Characterization of Modified Magnetite Nanoparticles for Albumin Immobilization

Abstract

Magnetite Fe3O4 nanoparticles (NPs) were prepared by chemical coprecipitation method. Silica-coated magnetite NPs were prepared by sol-gel reaction, subsequently coated with 3-aminopropyltriethoxysilane (APTES) via silanization reaction, and then were activated with 2,4,6-trichloro-1,3,5-triazine (TCT) and covalently immobilized with bovine serum albumin (BSA). The size and structure of the particles were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), and dynamic light scattering (DLS) techniques. The immobilization was confirmed by Fourier transform infrared spectroscopy (FT-IR). XRD analysis showed that the binding process has not done any phase change to Fe3O4. The immobilization time for this process was 4 h and the amount of immobilized BSA for the initial value of 1.05 mg BSA was about 120 mg/gr nanoparticles. Also, the influences of three different buffer solutions and ionic strength on covalent immobilization were evaluated.

Modeling of Magnetite Nanoparticles Behavior under Conditions of Microcirculation and Analysis of In Vivo Toxicity

“…Hence, high aggregation stability of magnetite and aminated magnetite was confirmed under conditions close to physiological ones, which was in line with previous reports [3,4].…”

“…Aggregation stability of NP in biological specimens is the main problem in creation of ACNM containing vectors and therapeutic agents [3,11]. The behavior and stability of magnetite NP and aminated NP in microcapillaries were studied in a microbioreactor.…”

“…Magnetite-based ACNM promptly react to changes in the temperature, light, pH, magnetic field, and ultrasonic exposure [3,11].…”

Modification of magnetic nanoparticle lipase designs for biodiesel production from palm oil

“…The FT-IR spectra of T. lanuginosus lipase showed signature peaks at 1643.99 cm −1 for the carbonyl amide group (O_C stretching vibration) and 1557. 26 …”

“…The higher catalytic performance of the biocatalysts prepared on Fe 3 O 4 -APTES matrix could be related to the silane coating i.e. APTES on the surface of magnetic support, which provides a high density of surface functional groups for coupling [26]. Silane coatings enhance enzyme binding to the support and improve enzyme stability [27].…”