Characterization of molecular species of collagen in muscles of Japanese amberjack, Seriola quinqueradiata

Nishimoto, Maki; Mizuta, Shoshi; Yoshinaka, Ryo

doi:10.1016/s0308-8146(03)00183-3

Cited by 13 publications

(11 citation statements)

References 18 publications

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“…We speculated that the major collagen of the scallop mantle had a similar role to the mammalian type I collagen, and that the minor collagen of the scallop to the type Ⅴcollagen in the tissue. However, the amino acid compositions of both major and minor collagens were comparable to those of the corresponding vertebrate collagens (Nishimoto et al, 2004;Nishimoto, sakamoto, Mizuta, and Yoshinaka, 2005).…”

Section: Extraction Of the Collagen And Its Puritymentioning

confidence: 85%

“…And indeed there was a difference between these two, as seen in Table 1. As well as type I and type Ⅴ collagens, major and minor collagens have been fractionated from various marine animal tissues (Kimura et al, 1987(Kimura et al, , 1988Sato et al, 1988Sato et al, , 1989Nishimoto et al, 2004). There is a report on marine invertebrate collagen which was shown to have charac-teristics similar to mammalian type Ⅴcollagen, and workers have succeeded in separating those molecular species (Mizuta, Miyagi, and Yoshinaka, 2005;Sivakumar, suguna, and Chandrakasan, 2000).…”

Section: Extraction Of the Collagen And Its Puritymentioning

confidence: 99%

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Characterization of molecular species of collagen in scallop mantle

2007

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Pepsin-solubilized collagen (SMPC) extracted from scallop mantle was first subjected to amino acid analysis and molecular weight determination. The SMPC was rich in hydroxylysine. After salting out, the SMPC showed two different profiles in molecules, amino acid and peptide map. Those two different collagen molecules had different uronic acid content and gel-forming ability. SMPC may be applicable to variety of usage including functional foods.

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Section: Extraction Of the Collagen And Its Puritymentioning

confidence: 85%

Section: Extraction Of the Collagen And Its Puritymentioning

confidence: 99%

Characterization of molecular species of collagen in scallop mantle

2007

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“…The present study demonstrates that the digestive tract has a higher content of collagen (2%) than ordinary (0.3%) and dark muscles (0.8%) of the Japanese amberjack (Nishimoto et al. 2004).…”

Section: Discussionmentioning

confidence: 49%

Characterization and Comparison of Collagens Extracted From the Digestive Tract and Skin of a Japanese Amberjack Seriola Quinqueradiata

Nishimoto

Mizuta

Yoshinaka

et al. 2009

Journal of Food Biochemistry

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Collagen was extracted from the digestive tract and skin of a Japanese amberjack (Seriola quinqueradiata) by acid extraction and limited pepsin digestion. The amounts of collagen solubilized from the digestive tract were smaller than those from the skin. Based on the solubility in NaCl solution, electrophoretic and peptide map patterns, and amino acid composition, the main digestive tract collagen was identified as type I, having characteristics different from those of the body wall collagen in cyclostome intestine. Further, the degree of hydroxylation of prolyl and lysyl residues in the type I collagen of the digestive tract is significantly higher than that of the skin. Collagen preparations from the digestive tract have a higher ratio of type V collagen than those from the skin. Hence, the digestive tract collagen differs from that in the skin in the degree or property of intermolecular crosslinking, posttranslational modification, and molecular species composition. PRACTICAL APPLICATIONS Partial hydrolyzate of gelatin, in other word collagen peptide, has gained popularity as a food ingredient, as it has been suggested to have health benefits, such as improvement of skin and joint conditions. Recently, attention toward collagen derived from marine origin such as fish skin increased because of the outbreak of bovine spongiform encephalopathy. Large amounts of the digestive tract, stomach, intestine and adhesion tissues are generated by fishery industries and most of them are by‐products of low value. Although these organs are also rich in collagen, the collagen in fish digestive tract has not been characterized. The present study demonstrates that the collagen in digestive tract differs from the skin collagen in the solubility, posttranslational modification and molecular species composition. These facts suggest that modified collagen peptides might be obtained from the digestive tract.

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“…The amino acid composition of the oyster major collagen was essentially similar to that of the major collagen from the mantle of the pearl oyster Pinctada martensii reported by Kimura et al . 29 In addition, the major collagen showed compositional features similar to fish type V/XI collagens, [30][31][32][33][34] squid minor collagen (Type SQ-II), 6 and other invertebrate collagens, 35,36 especially in the relatively low content of alanine and high content of hydroxylysine ( Table 1).…”

Section: Resultsmentioning

confidence: 95%

“…2a. The fractions comprising the latter part of the peak (fractions [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35] showed collagenous bands, a 1 and a 2, in SDS-PAGE (Fig. 2b).…”

Section: Resultsmentioning

confidence: 99%

Characterization of the quantitatively major collagen in the mantle of oyster Crassostrea gigas

2005

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A quantitatively major collagen was isolated from the pepsin-solubilized collagen preparation of the mantle by differential salt precipitation and phosphocellulose column chromatography, and its constituent a components ( a 1 and a 2) were purified by phosphocellulose column chromatography. The subunits were demonstrated to be genetically distinct from each other by peptide mapping and amino acid analysis. The amino acid composition calculated from those of the a 1 and a 2 components in 2 : 1 ratio coincided well with that of the major collagen from the mantle. These results suggest that the major collagen in the mantle of the oyster may have a heterotrimer structure ( a 1) 2 a 2.

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Characterization of molecular species of collagen in muscles of Japanese amberjack, Seriola quinqueradiata

Cited by 13 publications

References 18 publications

Characterization of molecular species of collagen in scallop mantle

Characterization of molecular species of collagen in scallop mantle

Characterization and Comparison of Collagens Extracted From the Digestive Tract and Skin of a Japanese Amberjack Seriola Quinqueradiata

Characterization of the quantitatively major collagen in the mantle of oyster Crassostrea gigas

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