Characterization of perinatally born glutamatergic neurons of the mouse olfactory bulb based on NeuroD6 expression reveals their resistance to sensory deprivation

Angelova, Alexandra; Platel, Jean‐Claude; Béclin, Christophe; Cremer, Harold; Coré, Nathalie

doi:10.1002/cne.24621

Cited by 7 publications

(10 citation statements)

References 51 publications

(98 reference statements)

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“…1HI). As observed before, we found in control condition a slight expansion of the size of the glomerulus and of the distance between neurons [1] and a shrinkage under UNO [12].…”

Section: Resultssupporting

confidence: 87%

“…The advent of in vivo two-photon imaging combined with genetic labeling of specific populations allows to unequivocally address this issue. Using this approach, we previously demonstrated that glomerular neuronal populations are differentially affected by UNO since periglomerular glutamatergic interneurons do not undergo cell death under sensory deprivation, while GABAergic neurons do [12]. Here, we followed TH and other GABAergic neurons before, during and after reversible sensory deprivation.…”

Section: Introductionmentioning

confidence: 99%

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Sensory deprivation triggers phenotypic adjustment of dopaminergic interneurons in the mouse olfactory bulb

Angelova

Tiveron

Loizeau

et al. 2022

Preprint

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Olfactory sensory activity is a main factor factor controlling intergration and survival of neurons in the olfactory bulb. However, its impact on specific neuronal subtypes is unclear. Using reversible unilateral naris closure in concert with longitudinal in vivo imaging we show here that newborn GABAergic interneurons undergo significant cell death under sensory deprivation. In contrast, dopaminergic OB neurons survive under deprivation, but react with a reversible downregulation of tyrosine hydroxylase expression.long abstractNeurogenesis persists in the mammalian subventricular zone after birth, producing various populations of olfactory bulb (OB) interneurons. These include GABAergic and mixed dopaminergic/GABAergic double neurotransmitter neurons for the glomerular layer. While olfactory sensory activity is one of the main factors controlling newborn neuron integration, its effect on specific neuronal subtypes is far less clear. Here we use a reversible unilateral deprivation paradigm in combination with longitudinal in vivo imaging to characterize the behavior of newborn glomerular neurons. We find that a substantial fraction of purely GABAergic neurons die after four weeks of sensory deprivation. Tyrosine Hydroxylase (TH) positive dopaminergic/ GABAergic neurons show no signifficant cell death under deprivation, but react with an important decrease in TH expression levels. Importantly, this effect reverses after naris reopening, pointing to a specific adaptation of this neuron population to the level of sensory activity. We conclude that sensory deprivation induces adjustments in the excitation/inhibition balance of the OB implicating cell death and adaptation of neurotransmitter use in specific neuron types.

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“…1HI). As observed before, we found in control condition a slight expansion of the size of the glomerulus and of the distance between neurons [1] and a shrinkage under UNO [12].…”

Section: Resultssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Sensory deprivation triggers phenotypic adjustment of dopaminergic interneurons in the mouse olfactory bulb

Angelova

Tiveron

Loizeau

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…neurons connected to the same glomerulus, whose mechanism and role remain elusive ( 11, 12, 13 ).The glomerular layer is populated by various interneuron subtypes. They are mainly GABAergic, but a small group of glutamatergic interneurons, labeled through the ND6 promoter, project locally in the OB ( 14 ) and could provide a unique opportunity to better understand this glomerular network.…”

Section: Main Textmentioning

confidence: 99%

“…The ND6 promoter drives Cre expression in all glutamatergic neurons in the OB, including the population of vesicular glutamate transporter positive glomerular neurons (Fig. 1B) ( 14 ). We used two-photon calcium imaging in anesthetized animals and recorded consecutively odor-evoked responses in three to six imaging planes in the glomerular layer to sample a larger population of ND6+ neurons (Fig.…”

Section: Main Textmentioning

confidence: 99%

Dynamic intraglomerular neuronal ensembles represent odor identity and concentration

Loizeau,

Angelova,

Cremer

et al. 2023

Preprint

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The olfactory system has been extensively studied from an anatomical and functional point of view, yet how and where certain basic odor characteristics such as identity and concentration are represented in the brain remains poorly understood. The glomerular layer of the olfactory bulb is the first brain region that integrates olfactory signals and enables a topographic representation of odor identity. We investigated this odor encoding at the intraglomerular network level using genetic labeling, in vivo imaging, and computational methods. Our analyses demonstrated that glomerular glutamatergic neurons encode both odor identity and concentration. Furthermore, in vivo structural and functional imaging of sister neurons revealed the emergence of intraglomerular neuronal ensembles governed by odor identity and concentration. These findings revealed a novel network mechanism that enables the simultaneous coding of odor identity and concentration within glomerular modules, suggesting a potential mechanism for odor decorrelation and a new model for odor information processing in the olfactory bulb.One-sentence summaryUnraveling the scent puzzle: Discovery of intraglomerular neuronal ensembles sheds light on odor representation in the brain.

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“…S8D ). These distal interacting cEnhancers are marked with open chromatin in excitatory neurons, cells known for high Neurod6 expression 57 . TF motifs enriched at cEnhancers with TF expression patterns that match cEnhancer activity implicated many known brain developmental regulators as activators for these enhancers ( Fig.…”

Section: Introductionmentioning

confidence: 99%

Integrative analysis of the 3D genome and epigenome in mouse embryonic tissues

Zemke

Chen

et al. 2022

Preprint

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While a rich set of putative cis-regulatory sequences involved in mouse fetal development has been annotated recently based on chromatin accessibility and histone modification patterns, delineating their role in developmentally regulated gene expression continues to be challenging. To fill this gap, we mapped chromatin contacts between gene promoters and distal sequences genome-wide in seven mouse fetal tissues, and for one of them, across six developmental stages. We identified 248,620 long-range chromatin interactions centered at 14,138 protein-coding genes and characterized their tissue-to-tissue variations as well as developmental dynamics. Integrative analysis of the interactome with previous epigenome and transcriptome datasets from the same tissues revealed a strong correlation between the chromatin contacts and chromatin state at distal enhancers, as well as gene expression patterns at predicted target genes. We predicted target genes of 15,098 candidate enhancers, and used them to annotate target genes of homologous candidate enhancers in the human genome that harbor risk variants of human diseases. We present evidence that schizophrenia and other adult disease risk variants are frequently found in fetal enhancers, providing support for the hypothesis of fetal origins of adult diseases.

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Characterization of perinatally born glutamatergic neurons of the mouse olfactory bulb based on NeuroD6 expression reveals their resistance to sensory deprivation

Cited by 7 publications

References 51 publications

Sensory deprivation triggers phenotypic adjustment of dopaminergic interneurons in the mouse olfactory bulb

Sensory deprivation triggers phenotypic adjustment of dopaminergic interneurons in the mouse olfactory bulb

Dynamic intraglomerular neuronal ensembles represent odor identity and concentration

Integrative analysis of the 3D genome and epigenome in mouse embryonic tissues

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