Characterization of NT-proBNP in Human Urine

Abstract

BACKGROUND: Urine amino-terminal probrain natriuretic peptide (NT-proBNP) concentrations may exclude the presence of heart failure and provide insight into renal clearance mechanisms for human NTproBNP. We characterized the molecular forms of urine NT-proBNP detected by immunoassay.

Elevated Levels of N-Terminal Pro-Brain Natriuretic Peptide in Patients with Chronic Dyspnea and Moderate Renal Dysfunction: Decreased Clearance or Increased Cardiac Stress

“…Rather, our data are consistent with the hypothesis that tubular secretion or metabolism is the major renal clearance mechanism for circulating NT-proBNP. There is some evidence in the literature that renal NT-proBNP clearance by tubular mechanisms is upregulated rather than reduced with decreasing eGFR [12]. Our data also support the notion that the disproportionate increase in the ln(NT-proBNP) values seen in the group of patients with heart disease and at least moderate renal impairment is the result of increased cardiac secretion.…”

Measurement of Urinary Amino-Terminal Pro-Brain Natriuretic Peptide in Childhood Lower Respiratory Tract Infections: An Indicator of Clinical Severity?

“…Studies have assessed the diagnostic and prognostic value of NT-ProBNP in urine as a simple and non-invasive test (11,28). Although slightly lower values than plasma are detected, urine NT-ProBNP was shown to have a diagnostic accuracy to that of plasma NT-ProBNP for the diagnosis of heart failure in adults (29).…”

“…Research assessing plasma peptide levels in arrytmia, febrile convulsion, epilepsy, syncope, sepsis, and more have further been conducted (8)(9)(10). NT-ProBNP is also found in the urine as small peptides undergo renal filtration (11). Studies measuring urinary NT-ProBNP levels in children are generally limited to the prediction and follow-up of hemodynamically significant patent ductus arteriosus (hs-PDA) in preterm infants (12,13).…”

Diagnostic Values of Plasma, Fresh and Frozen Urine NT-proBNP in Heart Failure Patients

“…The positive correlation between plasma and urine (fresh and frozen) NT-proBNP concentrations in CHF patients is demonstrated in previous studies with correlation coefficients ranging between r=0.53 and r=0.78. 15,17,18,21 The report by Jungbauer et al 22 about fresh urine NT-proBNP showed a correlation coefficient 0.79. These were higher than evaluated correlations for fresh and frozen urine NT-proBNP in our study.…”

“…15-21 However, studies of urinary NT-proBNP are limited, particularly studies using fresh, unfrozen samples. 22 This evaluation could be useful in certain circumstances.…”