Characterization of membrane homing receptors in two cloned murine hemopoietic progenitor cell lines.

Matsuoka, Takashi; Hardy, Cheryl L.; Tavassoli, Mehdi

doi:10.1172/jci113975

Cited by 30 publications

(20 citation statements)

References 34 publications

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“…The data shown in table 1 demonstrate that the two cloned normal hemopoietic cells, FDCP-1 and B6SUT, bound neoglycoproteins, G-BSA and M-BSA, with a sim ilar pattern as described before [5], Both cells bound the l25I-ligand as a function of concentration without giving a saturation plateau (total binding). In the presence of excess unlabeled ligand, the binding of labeled ligand was linear and this was considered nonspecific binding.…”

Section: Binding O F Neoglycoproteins To Cloned Hemopoietic Cellssupporting

confidence: 59%

“…In this regard, they are similar to fully mature blood cells that lack homing receptors [5], The significance of this finding in the pathobiology of leuke mia is twofold. Firstly, it is possible that leukemic cells have an insufficient number of homing receptors, so that their binding to the stroma is not as efficient as it should be.…”

Section: Introductionmentioning

confidence: 74%

“…The ability of intravenously transplanted hemo poietic cells to selectively seek and seed hemopoietic tis sues is known as 'homing' [1][2][3][4], This ability is conferred upon hemopoietic progenitors by the presence of mem brane receptors known as homing receptors which are lost as differentiation occurs [5,6]. These receptors are C-type lectins [7] with galactosyl and mannosyl specific ities capable of binding and interacting with specific con figurations of membrane glycoconjugate on hemopoietic stromal cells [1,2,5,8,9], This interaction can lead to further cellular events which can perpetuate the pro cesses of proliferation and/or differentiation of progeni tor cells [10], By mediating the binding of progenitor cells to hemopoietic stroma, homing receptors also pro vide a gradient of these progenitor cells between blood and hemopoietic tissues so that while progenitor cells can move between various hemopoietic compartments, their concentration remains high in the hemopoietic organs, but low in the blood.…”

Section: Introductionmentioning

confidence: 99%

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Measurement of Homing Receptors on the Surface of Leukemic and Nonleukemic Cell Lines

Hardy¹,

Omoto²,

Tavassoli³

1990

Pathobiology

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Selective homing of hemopoietic progenitor cells to hemopoietic organs is the initial event in hemopoiesis. At a molecular level, it is mediated by a membrane receptor on the surface of hemopoietic progenitor cells. The receptor molecule then binds selectively to a glycoconjugate on the surface of bone marrow stromal cells. The molecular nature of this receptor has been shown previously to be a lectin with specificity for an as yet unknown configuration of galactosyl and mannosyl residues of the glycoconjugate. Normal murine progenitor cell lines, FDCP-1 and B6SUT, were found to possess this homing receptor on their cell membrane, and they also bind well to hemopoietic stroma. However, when three human leukemic cell lines, U937, HL60 and K562, were probed for the presence of this receptor, they were found to be deficient in the homing protein. Further, these cells showed little ability to bind the hemopoietic stromal cell line, GB1/6. These findings suggest that leukemic cells experience a loss, or at least alteration, of homing receptor molecule during leukemic transformation. This can result in a diminished ability to bind to marrow stroma. The lack of homing function may be of pathogenetic significance in the lack of differentiation of leukemic cells and may also contribute to the dissemination of leukemic cells in the circulation.

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Section: Binding O F Neoglycoproteins To Cloned Hemopoietic Cellssupporting

confidence: 59%

Section: Introductionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

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Measurement of Homing Receptors on the Surface of Leukemic and Nonleukemic Cell Lines

Hardy¹,

Omoto²,

Tavassoli³

1990

Pathobiology

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“…1,2 Tavassoli et al [3][4][5] described specific homing receptors and cytokine involvement 6 by which the homing process might be regulated. Papayannopoulou et al [7][8][9] showed that the VLA-4/VCAM-1 adhesion pathway plays a significant role in the homing process.…”

Section: Discussionmentioning

confidence: 99%

Hematopoietic progenitor cells from allogeneic bone marrow transplant donors circulate in the very early post-transplant period

