Characterization of NikA Histidine Kinase and Two Response Regulators with Special Reference to Osmotic Adaptation and Asexual Development in<i>Aspergillus nidulans</i>

Hagiwara, Daisuke; Mizuno, Takeshi; Abe, Keietsu

doi:10.1271/bbb.90063

Cited by 31 publications

(19 citation statements)

References 35 publications

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“…In A. nidulans deletion of the homologous nikA gene had severe consequences for the production and viability of asexual spores [14], [15], [16]. We therefore compared sporulation and conidial viability of the Δ tcsC mutant and its parental strain.…”

Section: Resultsmentioning

confidence: 99%

The Two-Component Sensor Kinase TcsC and Its Role in Stress Resistance of the Human-Pathogenic Mold Aspergillus fumigatus

Jacobsen

Broniszewska

et al. 2012

PLoS ONE

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Two-component signaling systems are widespread in bacteria, but also found in fungi. In this study, we have characterized TcsC, the only Group III two-component sensor kinase of Aspergillus fumigatus. TcsC is required for growth under hyperosmotic stress, but dispensable for normal growth, sporulation and conidial viability. A characteristic feature of the ΔtcsC mutant is its resistance to certain fungicides, like fludioxonil. Both hyperosmotic stress and treatment with fludioxonil result in a TcsC-dependent phosphorylation of SakA, the final MAP kinase in the high osmolarity glycerol (HOG) pathway, confirming a role for TcsC in this signaling pathway. In wild type cells fludioxonil induces a TcsC-dependent swelling and a complete, but reversible block of growth and cytokinesis. Several types of stress, such as hypoxia, exposure to farnesol or elevated concentrations of certain divalent cations, trigger a differentiation in A. fumigatus toward a “fluffy” growth phenotype resulting in white, dome-shaped colonies. The ΔtcsC mutant is clearly more susceptible to these morphogenetic changes suggesting that TcsC normally antagonizes this process. Although TcsC plays a role in the adaptation of A. fumigatus to hypoxia, it seems to be dispensable for virulence.

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Section: Resultsmentioning

confidence: 99%

The Two-Component Sensor Kinase TcsC and Its Role in Stress Resistance of the Human-Pathogenic Mold Aspergillus fumigatus

Jacobsen

Broniszewska

et al. 2012

PLoS ONE

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“…We previously demonstrated that NikA is essential for the growth inhibitory effect of fludioxonil (Hagiwara et al, 2007b) and DnikA cells show growth retardation on high osmolarity agar medium (Hagiwara et al, 2009). Thus, NikA was supposed to play a critical role in the response to these signals.…”

Section: Nika Is Involved In the Transcriptional Response To Fludioxomentioning

confidence: 99%

Transcriptional profiling for Aspergillus nidulans HogA MAPK signaling pathway in response to fludioxonil and osmotic stress

Hagiwara¹,

Asano²,

Marui³

et al. 2009

Fungal Genetics and Biology

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“…SakA was also shown to be involved in conidia viability in A. fumigatus , where the sakA mutant presented alterations in conidia germination in a nitrogen source-dependent manner [68]. The bZip-type transcription factor AtfA (putative ortholog of S. pombe Atf1), that functions downstream of the HogA MAPK in the course of fludioxonil and osmotic stress response, is also involved in conidia stress tolerance [58,59,69,70]. Also in A. fumigatus , mutations in MA21, a homolog of the S. cerevisiae transmembrane sensor kinase SHO gene, showed a reduction in growth and germination rates.…”

Section: Role Of the Osmotic Stress-response Pathway On Fungal Devmentioning

confidence: 99%

Role of the Osmotic Stress Regulatory Pathway in Morphogenesis and Secondary Metabolism in Filamentous Fungi

Duran

Cary

Calvo

2010

Toxins

121

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Environmental stimuli trigger an adaptative cellular response to optimize the probability of survival and proliferation. In eukaryotic organisms from mammals to fungi osmotic stress, mainly through the action of the high osmolarity glycerol (HOG) pathway, leads to a response necessary for adapting and surviving hyperosmotic environments. In this review we show that the osmoadaptative response is conserved but not identical in different fungi. The osmoadaptative response system is also intimately linked to morphogenesis in filamentous fungi, including mycotoxin producers. Previous studies indicate that the response to osmotic stress is also coupled to the biosynthesis of natural products, including mycotoxins.

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Characterization of NikA Histidine Kinase and Two Response Regulators with Special Reference to Osmotic Adaptation and Asexual Development inAspergillus nidulans

Cited by 31 publications

References 35 publications

The Two-Component Sensor Kinase TcsC and Its Role in Stress Resistance of the Human-Pathogenic Mold Aspergillus fumigatus

The Two-Component Sensor Kinase TcsC and Its Role in Stress Resistance of the Human-Pathogenic Mold Aspergillus fumigatus

Transcriptional profiling for Aspergillus nidulans HogA MAPK signaling pathway in response to fludioxonil and osmotic stress

Role of the Osmotic Stress Regulatory Pathway in Morphogenesis and Secondary Metabolism in Filamentous Fungi

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