Characterization of NDM-7 Carbapenemase-Producing <i>Escherichia coli</i> Isolates in the Arabian Peninsula

Pál, Tibor; Ghazawi, Akela; Darwish, Dania; Villa, Laura; Carattoli, Alessandra; Hashmey, Rayhan; AlDeesi, Zulfa O.; Jamal, Wafaa; Rotimi, Vincent O.; Al-Jardani, Amina; Al-Abri, Seif; Sonnevend, Ágnes

doi:10.1089/mdr.2016.0216

Cited by 38 publications

(28 citation statements)

References 30 publications

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“…In E. coli the predominant NDM variant was found to be bla NDM−1 , followed by bla NDM−4 , bla NDM−5 , and bla NDM−7 (Figure 3 ). Although this is not first description of these NDM variants being produced by E. coli (Zhang et al, 2013 ; Qin et al, 2016 ; Zhu et al, 2016 ; Pál et al, 2017 ). Moreover, in these strains existence of NDM and its variants, with CMY, OXA, SHV, and VIM variants and other resistant determinants are documented.…”

Section: Discussionmentioning

confidence: 93%

Occurrence of blaNDM Variants Among Enterobacteriaceae From a Neonatal Intensive Care Unit in a Northern India Hospital

et al. 2018

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Carbapenem-resistance among enterobacteriaceae has become a global health concern. The objective of this study was to understand NDM producing enterobacteriaceae and their genetic basis of resistance, spreading in neonatal intensive care unit. Carbapenem resistant NDM producing enterobacteriaceae isolates were recovered from rectal swab and blood sample of infants admitted in NICU. These were determined by using Carba-NP test. All isolates were identified using BD PhoenixTM−100 and MICs were determined by broth microdilution method. The blaNDM and associated resistant markers were checked by PCR followed by sequencing. Moreover, ERIC-PCR and genetic environment of blaNDM gene were also performed for the analysis of clonal relationship and genetic surrounding of the strains. We characterized 44 isolates with blaNDM variants in Escherichia coli (45.5%), Klebsiella pneumoniae (40.9%), Citrobacter freundii (4.5%), Citrobacter braakii (2.3%), Klebsiella oxytoca (2.3%), Enterobacter cloacae (2.3%), Enterobacter aerogenes (2.2%) from NICU, showing resistance against all antibiotics except colistin and polymixin B. ISAba125 and bleomycin gene were found surrounding all blaNDM variants, besides class I integron on plasmid. (ERIC)-PCR data revealed non-clonal relatedness among most of the isolates. The transfer of resistant markers was confirmed by conjugation experiment. The PCR-based replicon typing was carried out using DNA of transconjugants. These isolates carried NDM-1 (20.45%), NDM-4 (36.36%), NDM-5 (38.64%), NDM-7 (4.55%), along with OXA, CMY, and SHV variants on conjugative plasmid of IncFIA, IncFIC, IncF, IncK, IncFIB, IncB/O, IncHI1, IncP, IncY, IncFIIA, IncI1, and IncN types. An increased number of carbapenem-resistant NDM producing enterobacteriaceae isolates recovered from NICU which is alarming signal for health workers and policy makers. Hence, it is utmost important to think about infection control measures.

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Section: Discussionmentioning

confidence: 93%

Occurrence of blaNDM Variants Among Enterobacteriaceae From a Neonatal Intensive Care Unit in a Northern India Hospital

et al. 2018

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“…In pJN05NDM-7, bla NDM-7 was preceded by IS 3000 -IS Aba125 -IS 5 in the upstream region and followed by ble-trpF-dsbC -IS 26 -Δ umuD in the downstream region. This bla NDM genetic structure was common in Enterobacteriaceae for the horizontal transfer of bla NDM ( Pal et al, 2017 ).…”

