Characterization of Microsatellites in the IGF-2 and GH Genes of Asian Seabass (Lates calcarifer)

Yue, Gen Hua; Li, Yang; Orbán, László

doi:10.1007/s101260000043

Cited by 54 publications

(29 citation statements)

References 12 publications

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“…Primers were designed for 306 microsatellites. Together with 18 microsatellites from genes (Yue et al 2001) and ESTs ) and 76 microsatellites published previously (Yue et al 2002;Sim and Othman 2005;Zhu et al 2006), a total of 400 primer pairs were tested on the three parents of the mapping panel containing two full-sib families to check the informativeness of markers for mapping. In one full-sib family, 194 markers were informative, while in another full-sib family, 184 markers were informative.…”

Section: Resultsmentioning

confidence: 99%

A Microsatellite Linkage Map of Barramundi, Lates calcarifer

Wang

Zhu

et al. 2007

Genetics

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Barramundi (Lates calcarifer) is an important farmed marine food fish species. Its compact genome ($700 Mb) is among the smallest genomes of food fish species. We established a first-generation genetic linkage map of Barramundi with a mapping panel containing three parents (two males and one female) and 93 progeny. A total of 240 microsatellite markers were mapped into 24 linkage groups. Among these markers, 10 were located in ESTs and known genes. The total lengths of the female and male maps were 873.8 and 414.5 cM with an average marker spacing of 6.20 and 4.70 cM, respectively. Comparing the flanking sequences of the 240 Barramundi microsatellites with the assembled whole-genome sequences of Tetraodon nigrovidiris revealed 55 homologous sequences located in 19 of the 21 chromosomes of T. nigrovidiris. The map will not only enable the mapping of quantitative trait loci, but also provide new resources for understanding the evolution of fish genomes.

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Section: Resultsmentioning

confidence: 99%

A Microsatellite Linkage Map of Barramundi, Lates calcarifer

Wang

Zhu

et al. 2007

Genetics

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“…Fish cell lines do not always contain the same number of chromosomes as the intact host species (Hsu, 1973). To confirm the cell lines originated from sea bass, PCR reactions with three microsatellite markers (Yue et al, 2001(Yue et al, , 2002 were performed. PCR products of expected size from tissues of sea bass and cell line were obtained from all the three markers used in this study.…”

Section: Discussionmentioning

confidence: 99%

Establishment and characterization of India's first marine fish cell line (SISK) from the kidney of sea bass (Lates calcarifer)

Hameed¹,

Parameswaran²,

Shukla

et al. 2006

Aquaculture

104

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A continuous cell line (SISK) from kidney of sea bass, Lates calcarifer, has been established and characterized. The cell line was maintained in Leibovitz' L-15 supplemented with 15% fetal bovine serum. This cell line has been subcultured more than 100 times over a period of 2 years. The SISK cell line consists of predominantly of epithelial-like cells. These cells showed strong positive for epithelial markers such as cytokeratin 19 and pancytokeratin. The cells were able to grow at temperature between 25 and 32°C with optimum temperature of 28°C. The growth rate of sea bass kidney cells increased as the FBS proportion increased from 2% to 20% at 28°C with optimum growth at the concentrations of 15% or 20% FBS. The distribution of chromosome number was 30 to 56 with a modal peak at 48 chromosomes. Polymerase chain reaction products were obtained from SISK cells and tissues of sea bass with primer sets of microsatellite markers of sea bass. Five fish viruses were tested on this cell line to determine its susceptibility to these viruses and this was found to be susceptible to MABV NC1 and nodavirus, and the infection was confirmed by RT-PCR and CPE. This suggests that the SISK cell line has good potential for the isolation of various fish viruses. This cell line has been shown to be susceptible to bacterial extracellular products. The SISK cell line is the India's first marine fish cell line.

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“…Virus source used in this study was diseased juveniles of marine sea bass fish species, determined previously as PCR positive for viral nervous necrosis (VNN) by using IQ2000 kit. This virus was collected from Malaysia during disease outbreak with mass mortality in (2002) 2008 and was diagnosed as VNN by RT-PCR analysis of coat protein gene T4 region. Whole brain and eyes for diseased juveniles were homogenized (Heidolph DIAX 900, Kelheim, Germany) with 9 vol of Hanks balanced salt solution (HBSS, Nissui Co. Ltd., Tokyo, Japan) and a small amount of sea sand under iced conditions, and then centrifuged at 1200×g for 10 min (4°C).…”

Section: Methodsmentioning

confidence: 99%

Development and partial characterization of new marine cell line from brain of Asian sea bass Lates calcarifer for virus isolation

Hasoon

Daud

Abdullah³

et al. 2010

In Vitro Cell.Dev.Biol.-Animal

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A new cell line, Asian sea bass brain (ASBB), was derived from the brain tissue of Asian sea bass Lates calcarifer. This cell line was maintained in Leibovitz L-15 media supplemented with 10% fetal bovine serum (FBS). The ASBB cell line was subcultured more than 60 times over a period of 15 mo. The ASBB cell line consists predominantly of fibroblastic-like cells and was able to grow at temperatures between 20°C and 30°C with an optimum temperature of 25°C. The growth rate of these cells increased as the proportion of FBS increased from 2% to 20% at 25°C with optimum growth at the concentrations of 10% or 15% FBS. Polymerase chain reaction products were obtained from ASBB cells and tissues of sea bass with primer sets of microsatellite markers of sea bass. An isolate of piscine nodavirus from juveniles of marine fish species tested positive by IQ2000 kit for viral nervous necrosis detection and was examined for its infectivity to a fish cell line of ASBB. A marine fish betanodavirus was tested to determine the susceptibility of this new cell line in comparison with commercial highly permissive SSN-1 cells. The ASBB cell line was found to be susceptible to nodavirus (RGNNV genotype), and the infection was confirmed by comparison cytopathic effect (CPE) with commercial SSN-1 and reverse transcriptase-polymerase chain reaction. A nodavirus was further elucidated by electron microscopy, and the virus tested was shown to induce CPE on ASBB cells with significant high titer. This suggests that the ASBB cell line has good potential for the isolation of fish viruses.

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Characterization of Microsatellites in the IGF-2 and GH Genes of Asian Seabass (Lates calcarifer)

Cited by 54 publications

References 12 publications

A Microsatellite Linkage Map of Barramundi, Lates calcarifer

A Microsatellite Linkage Map of Barramundi, Lates calcarifer

Establishment and characterization of India's first marine fish cell line (SISK) from the kidney of sea bass (Lates calcarifer)

Development and partial characterization of new marine cell line from brain of Asian sea bass Lates calcarifer for virus isolation

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