Katayama

Mahmut

Takimoto

et al. 1999

Bone Marrow Transplant

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Summary:Despite the therapeutic efficacy of allogeneic bone marrow transplantation (allo-BMT), circulating hematopoietic progenitor cells after bone marrow transplantation have not been well characterized. In the present study, we focused on these 'post-transplant circulating progenitor cells (PTCPC)' which may be on their way to bone marrow. We analyzed the number of myeloid progenitor cells (CFU-GM) per 10 ml of peripheral blood (PB) on days 0 (just before transplantation), 1 (8-15 h after the completion of transplantation), 2, 3, 5, 7, 10, 14, 17, 21, 28 and 35 after allo-BMT in five transplant patients using a standard methylcellulose assay. In addition, high proliferative potential colony-forming cells (HPP-CFC) of the harvested donor bone marrow (BM) and day 1 PB of recipients were assayed in five patients. The origin of HPP-CFC from day 1 PB was analyzed by polymerase chain reaction of a DNA region containing a variable number of tandem repeats. The replating potential of these HPP-CFC was evaluated by a secondary colony assay. The proportion of CD38 negative cells among CD34 ؉ cells in the harvested BM and day 1 PB was evaluated by two-color flow cytometric analysis. The number of CFU-GM on day 1 ranged from 6 to 73/10 ml PB, and became undetectable on day 5. The reappearance of PTCPC was observed on day 14, along with hematopoietic recovery. The proportion of HPP-CFC among myeloid colonies from day 1 PB was significantly higher than that from harvested BM (44.3 ؎ 10.4% vs 11.3 ؎ 2.1%, respectively, n = 5, P = 0.0030). These HPP-CFC from day 1 PB were confirmed to be of donor origin. More than 90% of these HPP-CFC had replating potential. Two-color flow cytometric analysis revealed that the proportion of CD34 ؉ CD38 negative cells was significantly higher in day 1 PB than in the harvested BM (61.0 ؎ 16.5% vs 9.3 ؎ 3.5%, respectively, n = 7, P = 0.0002). These observations suggest that both primitive and committed transplanted myeloid progeniCorrespondence: Dr Y Katayama, Kobe West City Hospital, 2-4 Ichibancho, Nagata-ku, Kobe, Hyogo 653-0013, Japan Received 17 August 1998; accepted 19 October 1998 tor cells may circulate in the very early period following allo-BMT.

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“…[6][7][8] On the basis of these assumptions, we analyzed the expression of these specific endogenous sugar receptors on peripheral blood PMNL by means of a panel of labelled (neo)glycoproteins. The aim of the work was to investigate whether the level of expression of these receptors is influenced by prolonged exposure to anaesthetic agents and surgery.…”

Section: Nous Savons Que L'anesth#sie Et La Chirurgie D#priment La Fomentioning

confidence: 99%

Changes of expression of endogenous sugar receptors by polymorphonuclear leukocytes after prolonged anaesthesia and surgery

1992

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Anaesthesia and surgery are known to depress granulocyte function in the early postoperative period, leading to deterioration of the immune defence against infection. Carbohydratelectin interactions may play an important role in the activities of phagocytic cells in that theyGeneral anaesthesia and surgery are known to influence immune cell function. The consequent impairment of defence mechanisms leads to a higher risk of postoperative infection. The clinical manifestation is dependent on several factors, i.e., inherited conditions of the immune system, secondary immunodeficient states due to malnutrition, underlying malignant disease, immunosuppressive therapy as well as the degree of surgical trauma, the length of operation and the type of anaesthetic technique used.Peripheral blood polymorphonuclear leukocytes (PMNL) are involved in the first line defence against invading microorganisms. The depressive effect of nitrous CAN J ANAESTH 1992 / 39:2 / pp 143--50

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Characterization of membrane homing receptors in two cloned murine hemopoietic progenitor cell lines.

Cited by 30 publications

References 34 publications

Measurement of Homing Receptors on the Surface of Leukemic and Nonleukemic Cell Lines

Measurement of Homing Receptors on the Surface of Leukemic and Nonleukemic Cell Lines

Hematopoietic progenitor cells from allogeneic bone marrow transplant donors circulate in the very early post-transplant period

Changes of expression of endogenous sugar receptors by polymorphonuclear leukocytes after prolonged anaesthesia and surgery

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