Characterization of phosphate-solubilizing bacteria isolated from calcareous soils

Liu, Zhiguang; Li, Yuncong; Zhang, Shouan; Fu, Yuqing; Fan, Xiangtao; Patel, J. S.

doi:10.1016/j.apsoil.2015.08.003

Cited by 121 publications

(83 citation statements)

References 38 publications

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“…This is in agreement with Liua et al. , who demonstrated three groups of bacterial strains that had different kinetics of phosphorus solubilization and one of these groups consists of three different strains of B. megaterium that solubilized Ca 3 (PO 4 ) 2 in the same manner like strain reported herewith.…”

Section: Resultssupporting

confidence: 93%

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In situ solubilization of phosphorus‐bearing raw materials by Bacillus megaterium

Wyciszkiewicz

Saeid

Chojnacka

2017

Engineering in Life Sciences

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This paper presents the results of in situ studies on solubilization of different phosphorus-bearing raw materials by application of natural ability to produce organic acids by Bacillus megaterium. Poultry bones as well as fish bones were used as renewable sources of phosphates. Morocco phosphorite was used as nonrenewable sources of phosphates. Glass columns, filled with the soil mixed with different doses (1, 5, and 10%) of mentioned sources of phosphorus, were used as a medium for solubilization. It was found that the amount of released phosphorus (determined in the eluent and expressed as P 2 O 5 ) significantly increased in the cases of columns where B. megaterium was used, when compared with the control group (without microflora). Higher doses of phosphorus-bearing material used in the experiment influenced in the release of higher amount of phosphorus. The highest effectiveness of solubilization was found in the case of poultry bones. The experiment was repeated for poultry bones but with the supplementation of nutrients. It was found that the delivery of nutrients had a strong effect on the increase of effectiveness of solubilization. Two times higher amount of phosphorus (express as P 2 O 5 ) was released from the hydroxyapatite structure of bones. It was confirmed that poultry bones could serve as a source of phosphates in microbial solubilization performed in in situ.

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Section: Resultssupporting

confidence: 93%

“…Similar findings were reported by Liua et al. , the additional nutrition statistically significant improved the solubilization of Ca 3 (PO 4 ) 2 .…”

Section: Resultssupporting

confidence: 90%

In situ solubilization of phosphorus‐bearing raw materials by Bacillus megaterium

Wyciszkiewicz

Saeid

Chojnacka

2017

Engineering in Life Sciences

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“…Phosphorus (P) is the second limiting nutrient required for plant growth and development involved in important metabolic pathways like nutrient uptake, biological oxidation, and energy metabolism (Nesme et al, 2018). The total P in soil accounts roughly for 0.04-0.1% (w/w), only a very tiny proportion of P (soluble H 2 PO 4 − or HPO 4 2− ) can directly be assimilated by plants (Chen et al, 2008), as the large portion of P in soils exists in inorganic insoluble form [e.g., Ca 3 (PO 4 ) 2 ] and organic insoluble/soluble form (e.g., phytate and nucleic acid) (Liu et al, 2015;Neal et al, 2017). The input of P to soil is mainly via fertilization, and both abiotic substance (mainly inorganic P mineral) and organic P compounds (e.g., animal, plant, and microbes residues and wastes) are widely used in agricultural ecosystems (Lim et al, 2007;Jorquera et al, 2013;Fraser et al, 2015), and these extraneously added P including inorganic P (IP) and organic P (OP) get converted into salts and become insoluble by bounding to Ca, Al, Mg, Mn, and Fe (Liu et al, 2015;Reddy et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Phosphorus Solubilizing Bacteria With Multiple Phosphorus Sources Utilizing Capability and Their Potential for Lead Immobilization in Soil

