Characterization of metabolic network of oxalic acid biosynthesis through RNA seq data analysis of developing spikes of finger millet ( Eleusine coracana ): Deciphering the role of key genes involved in oxalate formation in relation to grain calcium accumulation

Akbar, Naved; Gupta, S. C.; Tiwari, Apoorv; Singh, Kashmir; Kumar, Anil

doi:10.1016/j.gene.2018.01.071

Cited by 21 publications

(7 citation statements)

References 40 publications

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“…As mentioned in the introduction, oxalate is synthesized through three precursors, which are glycolic/glyoxylic, oxaloacetic, and AA 21,39 . Based on the findings of previous studies, glyoxylate in microbes, some plants, and animals; oxaloacetate in fungi, bacteria, and actinomycetes; and ascorbate in most plants are the primary substrate for oxalate synthesis 23,43‐45 . As shown in the biosynthetic models obtained in this paper, except for model c, the precursor of ascorbate does not play a significant role in oxalate production.…”

Section: Resultsmentioning

confidence: 60%

“…21,39 Based on the findings of previous studies, glyoxylate in microbes, some plants, and animals; oxaloacetate in fungi, bacteria, and actinomycetes; and ascorbate in most plants are the primary substrate for oxalate synthesis. 23,[43][44][45] As shown in the biosynthetic models obtained in this paper, except for model c, the precursor of ascorbate does not play a significant role in oxalate production. In this regard, the interpretation could probably be that the primary precursor of oxalate production in spinach populations, except for cluster C, is something other than AA.…”

Section: Path Diagram Analysismentioning

confidence: 59%

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High and low oxalate content in spinach: an investigation of accumulation patterns

et al. 2021

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BACKGROUND Oxalic acid is a common antinutrient in the human diet, found in large quantities in spinach. However, spinach is highly regarded by vegetable producers because of its nutritional content and economic value. One of the primary purposes of spinach‐breeding programs is to improve the nutritional value of spinach by adjusting oxalate accumulation. Knowledge of the biosynthetic patterns of oxalic acid, and its different forms, is important for a better understanding of this process. RESULTS We found three biosynthetic patterns of accumulation and concentration of oxalates. Two of them are related to the maximum type and one is related to the minimum type. We also developed a general model of variations in these compounds in the genotypes that were studied. CONCLUSION This study introduced a unique type of spinach with high oxalate accumulation, which could be particularly suitable for consumption. This had the highest ratio of insoluble oxalate to soluble oxalate. It also accumulated more ascorbic acid (AA) than other types. Our findings in this study also indicate a small role for AA as a precursor to oxalate production in spinach, possibly confirming the significant role of glyoxylate as the most critical precursor in this plant. © 2021 Society of Chemical Industry.

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Section: Resultsmentioning

confidence: 60%

Section: Path Diagram Analysismentioning

confidence: 59%

High and low oxalate content in spinach: an investigation of accumulation patterns

et al. 2021

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“…Overall, the production of millets is expected to increase in the coming years. For example, the overall production of barley, rice and corn were better and almost same in the year of 2016-17 but the production of madua/ ragi (finger millet), Jowar (Sorghum) and bajra (pearl millet) were lower [15] (Figure 1). On the other hand, the population of 1.343 billion, household cereal demand works out to 221 million tons However, an estimated in the production of sorghum will be 35.0 million tons, which will contribute alone 68% of total production of rabi.…”

Section: Production and Availabilitymentioning

confidence: 99%

Nutrients Anti-Nutrients Interaction in Millets and its Effect on Malnutrition

Akbar¹

2022

Act Scie Nutr

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Malnutrition problem, which is derived from having insufficient amount of nutrients like minerals, essential vitamins and other macronutrients (proteins and carbohydrates) in our daily diet. After the pandemic, the prevalence of under nourishment jumped from 8.0 in 2019 to around 9.3 percent in 2020 and continued to rise in 2021. Malnutrition problem can be reduced by adding the nutrients in our daily diet. Millets are the excellent source of minerals, vitamins, proteins and carbohydrates. But some anti-nutrients (oxalate, phytate) present on them interact with nutrients (like minerals, vitamins) and reduce its bioavailability in millets. To overcome this malnutrition problem, anti-nutrients should be removed in crops like millets. There are many biofortification methods used by scientists to improve the nutritional content time to time. However, it is necessary to know the current status of these antinutrients and identify those genes which are responsible for the accumulation of these anti-nutrients in millets. Thus, for better human health and physical wellbeing, it is most important to take bio-fortified foods containing fewer anti-nutrients.

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“…The study identified 12,893 unigenes specifically expressed in low moisture stress. Following this study, Akbar et al [4] had performed RNA-seq of developing spikes to dissect the oxalic acid biosynthesis pathway in finger millet. The data concluded that ascorbate-tartrate pathway could be a predominant player in oxalic acid biosynthesis, and this could be useful in studying the nutrients-antinutrients interactions in this crop.…”

Section: Transcriptomicsmentioning

confidence: 99%

Genetic and genomic resources, and breeding for accelerating improvement of small millets: current status and future interventions

Vetriventhan

Azevedo

Upadhyaya

et al. 2020

Nucleus

120

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Current agricultural and food systems encourage research and development on major crops, neglecting regionally important minor crops. Small millets include a group of small-seeded cereal crops of the grass family Poaceae. This includes finger millet, foxtail millet, proso millet, barnyard millet, kodo millet, little millet, teff, fonio, job's tears, guinea millet, and browntop millet. Small millets are an excellent choice to supplement major staple foods for crop and dietary diversity because of their diverse adaptation on marginal lands, less water requirement, lesser susceptibility to stresses, and nutritional superiority compared to major cereal staples. Growing interest among consumers about healthy diets together with climate-resilient features of small millets underline the necessity of directing more research and development towards these crops. Except for finger millet and foxtail millet, and to some extent proso millet and teff, other small millets have received minimal research attention in terms of development of genetic and genomic resources and breeding for yield enhancement. Considerable breeding efforts were made in finger millet and foxtail millet in India and China, respectively, proso millet in the United States of America, and teff in Ethiopia. So far, five genomes, namely foxtail millet, finger millet, proso millet, teff, and Japanese barnyard millet, have been sequenced, and genome of foxtail millet is the smallest (423-510 Mb) while the largest one is finger millet (1.5 Gb). Recent advances in phenotyping and genomics technologies, together with available germplasm diversity, could be utilized in small millets improvement. This review provides a comprehensive insight into the importance of small millets, the global status of their germplasm, diversity, promising germplasm resources, and breeding approaches (conventional and genomic approaches) to accelerate climate-resilient and nutrient-dense small millets for sustainable agriculture, environment, and healthy food systems.

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Characterization of metabolic network of oxalic acid biosynthesis through RNA seq data analysis of developing spikes of finger millet ( Eleusine coracana ): Deciphering the role of key genes involved in oxalate formation in relation to grain calcium accumulation

Cited by 21 publications

References 40 publications

High and low oxalate content in spinach: an investigation of accumulation patterns

High and low oxalate content in spinach: an investigation of accumulation patterns

Nutrients Anti-Nutrients Interaction in Millets and its Effect on Malnutrition

Genetic and genomic resources, and breeding for accelerating improvement of small millets: current status and future interventions

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