Characterization of Novel Protein Component as Marker for Floral Origin of Jujube (<i>Ziziphus jujuba</i> Mill.) Honey

Zhang, Ying; Wang, Yuxiang; Zhao, Haoan; Zhang, Guangyan; Peng, Deju; Cao, Wei

doi:10.1021/acs.jafc.9b05190

Cited by 15 publications

(4 citation statements)

References 35 publications

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“…A previous study also reported that ZH could contain protein components, even cyclic peptides, with potential medicinal values [ 45 ]. These proteins can be used as a marker for floral origin [ 46 ]. In our case, we report a tripeptide Asp-Thr-Gly, which could be a specific marker for the floral origin of our investigated ZH.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Composition, Antioxidant, and Anticancer Activities of Sidr Honey: In Vitro and In Silico Computational Investigation

Bouali

Hamadou

Badraoui

et al. 2022

Life

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Cancer is one of the major causes of death worldwide. The repercussions of conventional therapeutic approaches present a challenge in the delivery of new effective treatments. Thus, more attention is being awarded to natural products, mainly honey. Honey could be the basis for the development of new therapies for cancer patients. The aim of this study is to assess the phytochemical profiling, antioxidant, drug-likeness properties, and anticancer activity of Ziziphus honey (ZH) derived from the Hail region of Saudi Arabia. The phytochemical profiling using high resolution-liquid chromatography mass spectrometry (HR-LCMS) revealed 10 compounds belonging to several familial classes and one tripeptide. Potential antioxidant activity was noted as assessed by DPPH (IC50 0.670 mg/mL), ABTS (IC50 3.554 mg/mL), and β-carotene (IC50 > 5 mg/mL). The ZH exerted a notable cytotoxic effect in a dose-dependent manner against three cancer cell lines: lung (A549), breast (MCF-7), and colon (HCT-116), with respective IC50 values of 5.203%, 6.02%, and 7.257%. The drug-likeness investigation unveiled that most of the identified compounds meet Lipinski’s rule. The molecular docking analysis revealed interesting antioxidant and anticancer activities for most targeted proteins and supported the in vitro findings. The Miraxanthin-III compound exhibited the most stabilized interaction. This study provides deeper insights on ZH as prominent source of bioactive compounds with potent antioxidant and anticancer effects.

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Section: Discussionmentioning

confidence: 99%

Phytochemical Composition, Antioxidant, and Anticancer Activities of Sidr Honey: In Vitro and In Silico Computational Investigation

Bouali

Hamadou

Badraoui

et al. 2022

Life

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“…Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) is commonly used as a protein-based analytical method because of a high reliability on molecules with mass range of 15–200 kDa with errors less than 10%. Obtained via SDS-PAGE analysis and identified by liquid chromatography–tandem mass spectrometry (LC–MS/MS), one Z. jujuba -derived protein (gene number Zj.jz 016003045) with a molecular weight of 19 kDa was used for the recognition of jujube honey . SDS-PAGE was also applied to classify the entomological origin of stingless bee honeys, such as Apis cerana honey and Apis mellifera honey. , In addition, chromatographic peak areas of honey from multifloral (2.96) and monofloral (1.19) honey at protein molecular weight of 220 kDa could be used to differentiate multi- and monofloral honey samples …”

Section: Identification Based On Biomacromolecular Substancesmentioning

confidence: 99%

Systematic Review of the Characteristic Markers in Honey of Various Botanical, Geographic, and Entomological Origins

Wang

Chen

et al. 2022

ACS Food Sci. Technol.

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Honey has become a highly demanded product for consumers worldwide due to its unique taste and rich nutritional and medicinal values. However, it remains challenging to identify honey’s origin as well as determine its authenticity. This review aims at summarizing the progress of research carried out in the last decade toward discriminating honeys by using characteristic markers. Studies that illustrated melissopalynology, phytochemical substances, and biomacromolecular substances to identify honey origins are discussed. Characteristic markers can be used for the identification of honey origins with main advantages of high sensitivity and accuracy. The compositions of honey are influenced by several factors including plant sources, harvest season, storage period, and maturity status. Discrimination of honey can be achieved by combining multiple parameters along with the applications of multivariate statistical analysis and machine learning.

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“…The rough estimate of protein is about 0.5%, which includes amino acids and enzymes; however, the contribution of these proteins in meeting daily requirements is marginal. The main enzymes are amylase, invertase, and oxidase [17]. Proline, which makes up 80-90% of all the amino acids in honey, is the main amino acid found in it.…”

Section: Proteinsmentioning

confidence: 99%

Honey Composition, Therapeutic Potential and Authentication through Novel Technologies: An Overview

Talha¹,

Imran²,

Ahmad³

et al. 2023

Honey - Composition and Properties

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Honey is acknowledged as a natural functional food with additional health benefits. Due to its medicinal and therapeutic properties, honey is being used in both pharmaceutical and food industries to develop products as a remedy against various types of ailments. Honey contains polyphenols, flavonoids, and other key compounds that play an important role in human health. Honey possesses anticancer and antimicrobial properties as well as contains antioxidant and anti-inflammatory substance. Some studies also highlighted the antidiabetic properties of honey. It supports the respiratory system and contributes beneficial effects to the cardiovascular system. As a functional and nutraceutical food, honey plays a significant role. Due to the modernization and digitalization in this era, the role of novel technologies for characterization and authentication of honey cannot be ignored. Hence, the main purpose of this chapter is to review the latest studies related to honey’s advantageous effects on human health and to highlight the novel technologies to detect the impurities in honey.

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Characterization of Novel Protein Component as Marker for Floral Origin of Jujube (Ziziphus jujuba Mill.) Honey

Cited by 15 publications

References 35 publications

Phytochemical Composition, Antioxidant, and Anticancer Activities of Sidr Honey: In Vitro and In Silico Computational Investigation

Phytochemical Composition, Antioxidant, and Anticancer Activities of Sidr Honey: In Vitro and In Silico Computational Investigation

Systematic Review of the Characteristic Markers in Honey of Various Botanical, Geographic, and Entomological Origins

Honey Composition, Therapeutic Potential and Authentication through Novel Technologies: An Overview

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