et al. 2020

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Phosphorus solubilizing bacteria (PSB) can promote the level of plant-absorbable phosphorus (P) in agro-ecosystems. However, little attention has been paid to PSB harboring abilities in utilizing multiple phosphorus sources and their potentials for heavy metal immobilization. In this study, we applied the strategy of stepwise acclimation by using Ca 3 (PO 4) 2 , phytate, FePO 4 , and AlPO 4 as sole P source. We gained 18 PSB possessing abilities of multiple P sources utilization, and these bacteria belonged to eight genera (Acinetobacter, Pseudomonas, Massilia, Bacillus, Arthrobacter, Stenotrophomonas, Ochrobactrum, and Cupriavidus), and clustered to two apparent parts: Gram-positive bacteria and Gram-negative bacteria. The isolate of Acinetobacter pittii gp-1 presented good performance for utilizing Ca 3 (PO 4) 2 , FePO 4 , AlPO 4 , and phytate, with corresponding P solubilizing levels were 250.77, 46.10, 81.99, and 7.91 mg/L PO 4 3−-P, respectively. The PSB A. pittii gp-1 exhibited good performance for solubilizing tricalcium phosphate in soil incubation experiments, with the highest values of water soluble P and available P were 0.80 and 1.64 mg/L, respectively. Additionally, the addition of A. pittii gp-1 could promote the immobilization of lead (Pb), and the highest Pb immobilization efficiency reached 23%. Simultaneously, we found the increases in abundances of both alkaline phosphatase gene (phoD) and β-propeller phytase gene (bpp) in strain gp-1 added soils. Besides, we observed the expression up-regulation of both pyrroloquinoline quinone gene (pqq) and polyphosphate kinases gene (ppk), with the highest relative expression levels of 18.18 and 5.23, respectively. We also found the polyphosphate particles using granule staining. To our knowledge, our findings first suggest that the solubilizing of tricalcium phosphate by phosphorus solubilizing bacterium belonging to Acinetobacter is coupled with the synthesis of polyphosphate. Taken together, A. pittii gp-1 could be a good candidate in improving soil fertility and quality.

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“…National Botanical Research Institute's Phosphate (NBRIP) growth medium was prepared by weighing 10 g of glucose, 5 g of tricalcium phosphate, 5 g of magnesium chloride hexahydrate, 0.25 g of magnesium sulphate heptahydrate, 0.2 g of potassium chloride, 0.1 g of ammonium sulphate and 15 g of bacteriological agar. The isolates were characterized as P-solubilizing bacteria based on the clear zone formation (Liu et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

Selecting the most effective plant growth-promoting bacteria from oil palm (Elaeis guineensis Jacq) roots

Zakry

Ammal

Malahubban

et al. 2019

J Bangladesh Agric Univ

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A total of 30 bacterial isolates were isolated from soil, rhizoplane, and internal tissue of oil palm roots. The isolates were qualitatively tested for their potential to fix N2, solubilize inorganic P and K, and produce phytohormone indole-3-acetic acid. Of the 30 isolates, six isolates were able to exhibit multiple beneficial traits. All six isolates were then identified based on fatty acid methyl esters profile as Escherichia coli strain EX2, Serratia sp. strain EN1, Pantoea ananatis strain EN3, Bacillus sp. strain EN5, Pantoea ananatis strain EN8 and Pantoea sp. strain EN9. Subsequently, all shortlisted isolates were evaluated for plant growth-promoting potential by using shallot as a test plant. The plant test showed no significant difference (p>0.05) between inoculated and uninoculated plants except for Pantoea sp. strain EN9 inoculation which increased significantly (p<0.05) total root length over uninoculated control. Host specificity and IAA capacity of the isolates may be among the important factors affecting their effectiveness in plant growth promotion. J Bangladesh Agril Univ 17(3): 344–348, 2019

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Characterization of phosphate-solubilizing bacteria isolated from calcareous soils

Cited by 121 publications

References 38 publications

In situ solubilization of phosphorus‐bearing raw materials by Bacillus megaterium

In situ solubilization of phosphorus‐bearing raw materials by Bacillus megaterium

Isolation and Characterization of Phosphorus Solubilizing Bacteria With Multiple Phosphorus Sources Utilizing Capability and Their Potential for Lead Immobilization in Soil

Selecting the most effective plant growth-promoting bacteria from oil palm (Elaeis guineensis Jacq) roots

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