Characterization of novel α-galactosidase in glycohydrolase family 97 from Bacteroides thetaiotaomicron and its immobilization for industrial application

Removal of Raffinose Family Oligosaccharides from Soymilk by α-Galactosidase Immobilized on Sepabeads EC-EA and Sepabeads EC-HA

“…Therefore, they may be a good candidate for food processes. The immobilized α-galactosidases showed a better preference for stachyose than raffinose as reported in similar studies. , However, Shin et al found an opposite result for this subject with 98.9% degradation degree for RFO degradation …”

“…Enzymatic degradation of RFOs with α-galactosidases is a very attractive and dependable method that is suggested by several food researchers to improve the nutritional value of soy milk and soy products. This process decreases the gastric stress and can promote their consumption by humans and monogastric animals. ,,,, The ability of free and immobilized α-galactosidases to hydrolyze RFOs in soy milk was investigated, and the products were analyzed by HPLC. The total oligosaccharide content of soymilk was determined as 54 mg/mL.…”

“…Wang et al also used GalC for this aim, and they determined that the enzyme was very effective for the removal of raffinose and stachyose . Shin et al used an immobilized α-galactosidase from Bacterioides thetaiotaomicron and the enzyme eliminated 98.9% of RFOs . The crosslinked α-galactosidase aggregates from maize also efficiently hydrolyzed 85% of raffinose and 96% of stachyose …”

“…They are really valuable enzymes in the food industry like in processing of soy, sugar beet, and guar gum, animal feed formulation, and synthesis of prebiotic galactooligosaccharides. Many researchers have especially focused on the use of free and immobilized α-galactosidases in these food applications. − RFOs, mainly raffinose and stachyose, are complex α- d -galactooligosaccharides that are also identified as an antinutritional factor. They cannot be digested by humans and monogastric animals because of α-galactosidase deficiency, and also, they can therefore cause flatulence and other gastrointestinal disorders.…”

“…Enzymatic treatment with α-galactosidases has been proven to be the most effective and promising method for RFO degradation and removal. Therefore, to find and to develop an enzyme with high stability and hydrolysis ability toward RFOs are necessary to meet the industrial demands. ,,,, …”