Characterization of Papaya ringspot virus isolates infecting transgenic papaya ‘Huanong No.1’ in South China

Wu, Zilin; Mo, Cuiping; Zhang, Shuguang; Li, Huaping

doi:10.1038/s41598-018-26596-x

Cited by 22 publications

(20 citation statements)

References 63 publications

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“…▲ is for Hainan, and ■ is for Guangdong and the occurrence tendency increases gradually with time (Zhao et al, 2015). To explain the loss of transgenic papaya resistance against PRSV in South China, Wu et al (2018) No viral symptom was observed, and PLDMV was not detected on squash leaves; however, transgenic papaya plants showed obvious infection symptoms, and PLDMV was detected ( Figure 2). These results confirmed, on the one hand, that the isolates we obtained from the samples collecting from Guangdong and Hainan provinces are the agents of the disease and, on the other hand, that the infection characteristics revealed that the isolates from Hainan and Guangdong provinces are similar to the Taiwan China isolates rather than Japan isolates.…”

Section: Discussionmentioning

confidence: 99%

“…To explain the loss of transgenic papaya resistance against PRSV in South China, Wu et al. (2018) investigated the virus occurrence in “Huanong No.1” papaya plantations in Guangdong and Hainan in 2012–2016. They identified a genetic variation of PRSV and revealed that the formation of new emergent lineage of PRSV may be one of the reasons for the loss of resistance to genetically modified papaya in South China.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, a short polypeptide (PIPO) is expressed within the P3 cistron by frame shifting (Chung et al, 2008). Among the 11 encoded functional proteins, the coat protein (CP) sequence has been frequently used in strain identification, species classification, and phylogenetic analysis of potyviruses (Chaves‐Bedoya & Ortiz‐Rojas, 2015; Cuevas et al, 2012; Gao et al, 2016; Gibbs & Ohshima, 2010; Martinez, 2014; Wu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity analysis of papaya leaf distortion mosaic virus isolates infecting transgenic papaya “Huanong No. 1” in South China

Xie

et al. 2020

Ecology and Evolution

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The commercialized genetically modified papaya “Huanong No. 1” has been utilized to successfully control the destructive virus‐papaya ringspot virus (PRSV) in South China since 2006. However, another new emerging virus, papaya leaf distortion mosaic virus (PLDMV), was found in some PRSV‐resistant transgenic plants in Guangdong and Hainan provinces of South China through a field investigation from 2012 to 2019. The survey results showed that “Huanong No. 1” papaya plants are susceptible to PLDMV, and the disease prevalence in Hainan Province is generally higher than that in Guangdong Province. Twenty representative isolates were selected to inoculate “Huanong No. 1,” and all of the inoculated plants showed obvious disease symptoms similar to those in the field, indicating that PLDMV is a new threat to widely cultivated transgenic papaya in South China. Phylogenetic analysis of 111 PLDMV isolates in Guangdong and Hainan based on the coat protein nucleotide sequences showed that PLDMV isolates can be divided into two groups. The Japan and Taiwan China isolates belong to group I, whereas the Guangdong and Hainan isolates belong to group II and can be further divided into two subgroups. The Guangdong and Hainan isolates are far different from the Japan and Taiwan China isolates and belong to a new lineage. Further analysis showed that the Guangdong and Hainan isolates had a high degree of genetic differentiation, and no recombination was found. These isolates deviated from neutral evolution and experienced population expansion events in the past, which might still be unstable. The results of this study provide a theoretical basis for clarifying the evolutionary mechanism and population genetics of the virus and for preventing and controlling the viral disease.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic diversity analysis of papaya leaf distortion mosaic virus isolates infecting transgenic papaya “Huanong No. 1” in South China

Xie

et al. 2020

Ecology and Evolution

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“…Phylogenetic analysis revealed the presence of a new virus lineage in Hainan and Guangdong papaya plantations, which may pose a threat to Huanong No. 1 papaya cultivation 25 .…”

Section: Papaya Resistant To Papaya Ringspot Virusmentioning

confidence: 99%

Transgenic and genome-edited fruits: background, constraints, benefits, and commercial opportunities

et al. 2021

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Breeding has been used successfully for many years in the fruit industry, giving rise to most of today’s commercial fruit cultivars. More recently, new molecular breeding techniques have addressed some of the constraints of conventional breeding. However, the development and commercial introduction of such novel fruits has been slow and limited with only five genetically engineered fruits currently produced as commercial varieties—virus-resistant papaya and squash were commercialized 25 years ago, whereas insect-resistant eggplant, non-browning apple, and pink-fleshed pineapple have been approved for commercialization within the last 6 years and production continues to increase every year. Advances in molecular genetics, particularly the new wave of genome editing technologies, provide opportunities to develop new fruit cultivars more rapidly. Our review, emphasizes the socioeconomic impact of current commercial fruit cultivars developed by genetic engineering and the potential impact of genome editing on the development of improved cultivars at an accelerated rate.

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“…PRSV-P emergence reported in 2009 onwards12 . However, we have studied constantly the emerging PRSV-P in Pakistan to develop management strategies against its widespread and found it stably conserved and devastating in the country.The mutation prone RNA viruses mark recombination and genetic differentiation which can lead to evolution as biodiversity20 . The study on genetic differentiation and gene flow between PRSV populations from diverse localities is of critical importance keeping in view this newly emerged atypical PRSV cluster from India, Bangladesh and Pakistan.…”

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confidence: 99%

Genetic variability and evolutionary dynamics of atypical Papaya ringspot virus infecting Papaya.

Saleem

Ali

Yeh

et al. 2020

Preprint

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The mutation prone RNA genome of Papaya ringspot virus could be a driving force behind its geographical spread and dissemination. Here we present the molecular investigations on atypical PRSV–P strain identified from Pakistan with genome sequence phylogenetic and recombination analysis. The PRSV-P, Pakistan outbreak strain showing a geographic spread across India and Bangladesh in phylogenetic lineage. In major recombination events, it has acquired genome variation in amino terminal of PRSV coat protein gene, whereas the gene for helper component-proteinase (HC–Pro), a nonstructural coding region of multi-domain provenance, also evolves at nucleotide and amino acid levels. The phylogenetic analysis of another highly variable P1 region showed evolutionary dynamics with respect to other geographical strains, particularly the Indian isolate from North East region (Meghalaya). PRSV–PK holds high levels of genetic divergence in comparison to American, Australian and Asian isolates. The genetic and phylogeographic analyses indicate that a spatial recombination has occurred from first PRSV, however temporal evolution is within the region of occurrence. The genetic variations and evolutionary dynamics of this virus may challenge the resistance developed in papaya against PRSV and give rise to virus lineage because of its atypical emergence where geographic spread is already occurring.

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Characterization of Papaya ringspot virus isolates infecting transgenic papaya ‘Huanong No.1’ in South China

Cited by 22 publications

References 63 publications

Genetic diversity analysis of papaya leaf distortion mosaic virus isolates infecting transgenic papaya “Huanong No. 1” in South China

Genetic diversity analysis of papaya leaf distortion mosaic virus isolates infecting transgenic papaya “Huanong No. 1” in South China

Transgenic and genome-edited fruits: background, constraints, benefits, and commercial opportunities

Genetic variability and evolutionary dynamics of atypical Papaya ringspot virus infecting Papaya.

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