Characterization of metachronal wave of beating cilia on frog's palate epithelium in tissue culture.

Eshel, Dan; Priel, Zvi

doi:10.1113/jphysiol.1987.sp016597

Cited by 37 publications

(22 citation statements)

References 8 publications

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“…Therefore, uncorrelated fluctuations in ciliary frequency must lead to uncertainty in the phase shift. It was shown that even when the frequency is constant in time, quite strong fluctuations in phase do occur [Eshel and Priel, 1987;Ovadyahu and Priel, 1989;Gheber andPriel, 1989, 1990al. The results of the present work support those findings.…”

Section: Correlation Between Beating Ciliamentioning

confidence: 99%

Metachronal activity of cultured mucociliary epithelium under normal and stimulated conditions

Gheber

Priel

1994

Cell Motil. Cytoskeleton

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In the present work we measured in real time the metachronism and degree of correlation between beating cilia from cultured mucociliary epithelium. The method is based on simultaneous measurement of ciliary beat frequency, phase shifts, and correlation factors in two directions: parallel and perpendicular to the effective stroke direction (ESD). From the phase shifts the lengths of wave components, and consequently the metachronal wavelength and direction, were evaluated. On active ciliary areas of cultured frog esophagus under normal conditions, a relatively high degree of correlation is observed, but cilia are more correlated in direction parallel to ESD which is also the direction of the mucus propulsion. The length of the wave component parallel to ESD is more than twice as large as that of the perpendicular component. The metachronal wavelength was found to be in the range of 5-9 microns, and the direction of the wave propagation was in the range of 90 degrees-125 degrees clockwise to the ESD. When ciliary beat frequency was rapidly increased by extracellular ATP or acetylcholine, only minor effects were observed on the degree of correlation between beating cilia. The length of the wave component parallel to ESD showed the most dramatic effect increasing up to tenfold. The perpendicular to ESD component was not affected by the stimulation. Consequently, the metachronism became more laeoplectic with the angle between the ESD and the wave directions decreasing by 10 degrees-30 degrees, and the metachronal wavelength remained unaltered.

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Section: Correlation Between Beating Ciliamentioning

confidence: 99%

Metachronal activity of cultured mucociliary epithelium under normal and stimulated conditions

Gheber

Priel

1994

Cell Motil. Cytoskeleton

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“…Patches are distinct regions of ciliary activity each separated from one-another, and spread over ciliary epithelium lining various organs of the body. These patches of cilia span some few micrometers (Eshel and Priel [17]). It appears that in experimental studies, coordination of cilia has not been seen to exceed distances greater than a few micrometers.…”

Section: Introductionmentioning

confidence: 99%

Effect of bunching of cilia and their interplay on muco-ciliary transport

Roy

Sivathanu

Das

2013

Computers in Biology and Medicine

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Cilia are hair-like organelles projecting from a eukaryotic cell, used either for locomotion or as sensors. Cilia commonly occur in patches. To take this into consideration, we represent cilia in multiple patches, instead of the conventional 'dense mat' representation. We focus on the combined action and interplay of these patches. The effects of varying the frequency, spacing and phase lag of the beating of one cilia bunch with respect to the beating of adjacent patches are studied. We model the Airway Surface Liquid (ASL) as a three-layer structure. The possibility of an optimum frequency of beating is noted and the change of mucous flow under different spacing and phase differences are observed.

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“…Simultaneous measurements of scattered light from two points on the monolayer of ciliary epithelium was performed as previously described (Eshel and Priel, 1987). This method enables the measurement of cdliary beat frequency, and other important parameters of ciliary activity such as metachronal wavelength and velocity (Gheber and Priel, 1989Priel, 1987).…”

Section: Measurements Of Ciliary Beatingmentioning

confidence: 99%

Extracellular ATP induces hyperpolarization and motility stimulation of ciliary cells

Tarasiuk

Bar-Shimon

Gheber

et al. 1995

Biophysical Journal

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Cellular membrane potential and ciliary motility were examined in tissues cultures prepared from frog palate and esophagus epithelia. Addition of micromolar concentrations of extracellular ATP caused membrane hyperpolarization and enhanced the beat frequency. These two effects of ATP were 1) dose dependent, reaching a maximum at 10 microM ATP; 2) dependent on the presence of extracellular Ca2+ or Mg2+; 3) insensitive to inhibitors of voltage-gated calcium channels; 4) abolished after depleting the intracellular Ca2+ stores with thapsigargin; 5) attenuated by quinidine (1 mM), Cs+ (5-20 mM), and replacement of extracellular Na+ by K+; 6) insensitive to charybdotoxin (5-20 nM), TEA (1-20 microM), and apamin (0.1-1 microM); 7) independent of initial membrane potential; and 8) unaffected by amiloride. In addition, extracellular ATP induced an appreciable rise in intracellular Ca2+. Addition of thapsigargin caused an initial enhancement of the ciliary beat frequency and membrane hyperpolarization. These results strongly suggest the involvement of calcium-dependent potassium channels in the response to ATP. The results show that moderate hyperpolarization is closely associated with a sustained enhancement of ciliary beating by extracellular ATP.

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Characterization of metachronal wave of beating cilia on frog's palate epithelium in tissue culture.

Cited by 37 publications

References 8 publications

Metachronal activity of cultured mucociliary epithelium under normal and stimulated conditions

Metachronal activity of cultured mucociliary epithelium under normal and stimulated conditions

Effect of bunching of cilia and their interplay on muco-ciliary transport

Extracellular ATP induces hyperpolarization and motility stimulation of ciliary cells

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