Characterization of novel Mycobacterium tuberculosis pncA gene mutations in clinical isolates from the Ukraine

Daum, L.T.; Konstantynovska, OS; Solodiankin, Oleksii; Poteiko, PI; Болотін, В. І.; Rodríguez, J.L. Martínez; Gerilovych, Anton; Chambers, JP; Fischer, Gustave-Nicolas

doi:10.1016/j.diagmicrobio.2018.10.018

Cited by 12 publications

(9 citation statements)

References 22 publications

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“…The Illumina NextSeq was the most frequently used platform for both drugs ( Figure 3B). A significant advantage of Next Generation Sequencing (NGS) over traditional Sanger sequencing is its deeper coverage [38]. In the articles reviewed here, the coverage ranged from 5X-350X, reaching the highest coverage through Illumina HighSeq4000 [39] ( Figure 3B), with higher confidence for detecting genetic variants.…”

Section: Mtb Genes Associated With Bdq and Dlm Resistancementioning

confidence: 99%

Whole Genome Sequencing for the Analysis of Drug Resistant Strains of Mycobacterium tuberculosis: A Systematic Review for Bedaquiline and Delamanid

2020

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Tuberculosis (TB) remains the deadliest Infectious disease worldwide, partially due to the increasing dissemination of multidrug and extensively drug-resistant (MDR/XDR) strains. Drug regimens containing the new anti-TB drugs bedaquiline (BDQ) and delamanid (DLM) appear as a last resort for the treatment of MDR or XDR-TB. Unfortunately, resistant cases to these drugs emerged just one year after their introduction in clinical practice. Early detection of resistant strains to BDQ and DLM is crucial to preserving the effectiveness of these drugs. Here, we present a systematic review aiming to define all available genotypic variants linked to different levels of resistance to BDQ and DLM that have been described through whole genomic sequencing (WGS) and the available drug susceptibility testing methods. During the review, we performed a thorough analysis of 18 articles. BDQ resistance was associated with genetic variants in Rv0678 and atpE, while mutations in pepQ were linked to a low-level of resistance for BDQ. For DLM, mutations in the genes ddn, fgd1, fbiA, and fbiC were found in phenotypically resistant cases, while all the mutations in fbiB were reported only in DLM-susceptible strains. Additionally, WGS analysis allowed the detection of heteroresistance to both drugs. In conclusion, we present a comprehensive panel of gene mutations linked to different levels of drug resistance to BDQ and DLM.

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Section: Mtb Genes Associated With Bdq and Dlm Resistancementioning

confidence: 99%

Whole Genome Sequencing for the Analysis of Drug Resistant Strains of Mycobacterium tuberculosis: A Systematic Review for Bedaquiline and Delamanid

2020

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“…Ukraine, with the population of approximately 45 million people, situated in the immediate neighborhood of the EU, is one of the former Soviet Union component states. Currently, Ukraine is one of the 18 high-priority countries to fight tuberculosis in the World Health Organization European Region and among the top 20 highest drug-resistant tuberculosis burden countries worldwide [17]. The results of the first national anti-tuberculosis drug resistance survey in Ukraine showed that nearly a quarter of new cases and 60% of previously treated cases had MDR-TB [18].…”

Section: Tuberculosis In the East Of The Eumentioning

confidence: 99%

“…Ukraina, jedno z tych państw o liczbie mieszkańców około 45 milionów, położona jest w bezpośrednim sąsiedztwie UE. Obecnie Ukraina należy do grupy 18 krajów Europejskiego Regionu WHO, w których zwalczanie gruźlicy ma najwyższy priorytet oraz należy do grupy 20 krajów o najczęstszym występowaniu postaci MDR na świecie [17]. Wyniki pierwszego krajowego badania przeprowadzonego na Ukrainie dotyczące występowania lekoopornej gruźlicy wykazały, że prawie jedna czwarta nowych infekcji i 60% wcześniej leczonych przypadków gruźlicy miało postać MDR [18].…”

Section: Gruźlica Na Wschodzie Unii Europejskiejunclassified

Immigrants and tuberculosis in Europe – are we prepared for outbreak?

