Characterization of Penicillium oxalicum SL2 isolated from indoor air and its application to the removal of hexavalent chromium

Long, Bibo; Ye, Binhui; Liu, Qinglin; Shu, Zhang; Ye, Jien; Zou, Lina; Shi, Jiyan

doi:10.1371/journal.pone.0191484

Cited by 33 publications

(14 citation statements)

References 61 publications

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“…Cr(VI) is 100 times toxic and 1000 times more mutagenic compared to Cr(III) (Chang et al 2019). Reducing Cr(VI) to Cr(III) in Cr(VI) removal is therefore an effective method for signi cantly reducing the toxic effect of Cr(VI) (Long et al 2018;Pan et al 2014). Cr(VI) is recognized as one of the priority pollutants by the United States Environmental Protection Agency (USEPA) (USEPA 2014).…”

Section: Introductionmentioning

confidence: 99%

Bioreduction and bioremoval of hexavalent chromium by genetically engineered strains (Escherichia coli MT2A and Escherichia coli MT3)

Akkurt

Oğuz

Uçkun

2022

World J Microbiol Biotechnol

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Studies on the removal of toxic metals from water by genetic engineering applications are becoming more and more diverse. Especially by the expression of cysteine and thiol rich proteins such as metallothioneins in bacterial cells, a high rate of removal of metal ions from the environment is ensured. In this study, we evaluated the removal and reduction of hexavalent chromium Cr(VI)from aqueous solutions with the recombinant strains obtained by cloning the human metallothioneinsMT2A and MT3 into Escherichia coli Jm109.E. coli MT2A was the most effective strain in both Cr(VI) removal (89%in 25 mg/L Cr(VI) application) and Cr(VI) reduction (76%in 25 mg/L Cr(VI) application).Cr(VI) adsorbed per dry cell (at 25 mg/L) were 17 mg/g, 22 mg/g and 19 mg/g for Jm109, MT2A and MT3, respectively. Scanning electron microscope (SEM) images showed that the morphological structures of Cr(VI)treated cells were signi cantly damaged compared to control cells. Scanning transmission electron microscope (STEM) images showed black spots in the cytoplasm of cells treated with Cr(VI).From the shifts in the Fourier transform infrared spectroscopy analysis (FTIR) spectra of the cells treated with Cr(VI) compared to the control cells, it was determined that the groups interacting with Cr were amino, hydroxyl, methyl and sulfhydryl. When all these experimental data were evaluated together, it was concluded that all three species were effective in removing Cr(VI) from aqueous solutions and MT2A strain could be used as the most effective biotechnological tool.

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Section: Introductionmentioning

confidence: 99%

Bioreduction and bioremoval of hexavalent chromium by genetically engineered strains (Escherichia coli MT2A and Escherichia coli MT3)

Akkurt

Oğuz

Uçkun

2022

World J Microbiol Biotechnol

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“…Reductants of Cr(VI) like S(II) and Fe(II) can be produced by bacteria while oxalic acid can be produced by fungi. , The natural reduction of Cr(VI) has been shown by some fungal species through enzymatic reduction and sorption to mycelia. , The Cr-resistant fungus Paecilomyces lilacinus was shown to reduce 1.24 mg/L of Cr(VI) from tannery effluent to below the detection level in 18 h in both acidic and basic conditions . Additionally, the Cr-tolerant fungus Penicillium oxalicum SL2 was shown to reduce 40.6 and 96.1 mg/L of Cr(VI) in 48 and 96 h, respectively, from electroplating wastewater; a remarkable 89.6% of 217.1 mg/L Cr(VI) was also reduced from electroplating wastewater in 96 h . The reduction of Cr(VI) by Penicillium oxalicum SL2 was attributed to oxalic acid secreted by Penicillium oxalicum SL2 and by biomass uptake through amine, carboxyl, and phosphate functional groups .…”

