Characterization of marine microalga, Scenedesmus sp. strain JPCC GA0024 toward biofuel production

Abstract

A marine microalga, strain JPCC GA0024 was selected as high amount of neutral lipid producers from marine microalgal culture collection toward biofuel production. The strain was tentatively identified as Scenedesmus rubescens by 18S rDNA analysis. The growth of strain JPCC GA0024 was influenced by artificial seawater concentrations. The optimum growth of 0.79 g/l was obtained at 100% artificial seawater. The lipid accumulation reached 73.0% of dry cell weight at 100% artificial seawater without additional nutrients for 11 days. Gas chromatography/mass spectrometry analysis indicates that lipid fraction mainly contained hydrocarbons including mainly hexadecane (C(16) H(34)) and 1-docosene (C(22) H(44)). Furthermore, calorimetric analysis revealed that the energy content of strain JPCC GA0024 was 6,160 kcal/kg (25.8 MJ/kg) of calorific value, which was equivalent to the coal engery. The strain JPCC GA0024, S. rubescens, will become a promising resource that can grow as a dominant species in the seawater for the production of both liquid and solid biofuels.

Screening and Evaluation of Some Green Algal Strains (Chlorophyceae) Isolated from Freshwater and Soda Lakes for Biofuel Production

“…Neutral lipids including hydrocarbons and triglycerides were stained in yellow, while polar lipids were stained in red [30]. In our observations, a large number of algal cells emitted yellow fluorescence indicating the presence of neutral lipids including hydrocarbons and triglycerides as shown in Figure 2 [31,32].…”

Cultivation of freshwater microalga <i>Scenedesmus</i> sp. using a low-cost inorganic fertilizer for enhanced biomass and lipid yield

“…In this context, the microalga Scenedesmus sp. IMMTCC-6 was found to be better in comparison with previously reported results of related strains (Hodaifa et al, 2009;Matsunaga et al, 2009;Morais and Costa, 2007).…”

Isolation and characterization of marine bacteria capable of utilizing phthalate

“…Marine bacteria were collected from the seawater sample by filtration using the membrane filters (diameter 47 mm, pore size 0.45 lm; Nihon Millipore, Tokyo), and then suspended in 30 ml of commercial artificial seawater medium Daigo's IMK-SP which is made by dissolving 252 mg of Daigo's IMK medium (Matsunaga et al 2009) in 1 l of Daigo's artificial seawater SP (Nihon Pharmaceutical, Osaka). A 1 ml suspension of each sample was inoculated into 4 ml of IMK-SP containing 5 mM sodium phthalate and the mixture was incubated at 25°C on a rotary shaker at 100 rev/min.…”