Characterization of novel of temperate phages of Staphylococcus aureus isolated from bovine milk

Barasuol, Bibiana Martins; Cargnelutti, Juliana Felipetto; Sangioni, Luís Antônio; Pereira, Daniela Isabel Brayer; Varela, Ana Paula Muterle; Mayer, Fabiana Quoos; Pottker, Emanuele Serro; Gonçalves, Gabriela Flores; Cibulski, Samuel Paulo; Botton, Sônia de Ávila

doi:10.1007/s00203-022-03296-9

Cited by 2 publications

(5 citation statements)

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“…For bovine mastitis, various sample sources have been explored for the purpose of isolating phages for the various pathogens responsible for the infection ( Table 1 ). Most of the work has focused on screening raw milk samples obtained from a confirmed mastitis cattle, either directly after centrifuging and filtering of samples or via enrichment procedures to amplify and isolate phages [ 28 , 34 ]. Phages have been reported to actively bind to, lyse and amplify in milk constituents, and huge successes of phage isolation for S. aureus , S. agalactiae and S. arlettae have been recorded from this source through this method [ 7 , 28 , 29 , 34 , 40 , 41 , 46 , 49 , 63 ].…”

Section: The Case For Phage Therapy To Control Bovine Mastitismentioning

confidence: 99%

“…Most of the work has focused on screening raw milk samples obtained from a confirmed mastitis cattle, either directly after centrifuging and filtering of samples or via enrichment procedures to amplify and isolate phages [ 28 , 34 ]. Phages have been reported to actively bind to, lyse and amplify in milk constituents, and huge successes of phage isolation for S. aureus , S. agalactiae and S. arlettae have been recorded from this source through this method [ 7 , 28 , 29 , 34 , 40 , 41 , 46 , 49 , 63 ]. However, in one instance, no phage was isolated from the milk samples examined [ 48 ].…”

Section: The Case For Phage Therapy To Control Bovine Mastitismentioning

confidence: 99%

“…Host range analysis mainly focuses on phage lysis activity using spot test with the double-layer agar method (application of phage samples to confluent cultures of bacteria in a semi-solid agar medium overlayed on solid agar medium). This is to ascertain the range of relevant bacterial strains the phages can lyse with some demonstrating broad or narrower host coverage [ 28 , 36 , 37 ] ( Table 1 ). Besides phage coverage on a wide range of strains, other phages of S. aureus showed inter-species lysis, targeting S. sciuri and Rothia terrae [ 28 ] as well as E. coli [ 43 ], and K. oxytoca phage P2 lysing E. aerogenes as well [ 9 ].…”

Section: The Case For Phage Therapy To Control Bovine Mastitismentioning

confidence: 99%

“…This is to ascertain the range of relevant bacterial strains the phages can lyse with some demonstrating broad or narrower host coverage [ 28 , 36 , 37 ] ( Table 1 ). Besides phage coverage on a wide range of strains, other phages of S. aureus showed inter-species lysis, targeting S. sciuri and Rothia terrae [ 28 ] as well as E. coli [ 43 ], and K. oxytoca phage P2 lysing E. aerogenes as well [ 9 ].…”

Section: The Case For Phage Therapy To Control Bovine Mastitismentioning

confidence: 99%

“…The polymicrobial niche of bovine mastitis is also a challenge to overcome [ 5 , 6 , 16 ]. Most work conducted to date has focused on a single bacterial species in relevant infection model systems, except for example where S. aureus phages showed interspecies lysis on S. sciuri and Rothia terrae [ 28 ], and E. coli [ 43 ]. Whilst this is informative and provides useful insights into the therapeutic potential of the phages, it is still unclear how these single bacterial species targets would alleviate bovine mastitis.…”

Section: Barriers/challenges To Therapeutic Phage Application To Cont...mentioning

confidence: 99%

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Bacteriophage Therapy to Control Bovine Mastitis: A Review

Nale

McEwan

2023

Antibiotics

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Bovine mastitis is a polymicrobial disease characterised by inflammation of the udders of dairy and beef cattle. The infection has huge implications to health and welfare of animals, impacting milk and beef production and costing up to EUR 32 billion annually to the dairy industry, globally. Bacterial communities associated with the disease include representative species from Staphylococcus, Streptococcus, Enterococcus, Actinomyces, Aerococcus, Escherichia, Klebsiella and Proteus. Conventional treatment relies on antibiotics, but antimicrobial resistance, declining antibiotic innovations and biofilm production negatively impact therapeutic efficacy. Bacteriophages (phages) are viruses which effectively target and lyse bacteria with extreme specificity and can be a valuable supplement or replacement to antibiotics for bovine mastitis. In this review, we provide an overview of the etiology of bovine mastitis, the advantages of phage therapy over chemical antibiotics for the strains and research work conducted in the area in various model systems to support phage deployment in the dairy industry. We emphasise work on phage isolation procedures from samples obtained from mastitic and non-mastitic sources, characterisation and efficacy testing of single and multiple phages as standalone treatments or adjuncts to probiotics in various in vitro, ex vivo and in vivo bovine mastitis infection models. Furthermore, we highlight the areas where improvements can be made with focus on phage cocktail optimisation, formulation, and genetic engineering to improve delivery, stability, efficacy, and safety in cattle. Phage therapy is becoming more attractive in clinical medicine and agriculture and thus, could mitigate the impending catastrophe of antimicrobial resistance in the dairy sector.

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