Characterization of miRNAs and their target genes in He-Ne laser pretreated wheat seedlings exposed to drought stress

Qiu, Zongbo; He, Yanyan; Zhang, Yimeng; Guo, Junli; Wang, Li

doi:10.1016/j.ecoenv.2018.08.077

Cited by 28 publications

(20 citation statements)

References 43 publications

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“…The miRNAs Sto-miR398b and Sto-miR5225 were co-downregulated in MDT and SDT. The downregulation of miR398 in S. tonkinensis was consistent with results reported in tobacco and wheat but was not consistent with results reported in M. truncatula and wild emmer wheat [ 62 , 63 ]. miR5225 is induced by drought in drought-tolerant apple trees [ 64 ].…”

Section: Discussionsupporting

confidence: 50%

Integrated transcriptome and small RNA sequencing analyses reveal a drought stress response network in Sophora tonkinensis

et al. 2021

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Background Sophora tonkinensis Gagnep is a traditional Chinese medical plant that is mainly cultivated in southern China. Drought stress is one of the major abiotic stresses that negatively impacts S. tonkinensis growth. However, the molecular mechanisms governing the responses to drought stress in S. tonkinensis at the transcriptional and posttranscriptional levels are not well understood. Results To identify genes and miRNAs involved in drought stress responses in S. tonkinensis, both mRNA and small RNA sequencing was performed in root samples under control, mild drought, and severe drought conditions. mRNA sequencing revealed 66,476 unigenes, and the differentially expressed unigenes (DEGs) were associated with several key pathways, including phenylpropanoid biosynthesis, sugar metabolism, and quinolizidine alkaloid biosynthesis pathways. A total of 10 and 30 transcription factors (TFs) were identified among the DEGs under mild and severe drought stress, respectively. Moreover, small RNA sequencing revealed a total of 368 miRNAs, including 255 known miRNAs and 113 novel miRNAs. The differentially expressed miRNAs and their target genes were involved in the regulation of plant hormone signal transduction, the spliceosome, and ribosomes. Analysis of the regulatory network involved in the response to drought stress revealed 37 differentially expressed miRNA-mRNA pairs. Conclusion This is the first study to simultaneously profile the expression patterns of mRNAs and miRNAs on a genome-wide scale to elucidate the molecular mechanisms of the drought stress responses of S. tonkinensis. Our results suggest that S. tonkinensis implements diverse mechanisms to modulate its responses to drought stress.

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Section: Discussionsupporting

confidence: 50%

Integrated transcriptome and small RNA sequencing analyses reveal a drought stress response network in Sophora tonkinensis

et al. 2021

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“…Recently, Tyagi et al [37] supported the participation of TaCu-ZnSOD genes in the response of wheat to biotic and abiotic stress by analyzing their expression after the infection of plants with Blumeria graminis and Puccinia striiformis and in conditions of heat, drought, and salinity. With regard to Li et al's [33] research proving the participation of miR398 in the regulation of the CSD genes expression, the role of this molecule in modulating wheat response to oxidative stress, and thus in the adaptation to unfavorable environmental conditions, has been recognized [23,30,32,[38][39][40][41][42]. However, miR398 in wheat has been identified mostly in response to abiotic stresses such as cold [23,32], injury [23], and drought [32,42].…”

Section: Discussionmentioning

confidence: 99%

“…With regard to Li et al's [33] research proving the participation of miR398 in the regulation of the CSD genes expression, the role of this molecule in modulating wheat response to oxidative stress, and thus in the adaptation to unfavorable environmental conditions, has been recognized [23,30,32,[38][39][40][41][42]. However, miR398 in wheat has been identified mostly in response to abiotic stresses such as cold [23,32], injury [23], and drought [32,42]. The results of our research suggest that miR398 is also involved in wheat responses to infections with pathogenic F. culmorum strains, as well as inoculation with symbiotic Trichoderma fungi.…”

