Characterization of Oxytocin, Vasopressin, and Neurophysin from the Bovine Corpus Luteum*

Wathes, D C; Swann, R. W.; Birkett, Sonia D.; Porter, D. G.; Pickering, B. T.

doi:10.1210/endo-113-2-693

Cited by 133 publications

(59 citation statements)

References 17 publications

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“…Measurements were made on the same extracts used for the oxytocin assay, using the specific radioimmunoassay described previously by Wathes et al (1983), using an antiserum (84/1) raised and charac¬ terized in the Department of Anatomy. This antiserum cross-reacts <6% with lysine vasopressin and arginine vaso¬ tocin, and <003% with oxytocin, mesotocin, and the neurophysins.…”

Section: Introductionmentioning

confidence: 99%

Pulsatile release of oxytocin into the circulation of the ewe during oestrus, mating and the early luteal phase

Gilbert¹,

Jenkins²,

Wathes³

1991

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ranged from 1\m=.\5\ m=+-\0\m=.\53 to 6\m=.\8\ m=+-\5\m=.\25 pg/ml in different animals. Ewes which were both in oestrus and exposed to the ram showed a pulsatile oxytocin release pattern consisting of low baseline concentrations with short-duration pulses superimposed (duration 1\p=n-\4 min; amplitude 2\ m=. \ 5\ p=n-\ 31\ m=. \ 7 pg/ml; frequency 3\m=.\18/h). Coitus was not temporally associated with pulsatile release. However, the importance of the presence of the ram was indicated by total separation of 2 oestrous ewes from the ram until after experimentation. In these animals only 1 pulse of oxytocin was detected in 2\m=.\7h of sampling.It is concluded that, although mean oxytocin concentrations at oestrus were low, short duration pulses were released into the plasma at this time. This effect may be dependent on the presence of a ram.

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Section: Introductionmentioning

confidence: 99%

Pulsatile release of oxytocin into the circulation of the ewe during oestrus, mating and the early luteal phase

Gilbert¹,

Jenkins²,

Wathes³

1991

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“…These results suggest that oxytocin may be derived from the corpus luteum (CL) and be involved in the process ofluteolysis. Oxytocin has been detected in the CL of sheep , cows (Wathes et al, 1983) sheep and women by use of high performance liquid chromatography, milk ejection and rat uterus strip bioassays and radioimmunoassays (RIA) . However the RIA employed are open to criticism on the grounds that immunological activity can be separated from biological activity, and the RIA may measure degraded and biologically inactive fragments of OT (Kumaresan et al, 1969).…”

Section: Introductionmentioning

confidence: 99%

Evidence for the presence of oxytocin in the corpus luteum of the goat

Homeida

1986

British J Pharmacology

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1 Purified acetic acid extracts of corpora lutea (CL) of non-pregnant goats were found to contain substantial amounts of oxytocin (OT) as measured by radioimmunoassay.2 OT standard and the CL extracts released prostaglandin F2, (PGF2,) from rat isolated uteri in a quantitatively similar manner. 3 Treatment of both OT standard and CL extract with sodium thioglycolate, oxytocin antiserum or oxytocin antagonist abolished this biological activity. 4 Acid extracts ofCL of pregnant goat were found to contain approximately 2% of levels during the cycle by day 21 after fertile mating and this had a reduced ability to release PGF2. from rat uterus.5 It is concluded that both the immunoreactivity and the biological activity ofthe CL extracts are due to the presence of an oxytocin-like substance and that tissue levels of oxytocin are low in pregnant compared to non-pregnant goats.

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“…Il est tentant -pour ne pas dire logique -de lier le taux de l'ocytocine à ces deux stéroïdes, ce que firent D.C. WATHES et al [34].…”

Section: Ocytocine Et Corps Jaune Chez Les Ruminantsunclassified

L’ocytocine : données nouvelles et perspectives

Constantin¹

1992

bavf

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L’extrait post-hypophysaire a été introduit dans la Pharmacopée au début du siècle, l’indication principale était le traitement des insuffisances de la contraction utérine. Peu à peu, on a compris qu’il y avait deux hormones peptidiques dans l’extrait : l’ocytocine (1953) et la vasopressine (1964). La notion de neuro-sécrétion est apparue en même temps (1956). Des neurophysines spécifiques sont couplées à ces deux hormones. On a également analysé les hormones hypothalamiques dans toute la série animale, et constaté qu’elles étaient de bons marqueurs des étapes de la phylogénèse. L’hypothalamus n’a pas l’exclusivité de la synthèse de l’ocytocine, des cellules lutéales (granulosa) en font de même, mais en quantité beaucoup plus faibles. Enfin, des recherches sur le rat ont permis de mettre en évidence une action négative sur la mémorisation et une action positive sur le comportement maternel.

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Characterization of Oxytocin, Vasopressin, and Neurophysin from the Bovine Corpus Luteum*

Cited by 133 publications

References 17 publications

Pulsatile release of oxytocin into the circulation of the ewe during oestrus, mating and the early luteal phase

Pulsatile release of oxytocin into the circulation of the ewe during oestrus, mating and the early luteal phase

Evidence for the presence of oxytocin in the corpus luteum of the goat

L’ocytocine : données nouvelles et perspectives

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