Characterization of Novel Lactobacillus paracasei HY7017 Capable of Improving Physiological Properties and Immune Enhancing Effects Using Red Ginseng Extract

Abstract

Red ginseng has powerful potential for use as a prebiotic, but its use is limited due to its antibacterial activity. The aim of this study is to present panax ginseng’s endophytic lactic acid bacteria capable to overcome the antibacterial activity of red ginseng and improve their characteristic. Lactobacillus paracasei HY7017 (HY7017) was cultured in a medium supplemented with red ginseng. The probiotic properties and immune-enhancing effects of HY7017 were investigated in vitro and in vivo. HY7017 was proliferated strongly in RGE and had significantly improved properties compared with an L. paracasei type strain ATCC25302. HY7017 cultured in RGE-supplemented medium increased the production of nitric oxide, TNF-α, and IL-6 in macrophages, and increased IL-12 and IFN-γ secretion in splenocytes. Furthermore, HY7017 restored WBC counts, increased the amount of IL-2 and IFN-γ released, and enhanced the cytotoxicity of natural killer cells when orally administered to immunosuppressed mice. Moreover, HY7017 has properties that make it suitable as a probiotic, such as stability in the gastrointestinal tract and adhesion to Caco-2 cells. This study showed that HY7017 cultured with RGE may contribute to the development of probiotics to enhance immunity.

Immunostimulatory effects of dairy probiotic strains Bifidobacterium animalis ssp. lactis HY8002 and Lactobacillus plantarum HY7717

“…Spleen is a significant organ in terms of understanding immune cell subpopulations and their immune responses to antigens. Splenocytes include T and B cells, macrophages, and natural killer (NK) cells [ 34 ]. Their immune responses include antigen presentation, T lymphocyte activation, and B lymphocyte differentiation [ 35 ].…”

New insights into immunomodulatory properties of lactic acid bacteria fermented herbal medicines

“…NO secreted by macrophages also induces immune cell activation and promotes resistance to pathogens (Hu et al, 2021). Mo et al (2021) observed that Lacticaseibacillus paracasei HY7017 cultivated in 3% RGE-supplemented medium dramatically boosted NO production. In addition, the mRNA level of iNOS, a key enzyme in NO synthesis, and COX-2 were higher after treatment with HY7017-RGEs.…”

“…In addition, the mRNA level of iNOS, a key enzyme in NO synthesis, and COX-2 were higher after treatment with HY7017-RGEs. This process had no effect on the secretion of the anti-inflammatory cytokine IL-10, indicating that fermented red ginseng enhanced the immune response without creating excessive inflammation ( Mo et al, 2021 ).…”

Probiotic Characteristics and Safety Assessment of Lacticaseibacillus casei KGC1201 Isolated from Panax ginseng

“…Therefore, using ginseng and LAB together as materials for health functional food can provide a synergistic effect that will help to improve overall health and well-being. Moreover, as Panax ginseng has been reported to have high antibacterial activity [ 17 , 18 ], the isolation of potential probiotic strains derived from ginseng is of growing importance [ 19 , 20 ].…”