Section: Discussionmentioning

confidence: 99%

Expression Patterns of miR398, miR167, and miR159 in the Interaction between Bread Wheat (Triticum aestivum L.) and Pathogenic Fusarium culmorum and Beneficial Trichoderma Fungi

et al. 2021

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Bread wheat (Triticum aestivum L.) is an agronomically significant cereal cultivated worldwide. Wheat breeding is limited by numerous abiotic and biotic stresses. One of the most deleterious factors is biotic stress provoked by the Fusarium culmorum fungus. This pathogen is a causative agent of Fusarium root rot and Fusarium head blight. Beneficial fungi Trichoderma atroviride and T. cremeum are strong antagonists of mycotoxigenic Fusarium spp. These fungi promote plant growth and enhance their tolerance of negative environmental conditions. The aim of the study was to determine and compare the spatial (in above- and underground organs) and temporal (early: 6 and 22 hpi; and late: 5 and 7 dpi reactions) expression profiles of three mature miRNAs (miR398, miR167, and miR159) in wheat plants inoculated with two strains of F. culmorum (KF846 and EW49). Moreover, the spatial expression patterns in wheat response between plants inoculated with beneficial T. atroviride (AN35) and T. cremeum (AN392) were assessed. Understanding the sophisticated role of miRNAs in wheat–fungal interactions may initiate a discussion concerning the use of this knowledge to protect wheat plants from the harmful effects of fungal pathogens. With the use of droplet digital PCR (ddPCR), the absolute quantification of the selected miRNAs in the tested material was carried out. The differential accumulation of miR398, miR167, and miR159 in the studied groups was observed. The abundance of all analyzed miRNAs in the roots demonstrated an increase in the early and reduction in late wheat response to F. culmorum inoculation, suggesting the role of these particles in the initial wheat reaction to the studied fungal pathogen. The diverse expression patterns of the studied miRNAs between Trichoderma–inoculated or F. culmorum–inoculated plants and control wheat, as well as between Trichoderma–inoculated and F. culmorum–inoculated plants, were noticed, indicating the need for further analysis.

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“…Additionally, in rice, it was evidenced that miR159 targets Cu/ZnSOD (At5g18100-CSD3) (Li et al, 2010). Another group of scientists has shown that CSD is a potential targets of miR398 in wheat seedlings exposed to drought (Qiu et al, 2018). Cytosolic CSD1/2 was found to be targeted by miR398 in wheat in response to the fungal attack by Fusarium graminearum which causes Fusarium head blight disease (Biselli et al, 2018).…”

Section: Involvement Of Plant Mirnas In Genotoxic Stress Tolerancementioning

confidence: 99%

Tiny Yet Indispensable Plant MicroRNAs Are Worth to Explore as Key Components for Combating Genotoxic Stresses

Chowdhury

Basak

2019

Front. Plant Sci.

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Plants being sessile are always exposed to various stresses including biotic and abiotic stresses. Some of these stresses are genotoxic to cells causing DNA damage by forming lesions which include altered bases, cross-links, and breaking of DNA strands, which in turn hamper the genomic integrity. In order to survive through all these adverse conditions, plants have evolved different DNA repair mechanisms. As seen from the mammalian system and different human diseases, various microRNAs (miRNAs) can target the 3′-untranslated region of mRNAs that code for the proteins involved in DNA repair pathways. Since miRNAs play an important role in plant cells by regulating various metabolic pathways, it can also be possible that miRNAs play an important role in DNA repair pathways too. However, till date, only a handful of plant miRNAs have been identified to play important role in combating genotoxic stresses in plants. Limitation of information regarding involvement of miRNAs in DNA repair as well as in ROS scavenging prompted us to gather information about plant miRNAs specific for these tasks. This mini-review aims to present pertinent literature dealing with different genotoxic stresses that cause genome instability as well as plant specific responses to survive the damage. This is intertwined with the involvement of miRNAs in genotoxic stress in plants, challenges of applying miRNAs as a tool to combat DNA damage along with ways to overcome these challenges, and finally, the future prospective of these understudied aspects.

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Characterization of miRNAs and their target genes in He-Ne laser pretreated wheat seedlings exposed to drought stress

Cited by 28 publications

References 43 publications

Integrated transcriptome and small RNA sequencing analyses reveal a drought stress response network in Sophora tonkinensis

Integrated transcriptome and small RNA sequencing analyses reveal a drought stress response network in Sophora tonkinensis

Expression Patterns of miR398, miR167, and miR159 in the Interaction between Bread Wheat (Triticum aestivum L.) and Pathogenic Fusarium culmorum and Beneficial Trichoderma Fungi

Tiny Yet Indispensable Plant MicroRNAs Are Worth to Explore as Key Components for Combating Genotoxic Stresses

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