Characterization of pathogenic and nonpathogenic strains of Xanthomonas axonopodis pv. manihotis by PCR-based DNA fingerprinting techniques

Gonzalez, C

doi:10.1016/s0378-1097(02)00913-8

Cited by 7 publications

(14 citation statements)

References 21 publications

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“…Although some non-pathogenic Xam strains have been characterized genetically and phenotypically, little is known about the epidemiological importance of such strains in CBB disease (Restrepo and Verdier, 1997;Gonzalez et al, 2002). As was recently observed in Colombia and in Africa, changes in the pathogen's population structure may lead to the overcoming of the deployed resistance (Restrepo et al, 2000c;Wydra and Verdier, 2002).…”

Section: Introductionmentioning

confidence: 99%

Recent progress in the characterization of molecular determinants in the Xanthomonas axonopodis pv. manihotis–cassava interaction

et al. 2004

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Cassava bacterial blight, caused by Xanthomonas axonopodis pv. manihotis (Xam), is a widespread disease that affects cassava (Manihot esculenta Crantz). Studies on the pathogen population structure, pathogen diagnosis, identification and expression of plant genes involved in resistance have been carried out. Different molecular techniques were developed to assess the genetic diversity among the Xampopulations. Characterization of Xam population dynamics over time had enable us to determine the different factors that are associated with resistance breakdown and those that influence the genetic structure or virulence phenotypes of the pathogen's population. Methods for detecting the pathogen in vegetative planting materials and true seeds were developed and contributed to reduce the impact of the disease. To better understand the genetics of resistance a quantitative trait loci (QTLs) approach was developed. Using a PCR-based strategy with degenerate primers we isolated two resistance gene candidates in cassava. We also characterized a region of a chromosome rich in R-gene like sequence. In this review we also report the main results obtained by transcript profiling methodologies, cDNA-AFLP and ESTs developed by the authors to characterize the genes involved in disease resistance. All together these techniques allowed the identification of molecular markers either associated to CBB resistance or that may represent putative genes involved in disease resistance. This article reviews current knowledge on the molecular cassava-Xam interactions.

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Section: Introductionmentioning

confidence: 99%

Recent progress in the characterization of molecular determinants in the Xanthomonas axonopodis pv. manihotis–cassava interaction

et al. 2004

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“…Xanthomonas strains living in close association with plants but causing no apparent disease symptoms on their host have been reported (2,3). On the basis of amplified fragment length polymorphism (AFLP) analysis, Gonzalez et al (2) showed that nonpathogenic Xanthomonas strains colonizing cassava were clearly distinguished from Xanthomonas axonopodis pv. manihotis strains that cause cassava bacterial blight.…”

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confidence: 99%

Phylogenetic and Variable-Number Tandem-Repeat Analyses Identify Nonpathogenic Xanthomonas arboricola Lineages Lacking the Canonical Type III Secretion System

Essakhi

Cesbron

Saux

et al. 2015

Appl Environ Microbiol

118

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bXanthomonas arboricola is conventionally known as a taxon of plant-pathogenic bacteria that includes seven pathovars. This study showed that X. arboricola also encompasses nonpathogenic bacteria that cause no apparent disease symptoms on their hosts. The aim of this study was to assess the X. arboricola population structure associated with walnut, including nonpathogenic strains, in order to gain a better understanding of the role of nonpathogenic xanthomonads in walnut microbiota. A multilocus sequence analysis (MLSA) was performed on a collection of 100 X. arboricola strains, including 27 nonpathogenic strains isolated from walnut. Nonpathogenic strains grouped outside clusters defined by pathovars and formed separate genetic lineages. A multilocus variable-number tandem-repeat analysis (MLVA) conducted on a collection of X. arboricola strains isolated from walnut showed that nonpathogenic strains clustered separately from clonal complexes containing Xanthomonas arboricola pv. juglandis strains. Some nonpathogenic strains of X. arboricola did not contain the canonical type III secretion system (T3SS) and harbored only one to three type III effector (T3E) genes. In the nonpathogenic strains CFBP 7640 and CFBP 7653, neither T3SS genes nor any of the analyzed T3E genes were detected. This finding raises a question about the origin of nonpathogenic strains and the evolution of plant pathogenicity in X. arboricola. T3E genes that were not detected in any nonpathogenic isolates studied represent excellent candidates to be those responsible for pathogenicity in X. arboricola. E ubacteria constitute a major component of the commensal microbiota, and the nature of their interaction with plants is still unknown (1). Xanthomonas strains living in close association with plants but causing no apparent disease symptoms on their host have been reported (2, 3). On the basis of amplified fragment length polymorphism (AFLP) analysis, Gonzalez et al. (2) showed that nonpathogenic Xanthomonas strains colonizing cassava were clearly distinguished from Xanthomonas axonopodis pv. manihotis strains that cause cassava bacterial blight. Although some of these nonpathogenic strains have been characterized genetically and phenotypically, little is known about their epidemiological or ecological importance.In previous studies, the genetic diversity and population structure of Xanthomonas have been investigated using DNA-DNA hybridization (4, 5), repetitive-sequence PCR (rep-PCR) (4-7), AFLP (4, 8), and fluorescent AFLP (9, 10). As an alternative to these methods, the comparative sequence analysis of protein-encoding genes has also been widely explored. For example, Parkinson et al. (11) used the gyrB gene, which encodes the subunit B protein of DNA gyrase, for establishing a phylogenetic relationship among 203 Xanthomonas pathotype strains. Young et al. (12, 13) used a multilocus sequence analysis (MLSA) based on four genes (dnaK, encoding the chaperone protein; fyuA, encoding one tonB-dependent transporter; gyrB and rpoD, encoding the R...

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“…AFLP has proven to be useful for identifying bacterial strains at subgeneric level [5,10,11,14,15,17,18,20]. Although the results of AFLP fingerprinting are specific and show high resolution to differentiate bacterial strains at the subgeneric level, it is very clear that the efficacy of this method not only depends on the choice of a suitable restriction enzyme that cleaves the most stable site in the bacterial genome but also depends on application of suitable primer to amplify the unique fragment of bacterial genome which can differentiate one strain from the others.…”

Section: Resultsmentioning

confidence: 99%

“…Rademaker et al [17] compared AFLP, rep-PCR and DNA-DNA homology for the strains of genus Xanthomonas and observed high correlation, suggesting that genomic fingerprinting techniques truly reveal genotypic and phylogenetic relationships of organisms. Gonzalez et al [5] using AFLP distinguished pathogenic and nonpathogenic strains of X. axonopodis pv. manihotis from each other and also from other Xanthomonas strains.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Pathogenicity of Xanthomonas axonopodis Strains Inducing Citrus Canker Disease in Iran and South Korea

Khodakaramian

Swings²

2011

Indian J Microbiol

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Characterization of pathogenic and nonpathogenic strains of Xanthomonas axonopodis pv. manihotis by PCR-based DNA fingerprinting techniques

Cited by 7 publications

References 21 publications

Recent progress in the characterization of molecular determinants in the Xanthomonas axonopodis pv. manihotis–cassava interaction

Recent progress in the characterization of molecular determinants in the Xanthomonas axonopodis pv. manihotis–cassava interaction

Phylogenetic and Variable-Number Tandem-Repeat Analyses Identify Nonpathogenic Xanthomonas arboricola Lineages Lacking the Canonical Type III Secretion System

Genetic Diversity and Pathogenicity of Xanthomonas axonopodis Strains Inducing Citrus Canker Disease in Iran and South Korea

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