80%), mediastinal (60%) and primary central nervous system lymphomas (100%) had B immunophenotype, while the majority of cutaneous (80%) and digestive (100%) lymphomas had T immunophenotype. Immunohistochemical description of canine lymphomas can deliver some major details concerning their behavior and malignancy. Additionally, vital prognosis and efficacy of some therapeutic protocols are relying on the immunohistochemical features of canine lymphoma.

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Section: Resultssupporting

confidence: 91%

Immunohistochemical Characterization of Canine Lymphomas

Cora¹,

Gal²,

Taulescu³

et al. 2017

BUASVMCN-VM

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“…38 Other studies identified between 63% and 75% of gastrointestinal lymphomas as having a T-cell phenotype, but these studies also examined the stomach, which is known to have a higher incidence of B-cell lymphomas in dogs and cats. 4,11,20,39,44 The only statistically significant morphologic parameter for differentiating IBD from lymphoma was the presence of intraepithelial infiltrations in both surface and crypt epithelium. In contrast to cats, a significant number of dogs with a diagnosis of IBD based on the combined HE, IHC, and PARR results exhibited marked intraepithelial infiltrates.…”

Section: Discussionmentioning

confidence: 99%

Distinguishing Intestinal Lymphoma From Inflammatory Bowel Disease in Canine Duodenal Endoscopic Biopsy Samples

et al. 2014

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Inflammatory bowel disease (IBD) and intestinal lymphoma are intestinal disorders in dogs, both causing similar chronic digestive signs, although with a different prognosis and different treatment requirements. Differentiation between these 2 conditions is based on histopathologic evaluation of intestinal biopsies. However, an accurate diagnosis is often difficult based on histology alone, especially when only endoscopic biopsies are available to differentiate IBD from enteropathy-associated T-cell lymphoma (EATL) type 2, a small cell lymphoma. The purpose of this study was to evaluate the utility of histopathology; immunohistochemistry (IHC) for CD3, CD20, and Ki-67; and polymerase chain reaction (PCR) for antigen receptor rearrangement (T-cell clonality) in the differential diagnosis of severe IBD vs intestinal lymphoma. Endoscopic biopsies from 32 dogs with severe IBD or intestinal lymphoma were evaluated. The original diagnosis was based on microscopic examination of hematoxylin and eosin (HE)-stained sections alone followed by a second evaluation using morphology in association with IHC for CD3 and CD20 and a third evaluation using PCR for clonality. Our results show that, in contrast to feline intestinal lymphomas, 6 of 8 canine small intestinal lymphomas were EATL type 1 (large cell) lymphomas. EATL type 2 was uncommon. Regardless, in dogs, intraepithelial lymphocytes were not an important diagnostic feature to differentiate IBD from EATL as confirmed by PCR. EATL type 1 had a significantly higher Ki-67 index than did EATL type 2 or IBD cases. Based on the results of this study, a stepwise diagnostic approach using histology as the first step, followed by immunophenotyping and determining the Ki67 index and finally PCR for clonality, improves the accuracy of distinguishing intestinal lymphoma from IBD in dogs.

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“…In contrast to humans (Sagaert et al 2012), most cases of primary GI lymphomas in dogs are of T-cell origin (Couto et al 1989;Coyle and Steinberg 2004). Carrasco et al (2015) reported a 100% prevalence of T-cell alimentary lymphomas, but the samples were taken from duodenum only.…”

Section: Discussionmentioning

confidence: 98%

“…The small intestine is most frequently involved, followed by the stomach (Coyle and Steinberg 2004).…”

mentioning

confidence: 99%

Gastrointestinal B-lymphoblastic lymphoma in a dog: a case report

Borska¹,

Husník²,

Fictum³

et al. 2018

Vet. Med. - Czech

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ABSTRACT:A four-year-old Bullmastiff weighing 44 kg was presented with a 14-day history of weight loss, vomiting and diarrhoea. Abdominal ultrasonography showed the presence of abdominal lymphadenopathy and thickening of the wall of the descending colon. Esophagogastroduodenoscopy and colonoscopy with biopsy were performed. Histological examination revealed a high-grade lymphoblastic lymphoma, flow cytometric analysis detected malignant cells of the immature B phenotype. PCR for antigen receptor rearrangement confirmed IgH monoclonality pointing together with immunophenotyping to B-cell lymphoma. The dog was treated using a multi-agent chemotherapy protocol. The overall survival time was 487 days. This was an unusual case of primary gastrointestinal B-lymphoblastic lymphoma in a dog with survival equivalent to that of the multicentric form.Keywords: canine; alimentary; lymphoid neoplasia; immunophenotyping; PARR; chemotherapy List of abbreviations:B-LBL = B-lymphoblastic lymphoma, FC = flow cytometry, FNAB = fine-needle aspiration biopsy, GI = gastrointestinal, LPE = lymphocytic-plasmacytic enteritis, PARR = polymerase chain reaction for antigen receptor rearrangement

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Characterization of Lymphocytes in Canine Gastrointestinal Lymphoma

Cited by 75 publications

References 14 publications

Immunohistochemical Characterization of Canine Lymphomas

Immunohistochemical Characterization of Canine Lymphomas

Distinguishing Intestinal Lymphoma From Inflammatory Bowel Disease in Canine Duodenal Endoscopic Biopsy Samples

Gastrointestinal B-lymphoblastic lymphoma in a dog: a case report

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