Characterization of Partial Coding Region Fibroin Gene on Wild Silkmoth Cricula trifenestrata Helfer (Lepidoptera: Saturniidae)

Abstract

The study was conducted to characterize coding region of wild silkmoth C. trifenestrata partial fi broin gene, and detect these gene potential as molecular marker. A total of six larvae C. trifenestrata were collected from Bogor, Purwakarta and Bantul Regency. Genomic DNA was extracted from silk gland individual larvae, then amplifi ed by PCR method and sequenced. DNA sequenced result was 986 nucleotide partial fi broin gene of C. trifenestrata, which are comprising complete coding region of fi rst exon (42 nucleotide), an intron (113 nucleotide), and partial of second was exon (831 nucleotide). Only coding region was characterized. Results showed that fi rst exon very conserved in C. trifenestrata. These gene consisted of 31%, thymine, 28% guanine, 21% cytosine, and 19% adenine. Cytosine and thymine (sites of 25 th and 35 th respectively) were marker for C. trifenestrata species. The fi rst exon encoding 14 amino acids. Valine amino acid (12 th site) was marker to the species C. trifenestrata. The partial second exon consisted of guanine (32.7%), alanine (26.5%), thymine (21%) and cytosine (19.7%). These region encoded 277 amino acids, which were dominated by the alanine (27.8%) and glycine (21.66%). Alanine formed polyalanine sequence with diff erent motifs namely: AAAAAAASS, AAAAAAAAAAAGSSG, AAAAAAAAAAAAGSGTGFGGYDS, AAAAAAAAAAGS SGRGGYDGVDGGYGSGSS, and AAAAAAAAAAAAGSSGRGLGGYDGWVDDGYGSGSGS.

Gene expression analysis in the larval silk gland of the eri silkworm Samia ricini

“…h‐fibroin is a large and highly repetitive gene whose product serves as a major component of silk fiber. Its nonrepetitive N‐terminal or C‐terminal sequence has been identified in several lepidopteran families such as Saturniidae, Bombycidae, Pyralidae, Yponomeutidae, and Hepialidae (Sezutsu & Yukuhiro, ; Fedič et al ., ; Yonemura & Sehnal, ; Sezutsu et al ., , b; Collin et al ., ; Sezutsu et al ., ; Suriana et al ., ). We found that the products of contig 27 (accession number LC001862) and contig 61 (LC001861) were homologous to the C‐termini and N‐termini of Bombyx H‐fibroin and other Saturniidae fibroins (Fig.…”

An Insight into Genomics of Mulberry Silkworm

“…Gene mapping of silkworm started in late 19's but the very first genetic map of silkworm was constructed by [14]. Gene mapping has led to the identification of 1018 genetic markers with an approximate interval of 500 kb, and it was carried out in twenty-seven autosomes and one Z chromosome [8]. By using backcross population, linkage map was constructed with 85 STS markers, 80 SSRM markers and 16 single nucleotide polymorphisms.…”

“…Easy handling of silkworm makes it the second most successful organism in laboratories apart from drosophila. Since Lepidoptera contains some agricultural pests, so the genetic study of silkworm is helpful in their control [7,8].…”

Optimization of Silkworm Sericin Extraction Attacus atlas and Samia cynthia ricini Using Response Surface Methodology

“…The protein extraction was previously conducted by , adding 2 g/L (0.05 N) NaOH, 2 cc/L teepol, and 2 g/L neutral soap to boiling A. atlas cocoons at 80°C for 2 hours will produce a better character of the silk fiber in terms of fiber length and weight. The strong base NaOH 0.1 N is a solvent for degumming which produces the best fibroin of C. trifenestrata (Suriana 2011). Furthermore, the extraction with a combination of temperature, time, and solvent (NaOH) at a certain concentration is important to optimize sericin yields.…”