Section: Involvement Of Cr(v)/cr(iv) Species In Chromium Redox Reacti...mentioning

confidence: 99%

“…75,87 The Cr-resistant fungus Paecilomyces lilacinus was shown to reduce 1.24 mg/L of Cr(VI) from tannery effluent to below the detection level in 18 h in both acidic and basic conditions. 88 Additionally, the Cr-tolerant fungus Penicillium oxalicum SL2 was shown to reduce 40.6 and 96.1 mg/L of Cr(VI) in 48 and 96 h, respectively, from electroplating wastewater; a remarkable 89.6% of 217.1 mg/L Cr(VI) was also reduced from electroplating wastewater in 96 h. 89 The reduction of Cr(VI) by Penicillium oxalicum SL2 was attributed to oxalic acid secreted by Penicillium oxalicum SL2 and by biomass uptake through amine, carboxyl, and phosphate functional groups. 87 Interestingly, Mn 2+ has been shown to promote Cr(VI) reduction by oxalic acid, posing another mechanism for the natural Cr(VI) reduction and application to wastewater remediation.…”

mentioning

confidence: 98%

Overlooked Role of Chromium(V) and Chromium(IV) in Chromium Redox Reactions of Environmental Importance

Bell

McDonald

et al. 2022

ACS EST Water

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Chromium (Cr) is a well-known heavy metal contaminant with toxicity highly dependent on its oxidation state. Hexavalent Cr (Cr(VI)) is a known carcinogen while trivalent chromium (Cr(III)) is significantly less toxic. The reduction of Cr(VI) and oxidation of Cr(III) in different compartments of the environment occur through intermediate species pentavalent Cr (Cr(V)) and tetravalent Cr (Cr(IV)), which are highly reactive. The environmental literature generally lacks information on Cr(V) and Cr(IV) species in various redox processes. This Perspective presents the aquatic chemistry of Cr(V) and Cr(IV), which includes their spectroscopic characterizations and kinetic behaviors under different environmental conditions. Examples are presented to demonstrate the possible existence of the intermediate Cr species in different systems of environmental importance such as the reduction of Cr(VI) by iron(II) (Fe(II)), molecules of natural organic matter (e.g., fulvic acids and carboxylic acids), and oxidation of Cr(III) by hydrogen peroxide, hypochlorite, and manganese oxides (MnO x ). The oxidation of organic pollutants by the Cr(VI)–S(IV) system is also discussed. This Perspective suggests in-depth investigations on the redox reactions of Cr relevant to environmental processes to shed light on the mechanisms of the generation of Cr(V) and Cr(IV) species and their roles in water decontamination.

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“…2020 ). It was also reported to be used to remediate toxic metals like chromium and lead ( Long et al . 2018 ; Tian et al .…”

Section: Introductionmentioning

confidence: 99%

A highly quality genome sequence of Penicillium oxalicum species isolated from the root of Ixora chinensis in Vietnam

Pham

et al. 2022

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Penicillium oxalicum has been reported as a multienzyme producing fungus and is widely used in industry due to great potential for cellulase release. Until now, there are only ten available genome assemblies of P. oxalicum species deposited in the Genbank database. In this study, the genome of the I1R1 strain isolated from the root of Ixora chinensis was completely sequenced by Pacbio Sequel sequencing technology, assembled into eight chromosomes with the genome size of 30.8 Mb, as well as a mitogenome of 26 Kb. The structural and functional analyses of the I1R1 genome revealed gene model annotations encoding an enzyme set involved in significant metabolic processes, along with cytochrome P450s and secondary metabolite biosynthesis. The comparative analysis of the P. oxalicum species based on orthology and gene family duplications indicated their large and closed pan genome of 9,500 orthologous groups. This is valuable data for future phylogenetic and population genomics studies.

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Characterization of Penicillium oxalicum SL2 isolated from indoor air and its application to the removal of hexavalent chromium

Cited by 33 publications

References 61 publications

Bioreduction and bioremoval of hexavalent chromium by genetically engineered strains (Escherichia coli MT2A and Escherichia coli MT3)

Bioreduction and bioremoval of hexavalent chromium by genetically engineered strains (Escherichia coli MT2A and Escherichia coli MT3)

Overlooked Role of Chromium(V) and Chromium(IV) in Chromium Redox Reactions of Environmental Importance

A highly quality genome sequence of Penicillium oxalicum species isolated from the root of Ixora chinensis in Vietnam

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