Characterization of nuclear microsatellite loci in the calcareous fen specialist <i>Scorpidium cossonii</i> (Calliergonaceae)

Kophimai, Yuppayao; Cornejo, Carolina; Peintinger, Markus; Bergamini, Ariel; Scheidegger, Christoph

doi:10.3732/ajb.1100144

Cited by 3 publications

(3 citation statements)

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“…Scorpidium scorpioides is a further possibility. Twelve microsatellites were amplified in S. scorpioides (Kophimai et al, 2011) and morphologically it is even closer to S. revolvens than to S. cossonii, as was shown by Hedenä s and Eldenä s (2008). Hamatocaulis vernicosus (Mitt.)…”

Section: Ploidy Levelmentioning

confidence: 80%

“…Very likely, S. cossonii is one of the parents as it is morphologically very similar to S. revolvens (Hedenä s, 1989), all 14 primers of the microsatellites developed for S. cossonii successfully amplified in S. revolvens (Kophimai et al, 2011), and 13 microsatellite loci contain the same repeat type in the two species. Moreover, some alleles are shared between the two species while one allele had a high frequency in both species.…”

Section: Ploidy Levelmentioning

confidence: 97%

“…Microsatellite sequences and protocols for DNA extraction, PCR amplification and allele sizing are described in Kophimai et al (2011). For S. revolvens, Figure 1 Map of the study populations in the eastern Swiss Alps.…”

Section: Microsatellite Analysismentioning

confidence: 99%

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Ploidy level, genetic diversity, and differentiation in two closely related mosses,Scorpidium cossoniiandS. revolvens(Calliergonaceae)

Kophimai¹,

Peintinger²,

Werth³

et al. 2013

Journal of Bryology

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As ploidy level and mating system can affect genetic diversity and differentiation, we conducted population genetic analyses of two closely related mosses, Scorpidium cossonii (Schimp.) Hedenä s, and S. revolvens (Sw. ex Anonymo) Rubers which differ in ploidy level and sexual system. We collected 315 specimens in total from five populations of S. cossonii and four populations of S. revolvens in the Swiss Alps. Ploidy level, genetic diversity within populations, and genetic differentiation between populations and species were estimated using nine microsatellite markers. In each S. cossonii sample, each locus bore only one allele, while in S. revolvens, seven out of the nine loci were fixed or nearly fixed for two alleles per locus per individual. These findings are consistent with a gametophytic haploid S. cossonii and allodiploid S. revolvens. The haploid and dioicous S. cossonii was genetically more diverse than the (allo)diploid and monoicous S. revolvens. Differences in genetic diversity between the two species may be explained by different mating systems, different population sizes, and different population histories. Genetic differentiation among populations of S. cossonii was higher than among those of S. revolvens. The low genetic differentiation among populations of the monoicous species was not unexpected, since monoicous species frequently produce sporophytes, long-distance spore dispersal is more likely and leads to low differentiation.

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Section: Ploidy Levelmentioning

confidence: 80%

Section: Ploidy Levelmentioning

confidence: 97%