Characterization of PEI-coated superparamagnetic iron oxide nanoparticles for transfection: Size distribution, colloidal properties and DNA interaction

Steitz, Benedikt; Hofmann, Heinrich; Kamau, Sarah W.; Hassa, Paul O.; Hottiger, Michael O.; Rechenberg, Brigitte von; Hofmann-Amtenbrink, Margarethe; Petri‐Fink, Alke

doi:10.1016/j.jmmm.2006.10.1194

Cited by 92 publications

(59 citation statements)

References 23 publications

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“…Their positive zeta potential and aggregate size are both functions of the weight ratio of PEI to Fe 3 O 4 . Higher ratios lead to more positive zeta potentials and smaller aggregates of MP-PEI, which is consistent with the results reported by Petri-Fink and co-workers [11]. By changing the weight ratio, we could control the zeta potential in the range +10 to +50 mV, and the aggregate size in the range 200 to 800 nm.…”

Section: Resultssupporting

confidence: 91%

“…Besides bioconjugation, a much more simple method involves using positively charged magnetic particles to couple with nuclear acids via electrostatic interactions [10,11]. One of the most widely used positively charged modification agents is polyethyleneimine (PEI) which is capable of forming complexes with DNA and condensing them into compact nanoparticles [12,13].…”

Section: Nano Researchmentioning

confidence: 99%

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Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection

et al. 2009

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Using magnetic nanoparticles to enhance gene transfection, a recently developed technique termed magnetofection, has been shown to be a powerful technology in gene delivery. The most widely used magnetic nanoparticles in this area are those coated with polyethyleneimine, which is a well known nonviral transfection agent. In this article, we report methods to control the aggregate size of polyethyleneiminecoated magnetite particles. These particles were then used to enhance transfection of green fl uorescent protein (GFP) into NIH 3T3 cells in vitro. We find that the aggregate size of the particles has a great effect on their performance in magnetofection, with less aggregated magnetic particles being more effective in enhancing the gene transfection.

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Section: Resultssupporting

confidence: 91%

Section: Nano Researchmentioning

confidence: 99%

Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection

et al. 2009

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“…SPIONs coated with polyethylenimine (PEI), a cationic polymer, interact electrostatically with negatively charged DNA, demonstrating their applicability as transfection agents. 42 Similarly, dextran-coated SPIONs functionalized with negatively charged functional groups can couple with peptide oligomers via electrostatic interactions. 43 Because of hydrophobic interactions, lipophilic drugs can easily be attached to SPIONs covered with hydrophobic polymers, from where the drug can be released when the coating degrades.…”

Section: Drug Loadingmentioning

confidence: 99%

“…DNA-PEI-SPION complexes also show reduced toxicity compared with PEI-DNA complexes. 42 expression of plasminogen activator inhibitor-1 involved in various vascular disorders, including vascular inflammation and atherosclerosis. 112 Hwang et al immobilized an adenoassociated virus delivery system on heparin-coated SPIONs.…”

Section: Gene Therapymentioning

confidence: 99%

Superparamagnetic iron oxide nanoparticles: magnetic nanoplatforms as drug carriers

Wahajuddin¹,

Arora²

2012

IJN

915

574

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“…Interestingly, selected small functional iron oxide NPs (in the size range of 5-30 nm) are now commercially available and FDA-approved [6] for clinical use (mainly MRI). In this context, numerous in vitro toxicity studies concerning different iron oxide particles functionalized by a great variety of polymers [7,8] such as polyethylene imine (PEI) [9] PVA (polyvinyl alcohol), PLL (poly-L-lysine), PDMA (poly N,Ndimethylacrylamide) and PEGs (polyethylene glycols) [10] have recently been reviewed, showing the major importance of correctly designed NPs surface engineering.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound-Mediated Surface Engineering of Theranostic Magnetic Nanoparticles: An Effective One-Pot Functionalization Process Using Mixed Polymers for siRNA Delivery

Israel¹,

Lellouche²

2016

J Nanomed Nanotechnol

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Nano-sized materials have been studied for diverse clinical applications, partly because their size-dependent physical properties and nanometer-scale dimensions have important roles in biological systems. Synergistic combinations of differently nanostructured materials, such as polymer-coated magnetic nanoparticles (NPs), strongly promoted various multifunctional nano-medical platforms for simultaneous diagnosis and therapy in the rapidly emerging area of theranostics.In this context, magnetically responsive Ce cation-doped maghemite (γ-Fe 2 O 3 ) NPs form a useful NPs basis towards a new type of polycationic polymer/organic species-grafted maghemite NPs for both drug delivery and imaging. The versatility of the Ce cation-doped maghemite NPs fabrication process mediated by high-power ultrasound (US) enables the development of a new one-step time-saving US-driven variant fabrication of corresponding polymer/organic species-grafted NPs. Thus, two types of organic polycationic species, a branched 25 kDa polyethylene imine (b-PEI 25 ) polymer and a generation 2 (G2) PAMAM (poly(amidoamine)) dendrimer biopolymer, were simultaneously used during this US-mediated NPs system fabrication to effectively deliver optimized small interfering RNA (siRNA) applications as a proof of concept. This unique one-step fabrication protocol affords a positively charged magnetic core grafted with mixed organic species nanocomposite particles that enables both gene silencing therapy and magnetic resonance imaging.

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Characterization of PEI-coated superparamagnetic iron oxide nanoparticles for transfection: Size distribution, colloidal properties and DNA interaction

Cited by 92 publications

References 23 publications

Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection

Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection

Superparamagnetic iron oxide nanoparticles: magnetic nanoplatforms as drug carriers

Ultrasound-Mediated Surface Engineering of Theranostic Magnetic Nanoparticles: An Effective One-Pot Functionalization Process Using Mixed Polymers for siRNA Delivery

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