Section: Resultsmentioning

confidence: 99%

“…KX214670), and pABC133-NDM (Accession No. KX214671) ( Espedido et al, 2015 ; Pal et al, 2017 ). Sequence alignments revealed that pJN05NDM-7 was 100% identical to the previously described plasmid pKW53T-NDM of E. coli isolated in Kuwait (KW53T).…”

Section: Resultsmentioning

confidence: 99%

“…Escherichia coli isolates carrying bla NDM-7 belonging to different STs were sporadic reported worldwide ( Cuzon et al, 2013 ; Wang et al, 2016 ; Devanga Ragupathi et al, 2017 ; Pal et al, 2017 ; Espinal et al, 2018 ). According to previous reports, bla NDM-7 gene can be carried by several Enterobacter species and multiple types of plasmids including IncX3, IncF, and IncA/C groups, with sizes ranging from 37 to > 100 kb.…”

Section: Discussionmentioning

confidence: 99%

“…NDM-7, which differs from NDM-1 by two point mutations corresponding to amino acid substitutions, was described in 2013 with increased carbapenemase activity compared with NDM-1 ( Cuzon et al, 2013 ). bla NDM-7 is infrequently detected, and sporadic cases of infections due to bla NDM-7 -producing enterobacteria have been reported in France, India, the United States of America, and Japan ( Cuzon et al, 2013 ; Chen et al, 2015 ; Wang et al, 2016 ; Devanga Ragupathi et al, 2017 ; Pal et al, 2017 ; Sugawara et al, 2017 ; Espinal et al, 2018 ). In China, bla NDM-7 -producing E. coli ST131 was first reported in 2016; however, the genetic content of bla NDM-7 -harboring plasmids was not clearly described ( Wang et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Genotypic and Phenotypic Characterization of IncX3 Plasmid Carrying blaNDM-7 in Escherichia coli Sequence Type 167 Isolated From a Patient With Urinary Tract Infection

et al. 2018

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Infections due to New Delhi metallo-beta lactamase (NDM)-7-producing Escherichia coli are infrequent and sporadic. In this study, we report one case of recurrent urinary tract infection caused by blaNDM-7-producing E. coli belonging to phylogenetic group A, sequence type (ST) 167. In this study, we aimed to describe the genotype and phenotype of blaNDM-7-producing E. coli in China. The isolate exhibited resistance to β-lactam antimicrobials, trimethoprim-sulfamethoxazole, quinolones, and aminoglycosides. blaNDM-7 is located on a conjugative plasmid designated pJN05NDM-7 belonging to type IncX3. pJN05NDM-7 was fully sequenced and compared with all publicly available blaNDM-7-harboring plasmids. pJN05NDM-7 is almost identical to pKpN01-NDM7 and pKW53T, although the plasmids are geographically unrelated. The comparison of IncX3 plasmids harboring blaNDM in China showed high similarity, with genetic differences within insertion fragments. Notably, the differences in plasmids of animal and human origin were insignificant, because only one plasmid showed deletion inside the ISAba125 region compared with pJN05NDM7. Our study demonstrates that E. coli carrying IncX3 plasmids play an important role as a reservoir and in the spread of blaNDM. Further studies should be performed to control the dissemination of blaNDM among food animals.

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Plasmids Associated with Beta-Lactamases in Bacteria

Sami¹,

Shahid²,

Khan³

2022

Beta-Lactam Resistance in Gram-Negative Bacteria

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Characterization of NDM-7 Carbapenemase-Producing Escherichia coli Isolates in the Arabian Peninsula

Cited by 38 publications

References 30 publications

Occurrence of blaNDM Variants Among Enterobacteriaceae From a Neonatal Intensive Care Unit in a Northern India Hospital

Occurrence of blaNDM Variants Among Enterobacteriaceae From a Neonatal Intensive Care Unit in a Northern India Hospital

Genotypic and Phenotypic Characterization of IncX3 Plasmid Carrying blaNDM-7 in Escherichia coli Sequence Type 167 Isolated From a Patient With Urinary Tract Infection

Plasmids Associated with Beta-Lactamases in Bacteria

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