Characterization of Mannanase from a Novel Mannanase-Producing Bacterium

Abstract

Locust bean gum (LBG) was employed to screen mannanase-producing bacteria. The bacterium with highest mannanase ability was identified as Paenibacillus cookii. It revealed highest activity (6.67 U/mL) when cultivated in 0.1% LBG with 1.5% soytone and 0.5% tryptone after 4 days incubation at 27 °C. Its mannanase was purified to electrophoretical homogeneity after DEAE-Sepharose and Sephacryl S-100 separation. The purified mannanase, with an N-terminus of GLFGINAY, had pH and temperature optimum at 5.0 and 50 °C, respectively, and was stable at pH 5.0−7.0, ≤50 °C. It was strongly activated by β-mercaptoethanol, dithiothreitol, cysteine, and glutathione, but inhibited by Hg 2+ , Cu 2+ , Zn 2+ , Fe 3+ , PMSF, iodoacetic acid, and EDTA. According to substrate specificity study, the purified mannanase had high specificity to LBG and konjac.

Biochemical characterization and cloning of an endo-1,4-β-mannanase from Bacillus subtilis YH12 with unusually broad substrate profile

“…The utilization of mannanases along with other xylanases can equally facilitate lignin removal in pulp bleaching. On the other hand, it showed relatively high activity on guar, xanthan, and fenugreek gums, which was significantly different from the report by Yin et al [33]. The mannanase can degrade the polysaccharides including mannan, glucomannan, and galactomannan.…”

Selection ofBacillus subtilisUS191 as a mannanase‐producing probiotic candidate

“…1B). The same pH of optimal Bacillus β-mannanase activity has been reported by Yin et al [30] and Bo et al [35]. However, Pangsri et al [36] and Zhou et al [37] have characterized Bacillus-derived β-mannanases with optimal activity at pH 6 and 9.5, respectively.…”

“…The β-mannanase activities obtained using LBG as mannan source were higher than the values corresponding to mannanase production by many Bacillus strains (activities ranging from 0.001 to 0.8 U mL −1 ) as described by Adebayo-Tayo et al [18] and Abe et al [28]. However, higher enzyme titers were reported by Srivastava et al [29] and Yin et al [30] who showed that the extracellular β-mannanase activity produced by Bacillus sp. CFR1601 and Paenibacillus cooki, was of 12.62 and 6.67 U mL −1 , respectively.…”

“…and Yin et al. who showed that the extracellular β‐mannanase activity produced by Bacillus sp. CFR1601 and Paenibacillus cooki , was of 12.62 and 6.67 U mL −1 , respectively.…”

Effect of β‐mannanase domain from Trichoderma reesei on its biochemical characters and synergistic hydrolysis of sugarcane bagasse

“…β ‐mannanase has been identified and produced in many organisms, including bacteria, fungi, actinomycetes, plants and mollusks , . At present, research on β ‐mannanase has mainly focused on gene cloning, expression, characterization, production and structural analysis , .…”