Characterization of mRNA profiles of the exosome-like vesicles in porcine follicular fluid

Matsuno, Yuta; Kanke, Takuya; Maruyama, Natsumi; Fujii, Wataru; Naito, Kunihiko; Sugiura, Koji

doi:10.1371/journal.pone.0217760

Cited by 53 publications

(44 citation statements)

References 57 publications

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“…The study in equine follicular fluid showed that contains microvesicle that carries proteins and miRNAs and some of these miRNAs are present in the surrounding granulosa and cumulus cells suggesting the presence of transfer of bioactive material by microvesicles and exosomes within the follicular microenvironment [72]. The presence of miRNAs in extra cellular vesicles of bovine [28,73], human [74] and porcine [75] follicular fluids indicated the potential roles of EV-mediated miRNAs in folliculogenesis. The expression patterns of the EV-mediated miRNAs depends on follicle size [76] and / or reproductive cycles [77].…”

Section: The Role Of Extracellular Vesicles-mediated Mirnas In Femalementioning

confidence: 99%

The Role of MicroRNAs in Mammalian Fertility: From Gametogenesis to Embryo Implantation

Salilew‐Wondim

Gebremedhn

Hoelker

et al. 2020

IJMS

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The genetic codes inscribed during two key developmental processes, namely gametogenesis and embryogenesis, are believed to determine subsequent development and survival of adult life. Once the embryo is formed, its further development mainly depends on its intrinsic characteristics, maternal environment (the endometrial receptivity), and the embryo–maternal interactions established during each phase of development. These developmental processes are under strict genetic regulation that could be manifested temporally and spatially depending on the physiological and developmental status of the cell. MicroRNAs (miRNAs), one of the small non-coding classes of RNAs, approximately 19–22 nucleotides in length, are one of the candidates for post-transcriptional developmental regulators. These tiny non-coding RNAs are expressed in ovarian tissue, granulosa cells, testis, oocytes, follicular fluid, and embryos and are implicated in diverse biological processes such as cell-to-cell communication. Moreover, accumulated evidences have also highlighted that miRNAs can be released into the extracellular environment through different mechanisms facilitating intercellular communication. Therefore, understanding miRNAs mediated regulatory mechanisms during gametogenesis and embryogenesis provides further insights about the molecular mechanisms underlying oocyte/sperm formation, early embryo development, and implantation. Thus, this review highlights the role of miRNAs in mammalian gametogenesis and embryogenesis and summarizes recent findings about miRNA-mediated post-transcriptional regulatory mechanisms occurring during early mammalian development.

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Section: The Role Of Extracellular Vesicles-mediated Mirnas In Femalementioning

confidence: 99%

The Role of MicroRNAs in Mammalian Fertility: From Gametogenesis to Embryo Implantation

Salilew‐Wondim

Gebremedhn

Hoelker

et al. 2020

IJMS

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show abstract

“…Their later findings indicated a possible role of exosomes from FF in the regulation of follicle maturation [ 139 ]. CD63-positive EV-mediated cell-cell communication within the follicle was also confirmed in cattle [ 140 ] and in pigs [ 141 ]. Later, the uptake of EVs (CD63-positive) by bovine granulosa and cumulus cells was documented, and EVs were identified in cumulus cells and in transzonal projections [ 142 ].…”

Section: Tetraspanins In Evs Of the Female And Male Reproductive Smentioning

confidence: 90%

Tetraspanins, More than Markers of Extracellular Vesicles in Reproduction

Jankovičová

Sečová

Michalková

et al. 2020

IJMS

142

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The participation of extracellular vesicles in many cellular processes, including reproduction, is unquestionable. Although currently, the tetraspanin proteins found in extracellular vesicles are mostly applied as markers, increasing evidence points to their role in extracellular vesicle biogenesis, cargo selection, cell targeting, and cell uptake under both physiological and pathological conditions. In this review, we bring other insight into the involvement of tetraspanin proteins in extracellular vesicle physiology in mammalian reproduction. We provide knowledge regarding the involvement of extracellular vesicle tetraspanins in these processes in somatic cells. Furthermore, we discuss the future direction towards an understanding of their functions in the tissues and fluids of the mammalian reproductive system in gamete maturation, fertilization, and embryo development; their involvement in mutual cell contact and communication in their complexity.

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“…Other eukaryotic cells can secrete these membrane vesicles, and the quality of exosomes is dependent on the state of the releasing cell [33]. A recent study has suggested that other cells in follicular fluid-like oocytes or theca cells may secrete exosomal-like vesicles, but that the majority originated from mural GCs [34]. Furthermore, exosomes carry proteins and MicroRNAs (miRNAs) [5].…”

Section: Characteristics Of Exosomes and Cell Stressmentioning

confidence: 99%

Ovarian follicular cells - living in the shadow of stemness cellular competence

Moncrieff

Mozdziak

Ješeta

et al. 2019

Medical Journal of Cell Biology

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Granulosa cells (GCs) are a major component found in ovarian follicular fluid among oocytes, theca cells, and ovarian surface epithelial (OSE) cells. GCs are steroidogenic and have morphological functions that are important for the development of the follicular follicle. The follicle protects the developing female egg. GCs are also essential for the maturation of the female germ cell. Stem cell properties have been found in luteinised GCs and in vivo cultures have the potential to differentiate to become cells found outside of the ovary. Both three-dimensional (3D) culturing and mouse embryonic fibroblast (MEF) medium have been used to help improve the culturing lifespan of GCs so that their profound proliferation and differentiation capabilities can be studied. Small RNAs called MicroRNAs (miRNAs) are released from exosomes originating from GCs, and they are involved in transforming growth factor (TGF)-β signalling, follicle-stimulating hormone, hormone-related miRNAs, and apoptosis-related pathway. Finding the miRNAs involved in these pathways, and the mechanisms controlling GCs are important to treating conditions like polycystic ovary syndrome (PCOS), and creating new drug therapies. Besides GCs, ovarian stem cells (OSCs) were discovered in the OSE, and they are believed to be derived from very small embryonic stem cells (VSELs). Transplanting blood mononuclear cells, cell Hormone Therapy (cHT) with bone marrow stem cell supplement and increasing vitamin C levels are all therapies currently being researched into that involve GCs to combat the effects of aging and infertility.Running title: Stemness of ovarian follicular cells

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Characterization of mRNA profiles of the exosome-like vesicles in porcine follicular fluid

Cited by 53 publications

References 57 publications

The Role of MicroRNAs in Mammalian Fertility: From Gametogenesis to Embryo Implantation

The Role of MicroRNAs in Mammalian Fertility: From Gametogenesis to Embryo Implantation

Tetraspanins, More than Markers of Extracellular Vesicles in Reproduction

Ovarian follicular cells - living in the shadow of stemness cellular competence

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