Koper

Pajączek

Kryczka

2020

Pielegniarstwo XXI Wieku / Nursing in the 21st Century

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Introduction. International travel and immigration have led to a rise in the incidence of tuberculosis in European countries, mainly due to migration from Africa and the Middle East.Aim. In this article we point to an additional migration path - from Ukraine - and the threat of tuberculosis associated with this migration and we discuss our preparation for an increase in the incidence of this disease.Discussion. It is estimated that about 1.5-2.5 million Ukrainians work or live in Poland or the EU. This results from the implementation of some EU directives and Polish law regulations, which simplified an access of the Ukrainian citizens to the European labor market. Importantly, Ukraine is one of the 18 high-priority countries to fight tuberculosis in the WHO European Region and among the top 20 highest drug-resistant tuberculosis burden countries in the world. More importantly, there is no unitary tuberculosis screening program for migrants in the EU member states. For this reason, we have no reliable data on tuberculosis burden in any nationality group of immigrants in Europe, including Ukrainians.Conclusions. Nurses have always dealt with infectious diseases, they have always been and will be the last line of defense against the epidemic. Any epidemic, including tuberculosis. Perhaps, in the absence of an integrated tuberculosis policy in Europe, our only future are the experience, skills and dedication of nurses.

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“…We found that pncA polymorphisms were presented in 254 (81.9%) MDR and 74 (97.4%) XDR isolates ( Table 3). The proportions of pncA mutational strains were higher than those from South Africa (70% in MDR, 96% in XDR), Georgia (72% in MDR, 90% in XDR) (Allana et al, 2017), Kazakhstan (75% in MDR) (Akhmetova et al, 2015), and Ukraine (78% in MDR, 88% in XDR) (Daum et al, 2019). We also found that predicted PZA-resistant isolates based on previous literatures in Chongqing district accounts for 68.1% in MDR isolates (Table 4), which was higher than those from Beijing (57.7% in MDR) (Gu et al, 2016), Zhejiang (43.1% in MDR) (Xia et al, 2015), and Taiwan (42.9% in MDR) (Huang et al, 2003).…”

Section: Discussionmentioning

confidence: 95%

“…We found that strains with mutations in 50_51insC (Gly17), Glu174, and 521_522insT (Glu174) were all shown to be PZase negative. Previously, strains with mutations at these amino acid positions, Gly17Asp (Daum et al, 2019), Ala46Val (Cheng et al, 2000), Ser67Pro (Hameed et al, 2020), Trp68Arg , Asp80Asn (Bwalya et al, 2018), Phe81Val (Wu et al, 2019), Val93Met (Daum et al, 2019), Ser104Ile (Hameed et al, 2020), Glu127Lys (Doustdar et al, 2009), Val130Gly (Sengstake et al, 2017), Val155Ala (Daum et al, 2019), Val157Gly , Ser164Pro (Hameed et al, 2020), (27) 137_138insC (negative); (28) 491_492insC (negative); (29) 521_522insT (negative); (30) 425C > G (negative); (31) 232G > C (negative); (32) 310A > G and 388G > A (negative). Every result was shown by test using three independent colonies (mean ± SD); OD, optical density; CV, cutoff value (dash line).…”

Section: Discussionmentioning

confidence: 99%

Table_2.DOCX

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Characterization of novel Mycobacterium tuberculosis pncA gene mutations in clinical isolates from the Ukraine

Cited by 12 publications

References 22 publications

Whole Genome Sequencing for the Analysis of Drug Resistant Strains of Mycobacterium tuberculosis: A Systematic Review for Bedaquiline and Delamanid

Whole Genome Sequencing for the Analysis of Drug Resistant Strains of Mycobacterium tuberculosis: A Systematic Review for Bedaquiline and Delamanid

Immigrants and tuberculosis in Europe – are we prepared for outbreak?

Table_2.DOCX

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